Physical Principles And Techniques Of Protein Chemistry Part C
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| Author | : Sydney Leach |
| Publisher | : Elsevier |
| Total Pages | : 639 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323141145 |
Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry.
| Author | : Sydney Leach |
| Publisher | : Elsevier |
| Total Pages | : 545 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323141129 |
Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins' dynamic properties and rate constants. Other chapters deal with the evaluation of proteins' dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.
| Author | : Sydney Leach |
| Publisher | : Elsevier |
| Total Pages | : 508 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323141137 |
Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.
| Author | : J.Ellis Bell |
| Publisher | : CRC Press |
| Total Pages | : 377 |
| Release | : 2018-05-04 |
| Genre | : Medical |
| ISBN | : 135109372X |
This book has been written in part with the aim of providing a text which will be useful in teaching the biochemical applications of spectroscopy. This book will be of particular use to the biochemist or biologist who does not have a background in spectroscopy, but desires to find out what sort of information spectroscopy can provide. Attention was limited to those techniques most frequently used, and which at present have the widest applications.
| Author | : Graham Barrett |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 694 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 9400948328 |
Amino acids are featured in course syllabuses and in project and research work over a wide spectrum of subject areas in chemistry and biology. Chemists and biochemists using amino acids have many common needs when they turn to the literature for comprehensive information. Among these common interests, analytical studies, in particular, have undergone rapid development in recent years. All other chemical and biochemical aspects of amino acids - synthesis, properties and reactions, preparation of derivatives for use in peptide synthesis, racemization and other fundamental mechanistic knowledge - have been the subject of vigorous progress. This book offers a thorough treatment of all these developing areas, and is structured in the belief that biochemists, physiologists and others will profit from access to information on topics such as the physical chemistry of amino acid solutions, as well as from thorough coverage of amino acid metabolism, biosynthesis and enzyme inhibition; and that chemists will find relevant material in biological areas as well as in the analysis, synthesis and reactions of amino acids.
| Author | : W. Hoppe |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 967 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 3642688772 |
What is biophysics? As with all subjects which straddle traditional boundaries between fields, it eludes a precise definition. Furthermore, it is impossible to do biophysics without having a certain foundation of knowledge in biology, physics, physical chemistry, chemistry and biochemistry. One approach to a biophysics textbook would be to refer the student to the literature of these neighboring fields, and to leave the selection of the appropriate supplementary material up to the student. The editors of this volume are of the opinion that it is more useful and less time-consuming to present a selection of the supplementary knowledge, in concentrated form, together with the subject matter specific to biophysics. The reader will thus find in this book introductions to such subjects as the structure and function of the cell, the chemical structure of biogenic macromolecules, and even theoretical chemistry. What, indeed, is biophysics? Must we consider it to include physiology, electromedicine, radiation medicine, etc. ? The field has evolved continuously in recent years. Molecular understanding oflife processes has come more and more to the fore. Just as the field of molecular physics has developed to describe structures and processes in the realm of non-living systems, there has been a corresponding development of molecular biophysics.
| Author | : David Freifelder |
| Publisher | : Macmillan |
| Total Pages | : 786 |
| Release | : 1982-08-15 |
| Genre | : Medical |
| ISBN | : 9780716714446 |
Suitable for advanced undergraduate and graduate students in biochemistry, this book provides clear, concise, well-exampled descriptions of the physical methods that biochemists and molecular biologists use.
| Author | : Herbert Weissbach |
| Publisher | : Elsevier |
| Total Pages | : 739 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323141706 |
Molecular Mechanisms of Protein Biosynthesis is a collection of papers dealing with cell-free systems at the molecular level, including transfer RNA; the initiation, elongation, and termination processes; ribosome structure and function; mRNA translation; and DNA-directed in vitro protein synthesis. A couple of papers review tRNA, aminoacyl-tRNA synthetases, and aspects of ribosome structure. One paper discusses affinity labeling in the study of binding and catalytic sites of large complex and heterogeneous systems such as the ribosome. The investigator should be aware of the chemically reactive or photoactivatible analogue reacting specifically with one or more ribosomal components. This reaction should be determined if it is dependent on the correct binding of the affinity label at the functional site. Another paper describes the series of reactions in protein synthesis as the process by which the ribosome moves relative to the messenger RNA. Other papers discuss messenger RNA and its translation, DNA-dependent cell-free protein synthesis, as well as the genetics of the translational apparatus. The collection will benefit microbiologists, biotechnologists, and academicians connected with the biological sciences.
| Author | : R Fraser |
| Publisher | : Elsevier |
| Total Pages | : 649 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323143997 |
Conformation in Fibrous Proteins: And Related Synthetic Polypeptides provides a comprehensive and critical account of conformation in fibrous proteins and synthetic polypeptides in the solid state. Physical methods of determining conformation are discussed, and relevant results from studies of synthetic polypeptides and fibrous proteins are presented. Comprised of 18 chapters divided into three sections, this book opens with a discussion on the theory and technique of X-ray diffraction applicable to the study of conformation in fibrous materials, along with electron diffraction, electron microscopy, optical diffraction, and infrared spectrophotometry. The procedures used for conformation analysis and prediction are also outlined. The following chapters consider optimization techniques and other methods for elucidating conformation in fibrous proteins and synthetic polypeptides; the use of synthetic polypeptides as models of fibrous proteins; and conformation in fibrous proteins such as silks, collagens, myofibrillar proteins, and keratins. This monograph will be a valuable source of information for molecular biologists.
| Author | : H. Malissa |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 338 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 3709186307 |
th This proceedings volume of the 8 International Microchemical Symposium contains the plenary and keynote lectures delivered at the conference. Besides basic and historic aspects the following major topics are covered: "Microchemistry Arts and Archeology" in "Microchemistry in Life Sciences" "Microchemistry Sciences" in Environmental "Microchemistry in Material Sciences" "Instrumentation, Methods and Automation in Microchemistry". The papers show the present state of microchemistry and the development of this field since the pioneer days of Fritz Pregl and Friedrich Emich. Today microchemistry is a different science as compared to the Pregl and Emich days, for it combines many disciplines like chemistry, physics, mathematics, informatics, biology and does not only mean microanalysi- even if it is still predominant and the best tool for elucidation of the microcosmos. Due to this development modern microchemistry plays an important role in science and technology. It had been the intention of the Scientific th Executive Committee to demonstrate this at the 8 International Micro chemical Symposium with the goal to encourage interdisciplinary communication and stimulate discussion.
