Optical Microscopy For Biology
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| Author | : Alan J. Lacey |
| Publisher | : OUP Oxford |
| Total Pages | : 475 |
| Release | : 1999-06-03 |
| Genre | : Science |
| ISBN | : 0191565725 |
Since the first edition of Light Microscopy in Biology: A Practical approach was published, techniques in modern light microscopy have improved considerably. This fully updated edition includes revised topics from the first edition as well as coverage of techniques and technologies that have been developed since it was published. As before, the book starts with an explanation of the basic techniques, and goes on to describe current methods in: chromosome microscopy, immunohistochemistry, fluorescence microscopy, image building and video microscopy. Totally new topics covered include: confocal microscopy, calcium and pH imaging, microinjection techniques and nanovid microscopy. There are also whole chapters now devoted to reflection contrast microscopy and histomorphometry. This new edition will be of great interest to postgraduate and postdoctoral researchers in biomedicine and cell biology - both those experienced with light microscopic techniques and newcomers to the field.
| Author | : Chris Hallski |
| Publisher | : Master Books |
| Total Pages | : 93 |
| Release | : 2019-10-29 |
| Genre | : Religion |
| ISBN | : 9781683441915 |
Providing an overview of God's world through a microscope, this book gives a brief history of microscopes before diving into seeing the world through one. Starting with their simple origins in the 13th century as magnifying glasses and exploring some of the many modern varieties of imaging, we explore how they are used and some of what may be seen through one now.Filled with full-color microscopic images of varied animals, insects, plants and fungi, and microorganisms, as well as detailed information for using the modern microscope in the classroom.Discusses examples of stained and unstained slide samples, brightfield, darkfield, and phase contrast microscopy.Includes practical tips about the use of the microscope and labels many of the slide images for easier identification of microscopic structures.Though this is an independent text that can be used with any biology study, it also serves as a companion book in the Master's Class Biology: The Study of Life From a Christian Worldview high school course available from Master Books®. Those who purchase this book would not have to purchase a microscope in order to fulfill the requirements.
| Author | : Greenfield Sluder |
| Publisher | : Elsevier |
| Total Pages | : 626 |
| Release | : 2007-04-26 |
| Genre | : Science |
| ISBN | : 0080544347 |
The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe's theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. - Expands coverage to include discussion of confocal microscopy not found in the previous edition - Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods
| Author | : Alberto Diaspro |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 252 |
| Release | : 2010-11-25 |
| Genre | : Science |
| ISBN | : 3642151752 |
In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.
| Author | : James Pawley |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 639 |
| Release | : 2013-04-17 |
| Genre | : Science |
| ISBN | : 1475753489 |
This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.
| Author | : Randy O. Wayne |
| Publisher | : Academic Press |
| Total Pages | : 373 |
| Release | : 2013-12-16 |
| Genre | : Science |
| ISBN | : 0124115365 |
The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. - Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope - Clearly develops all ideas from historical and logical foundations - Laboratory exercises included to assist the reader with practical applications - Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope
| Author | : Brian Herman |
| Publisher | : Wiley-Liss |
| Total Pages | : 708 |
| Release | : 1990-05-10 |
| Genre | : Science |
| ISBN | : |
Optical Microscopy for Biology presents an up-to-date, comprehensive description of new methods in optical microscopy for observing cellular structure and function at the level of single intact cells or tissue. Contributors cover confocal microscopy and optional sectioning of cells, fluorophores and characterization of various fluorescent probes and detector characterization. They also discuss a number of applications to current biological problems. In addition, Optical Microscopy for Biology includes a preview of the latest advances and newest developments in the technology of optimal microscopy, including four-dimensional miscroscopy, multiparameter and multimode digitized video microscopy, digitized fluorescence polarization, and near field microscopy.
| Author | : Barry R. Masters |
| Publisher | : Oxford University Press |
| Total Pages | : 895 |
| Release | : 2008-05-19 |
| Genre | : Science |
| ISBN | : 0198036825 |
The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.
| Author | : Gordon S. Kino |
| Publisher | : Academic Press |
| Total Pages | : 353 |
| Release | : 1996-09-18 |
| Genre | : Science |
| ISBN | : 008052978X |
This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. The book concentrates mainly on two instruments: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A comprehensive discussion of the theory and design of the Near-Field Scanning Optical Microscope (NSOM) is also given. The text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes. - Provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers - Explains many practical applications of scanning optical and interference microscopy in such diverse fields as biology and semiconductor metrology - Discusses in theoretical terms the origin of the improved depth and transverse resolution of scanning optical and interference microscopes with emphasis on the practical results of the theoretical calculations - Considers the practical aspects of building a confocal scanning or interference microscope and explores some of the design tradeoffs made for microscopes used in various applications - Discusses the theory and design of near-field optical microscopes - Explains phase imaging in the scanning optical and interference microscopes
| Author | : Douglas B. Murphy |
| Publisher | : |
| Total Pages | : 538 |
| Release | : 2013 |
| Genre | : |
| ISBN | : |
