Neuromuscular Junctions in Drosophila

Neuromuscular Junctions in Drosophila
Author:
Publisher: Academic Press
Total Pages: 317
Release: 1999-04-29
Genre: Science
ISBN: 0080857779

Neuromuscular Junctions in Drosophila gathers the main contributions that research using the fruit fly Drosophila melanogaster has made in the area of synapse development, synapse physiology, and excitability of muscles and nerve cells. The chapters in this book represent a synthesis of major advances in our understanding of neuronal development and synaptic physiology, which have been obtained using the above approach.This book is directed to the general neuroscience audience: researchers, instructors, graduate students, and advanced undergraduates who are interested in the mechanisms of synapse development and physiology. However, the book will also be a valuable resource for those that use the fruit fly as a model system in their laboratories.Key Features* Synthesizes the genetic approaches used to study synaptic development and function at the neuromuscular junction, using flies as a model system* Covers major recent advances in muscle development, pathfinding, synapse maturation and plasticity, exo- and endocytosis, and ion channel function* Written in clear language that is easily understandable to readers not already familiar with fruit fly research* Includes numerous diagrams and extensive reference lists

The Fly Neuromuscular Junction: Structure and Function

The Fly Neuromuscular Junction: Structure and Function
Author: Vivian Budnik
Publisher: Academic Press
Total Pages: 424
Release: 2006-10-31
Genre: Medical
ISBN: 9780123736130

The Drosophila larval neuromuscular junction (NMJ) has become one of the most powerful model systems to ask key neurobiological questions. This synapse is unparalleled by its accessibility, its simplicity, and the ability to manipulate genes important for synapse development and function. Its synapses have properties shared by many organisms including humans. The vast majority of genes that when mutated cause congenital disorders of the nervous system in humans, are present in the fruit fly genome, and fly models of human disorders are available. Thus, this preparation is a powerful tool to understand the normal function of these genes. This book reviews outstanding work by recognized leaders in the fields of Drosophila cellular neurogenetics including developmental neurobiology, mechanisms of synaptic function, and experience dependent changes at synapses. The book also includes step-by-step protocols to study the cellular biology of the NMJ, making it a vital resource for researchers beginning their investigations with this system, for those who are training students and postdoctoral fellows in this area, or simply as a general reference material for neuroscientists and neuroscience professors in general. * Provides a synthesis of the main topics in modern neurogenetics * Includes step-by-step protocols for the use of the Drosophila NMJ system in neurobiology lab research * Offers genetic approaches to study synapse development and function *

Muscle Development in Drosophilia

Muscle Development in Drosophilia
Author: Helen Sink
Publisher: Springer Science & Business Media
Total Pages: 224
Release: 2006-02-02
Genre: Science
ISBN: 9780387300535

The different aspects of muscle development are considered from cellular, molecular and genetic viewpoints, and the text is supported by black/white and color illustrations. The book will appeal to those studying muscle development and muscle biology in any organism.

Exocytosis and Endocytosis

Exocytosis and Endocytosis
Author: Andrei I. Ivanov
Publisher: Springer Science & Business Media
Total Pages: 407
Release: 2008
Genre: Medical
ISBN: 1588298655

In this book, skilled experts provide the most up-to-date, step-by-step laboratory protocols for examining molecular machinery and biological functions of exocytosis and endocytosis in vitro and in vivo. The book is insightful to both newcomers and seasoned professionals. It offers a unique and highly practical guide to versatile laboratory tools developed to study various aspects of intracellular vesicle trafficking in simple model systems and living organisms.

Drosophila Protocols

Drosophila Protocols
Author: William Sullivan
Publisher: CSHL Press
Total Pages: 798
Release: 2000
Genre: Medical
ISBN: 9780879695866

This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

Behavioral Genetics of the Fly (Drosophila Melanogaster)

Behavioral Genetics of the Fly (Drosophila Melanogaster)
Author: Josh Dubnau
Publisher: Cambridge University Press
Total Pages: 309
Release: 2014-06-26
Genre: Medical
ISBN: 1107009030

A comprehensive portrayal of the behaviour genetics of the fruit fly (Drosophila melanogaster) and the methods used in these studies.

RNA Detection

RNA Detection
Author: Imre Gaspar
Publisher: Humana Press
Total Pages: 492
Release: 2017-11-15
Genre: Medical
ISBN: 9781493972128

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

Anesthetic Pharmacology

Anesthetic Pharmacology
Author: Alex S. Evers
Publisher: Cambridge University Press
Total Pages: 2902
Release: 2011-03-10
Genre: Medical
ISBN: 1139497022

In recent years our understanding of molecular mechanisms of drug action and interindividual variability in drug response has grown enormously. Meanwhile, the practice of anesthesiology has expanded to the preoperative environment and numerous locations outside the OR. Anesthetic Pharmacology: Basic Principles and Clinical Practice, 2nd edition, is an outstanding therapeutic resource in anesthesia and critical care: Section 1 introduces the principles of drug action, Section 2 presents the molecular, cellular and integrated physiology of the target organ/functional system and Section 3 reviews the pharmacology and toxicology of anesthetic drugs. The new Section 4, Therapeutics of Clinical Practice, provides integrated and comparative pharmacology and the practical application of drugs in daily clinical practice. Edited by three highly acclaimed academic anesthetic pharmacologists, with contributions from an international team of experts, and illustrated in full colour, this is a sophisticated, user-friendly resource for all practitioners providing care in the perioperative period.

The Embryonic Development of Drosophila melanogaster

The Embryonic Development of Drosophila melanogaster
Author: Jose A. Campos-Ortega
Publisher: Springer Science & Business Media
Total Pages: 237
Release: 2013-03-09
Genre: Science
ISBN: 3662024543

" . . . but our knowledge is so weak that no philosoph er will ever be able to completely explore the nature of even a fly . . . " * Thornas Aquinas "In Syrnbolurn Apostolorum" 079 RSV p/96 This is a monograph on embryogenesis of the fruit fly Drosophi la melanogaster conceived as a reference book on morphology of embryonie development. A monograph of this extent and con tent is not yet available in the literature of Drosophila embryolo gy, and we believe that there is areal need for it. Thanks to the progress achieved during the last ten years in the fields of devel opmental and molecular genetics, work on Drosophila develop ment has considerably expanded creating an even greater need for the information that we present here. Our own interest for wildtype embryonie development arose several years ago, when we began to study the development of mutants. While those studies were going on we repeatedly had occasion to state in sufficiencies in the existing literature about the embryology of the wildtype, so that we undertook investigating many of these problems by ourselves. Convinced that several of our colleagues will have encountered similar difficulties we decided to publish the present monograph. Although not expressely recorded, Thomas Aquinas probably referred to the domestic fly and not to the fruit fly. Irrespective of which fly he meant, however, we know that Thomas was right in any case.

Disorders of Voluntary Muscle

Disorders of Voluntary Muscle
Author: George Karpati
Publisher: Cambridge University Press
Total Pages: 800
Release: 2001-07-12
Genre: Medical
ISBN: 9780521650625

Rewritten and redesigned, this remains the one essential text on the diseases of skeletal muscle.