Plant Culture
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| Author | : Robert H. Smith |
| Publisher | : Academic Press |
| Total Pages | : 190 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323160476 |
Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
| Author | : Robert N. Trigiano |
| Publisher | : CRC Press |
| Total Pages | : 608 |
| Release | : 2016-03-30 |
| Genre | : Medical |
| ISBN | : 1439896143 |
Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol
| Author | : John H. Dodds |
| Publisher | : International Potato Center |
| Total Pages | : 260 |
| Release | : 1985-10-31 |
| Genre | : Science |
| ISBN | : 9780521315166 |
The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.
| Author | : Jared Rosenbaum |
| Publisher | : New Society Publishers |
| Total Pages | : 326 |
| Release | : 2022-11-22 |
| Genre | : Nature |
| ISBN | : 1550927736 |
Reconnect. Restore. Reciprocate. Repairing landscapes and reconnecting us to the wild plant communities around us. Integrating restoration practices, foraging, herbalism, rewilding, and permaculture, Wild Plant Culture is a comprehensive guide to the ecological restoration of native edible and medicinal plant communities in Eastern North America. Blending science, practice, and traditional knowledge, it makes bold connections that are actionable, innovative, and ecologically imperative for repairing both degraded landscapes and our broken cultural relationship with nature. Coverage includes: Understanding and engaging in mutually beneficial human-plant connections Techniques for observing the land's existing and potential plant communities Baseline monitoring, site preparation, seeding, planting, and maintaining restored areas Botanical fieldwork restoration stories and examples Detailed profiles of 209 native plants and their uses. Both a practical guide and an evocative read that will transport you deep into the natural landscape, Wild Plant Culture is an essential toolkit for gardeners, farmers, and ecological restoration practitioners, highlighting the important role humans play in tending and mending native plant communities.
| Author | : Michael R. Davey |
| Publisher | : John Wiley & Sons |
| Total Pages | : 358 |
| Release | : 2010-05-20 |
| Genre | : Science |
| ISBN | : 9780470686515 |
The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.
| Author | : J. Benton Jones Jr. |
| Publisher | : CRC Press |
| Total Pages | : 420 |
| Release | : 2007-08-03 |
| Genre | : Science |
| ISBN | : 1420007394 |
While tomatoes continue to be one of the most widely grown plants, the production and distribution of tomato fruits have been changing worldwide. Smaller, flavorful tomatoes are becoming more popular than beefsteak tomatoes, greenhouse-grown tomatoes have entered the marketplace, and home gardeners are using the Internet to obtain information for g
| Author | : Duong Tan Nhut |
| Publisher | : Springer Nature |
| Total Pages | : 400 |
| Release | : 2022-04-05 |
| Genre | : Science |
| ISBN | : 9811664986 |
This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.
| Author | : M. K. Razdan |
| Publisher | : Science Publishers |
| Total Pages | : 394 |
| Release | : 2003 |
| Genre | : Science |
| ISBN | : 9781578082377 |
Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.
| Author | : R. A. Dixon |
| Publisher | : IRL Press |
| Total Pages | : 254 |
| Release | : 1994 |
| Genre | : Cell culture |
| ISBN | : 9780199634026 |
Plant cell culture techniques are used increasingly in basic research for plant exploitation in industry, including for example, genetic engineering and micropropagation. The rapidly developing role of plant cell culture has necessitated this new edition of a widely acclaimed book. It covers a wide range of methods central to the exploitation of plant cell cultures in fundamental and applied research. This thoroughly revised work retains the combination of giving and explaining the general principles involved with the concise description of specific protocols, with appeal to a broad readership, that made the first edition so successful. Internationally recognized experts describe the techniques used for isolating and manipulating cell cultures, and the central importance in plant biotechnology. The book will be of major interest to researchers in plant sciences in general, and specifically to botany, plant physiology, and biotechnology students.
| Author | : Robert N. Trigiano |
| Publisher | : CRC Press |
| Total Pages | : 476 |
| Release | : 1999-11-10 |
| Genre | : Science |
| ISBN | : 9780849320293 |
Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include "Micropropagation of Dieffenbachia," "Micropropagation and in vitro flowering of rose," "Propagation from nonmeristematic tissue-organogenesis," "Variation in culture" and "Tissue culture of ferns." It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.
