A real time ship simulation investigation of the proposed design for deepening and widening the Alafia River Channel and turning basin, Tampa Bay, Florida, was conducted. Existing and proposed channel depths are 30 ft and 41 ft, respectively. The purpose of the study was to determine effects of proposed improvements on navigation with larger design ships and to assist in optimizing channel width and turning basin configuration required to efficiently navigate the study area. A numerical model of the existing channel from Hillsborough Bay Channel Cut C to the harbor area was developed. The model was verified by two members of the Tampa Bay Pilots Association. Numerical models of two plans were also developed. The investigation was conducted in Vicksburg, MS, on the U.S. Army Engineer Waterways Experiment Station ship simulator.