Molecular Methods In Plant Biology A Comprehensive Book On Biotechnicological
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Author | : Krishnananda Pralhad Ingle |
Publisher | : I K International Pvt Ltd |
Total Pages | : 104 |
Release | : 2020-12-30 |
Genre | : Science |
ISBN | : 938676881X |
This book integrates the experimental procedures and theoretical principles for undergraduate, postgraduate, academicians and researchers in the area of agriculture and life sciences. The experiments have been updated and extended to reflect developments in the respective fields. In-text worked examples are again used to enhance student’s understanding of each topic. The book is designed to provide students with the experience of how scientists use their knowledge to understand real-world science related issues that confront them in their professional lives. Experimental procedures and troubleshooting of data are emphasised throughout the book. The book integrates theory and practices to ensure students understand why and how each technique is used.
Author | : U. S. Singh |
Publisher | : CRC Press |
Total Pages | : 544 |
Release | : 2017-12-14 |
Genre | : Science |
ISBN | : 1351430211 |
Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.
Author | : Heather B. Miller |
Publisher | : Academic Press |
Total Pages | : 227 |
Release | : 2011-10-18 |
Genre | : Science |
ISBN | : 0123855454 |
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author | : P. Vidhyasekaran |
Publisher | : CRC Press |
Total Pages | : 452 |
Release | : 2002-11-15 |
Genre | : Science |
ISBN | : |
Chapter 1. Molecular Recognition Processes Between Plant and Bacterial Pathogens Physical Contact of Plant Cells is Necessary for Bacterial Recognition Molecules Responsible for Physical Contact Many Bacterial Pathogens Induce Necrosis on Hosts and Nonhosts Bacterial Pathogens Grow in Both Host and Nonhost Plants Bacterial Pathogens Induce Leakage of Nutrients in Both Host and Nonhost Plants Bacterial Genes Involved in Recognition of Hosts and Nonhosts Coregulation of hrp, avr and Other Pathogenicity Genes Transcription of Bacterial Pathogenicity Genes in Planta Plant-Derived Molecules May Be Involved in Induction of Bacterial Genes Some Plant Signals May Direct Synthesis of Elicitors Secretion of Elicitors From Bacterial Cells in Plants The Role of hrp and avr Genes in Early Recognition Process in Plant-Bacterial Pathogen Interactions Other Signal Molecules of Bacterial Pathogens The Signal Transduction System Systemic Signal Induction Is Cell Death Involved in Signal Transduction Pathway? How Pathogens Avoid or Overcome Host Defense Mechanisms Induced by the Signal Transduction System? Possible Role of Signal Transduction System in Evasion of Host Recognition by Phytopathogenic Bacteria During Pathogenesis Chapter 2. Host Defense Mechanisms: Cell Wall-the First Barrier and a Source of Defense Signal Molecules The First Barrier to Bacterial Infection in Plants Structure of the Plant Cell Wall Pectic Polysaccharides Cellulose Hemicellulos Cell Wall Proteins Bacterial Genes Encoding Extracellular Enzymes Bacterial Genes Regulating Production of Extracellular Enzymes Bacterial Genes Regulating Secretion of Extracellular Enzymes Secretion of Proteases The Signaling System in Induction of Bacterial Extracellular Enzymes Plant Cell Wall Components Involved in Defense Mechanisms Against Bacterial Pathogens Bacterial Extracellular Enzymes Induce Host Defense Mechanisms Pectic Fragments Induce Virulence Genes in Bacterial and Defense Genes in Plants Pectic Enzymes Vary in Inducing Resistance or Susceptibility Polygalacturonase-Inhibiting Proteins Cell Wall Modifications and Bacterial Disease Resistance Chapter 3. Active Oxygen Species Mechanism of Production of Active Oxygen Species Signals for Induction of Active Oxygen Species in Bacteria-Infected Plants Bacterial Infection Leads to Production of Active Oxygen Species in Plants Active Oxygen Species May Induce Lipid Peroxidation Increases in Active Oxygen Species Lead to Activation of Lipoxygenase Active Oxygen Species Production Leads to Cell Membrane Damage Active Oxygen Species May Directly Kill Bacterial Pathogens Bacterial Pathogens May Tolerate Toxicity of Active Oxygen Species Antioxidants of the Host May Protect Bacterial Pathogens Against Active Oxygen Species The Possible Role of Active Oxygen Species in Disease Resistance Chapter 4. Inducible Plant Proteins Nomenclature of Pathogen-Inducible Plant Proteins Occurrence of PR Proteins in Various Plants Classification of PR Proteins Bacterial Pathogens Induce PR Proteins Molecular Mechanisms of Induction of PR Proteins Compartmentalization of PR Proteins in Plant Tissues The Role of PR Proteins in Bacterial Disease Resistance The Second Group of Pathogen-Inducible Proteins: Constitutive, but Increasingly Induced Hydroxyproline-Rich Glycoproteins Lectins Not All Inducible Proteins Need Be Involved in Inducing Bacterial Disease Resistance Chapter 5. Inducible Secondary Metabolites What Are Inducible Secondary Metabolites? Bacterial Pathogens Induce Accumulation of Secondary Metabolites in Infected Tissues Phytoalexins Accumulate in Plants After Irreversible Cell Membrane Damage Phytoalexins Accumulate Only Locally and Not Systemically Mode of Syntheses of Phytoalexins Evidences That Induced Secondary Metabolites Are Involved in Bacterial Disease Resistance Phytoalexins May Be Suppressed, Degraded, or Inactivated in Susceptible Interactions Some Phytoalexins May Not Have Any Role in Disease Resistance Constitutive, but Induced Secondary Metabolites During Pathogenesis Chapter 6. Biotechnological Applications: Molecular Manipulation of Bacterial Disease Resistance Manipulation of Signal Transduction System for Induction of Disease Resistance Manipulation of Resistance Genes Involved in Signal Transduction System Manipulation of Signal Transduction System by Elicitors Manipulation of Signal Transduction System by Using Chemicals Manipulation of Signal Transduction System by Using Rhizobacterial Strains Manipulation of Signal Transduction System by Enhanced Biosynthesis of Salicylic Acid Manipulation of Signal Transduction System by Inducing Accelerated Cell Death Manipulation of Signal Transduction System by Enhanced Biosynthesis of Cytokinins Manipulation of Inducible Proteins for Induction of Bacterial Disease Resistance Suppression of Virulence Factors of Bacterial Pathogens to Manage Bacterial Diseases Exploitation of Insect Genes Encoding Antibacterial Proteins for Bacterial Disease Management Exploitation of Bacteriophage Genes for Bacterial Disease Management Exploitation of Genes from Human Beings, Hens, and Crabs for Management of Plant Bacterial Diseases References Index.
Author | : Khalid Z. Masoodi |
Publisher | : Academic Press |
Total Pages | : 220 |
Release | : 2020-10-28 |
Genre | : Science |
ISBN | : 012824450X |
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Author | : Vivek Sharma |
Publisher | : Academic Press |
Total Pages | : 454 |
Release | : 2020-03-12 |
Genre | : Technology & Engineering |
ISBN | : 0128184698 |
Molecular Aspects of Plant Beneficial Microbes in Agriculture explores their diverse interactions, including the pathogenic and symbiotic relationship which leads to either a decrease or increase in crop productivity. Focusing on these environmentally-friendly approaches, the book explores their potential in changing climatic conditions. It presents the exploration and regulation of beneficial microbes in offering sustainable and alternative solutions to the use of chemicals in agriculture. The beneficial microbes presented here are capable of contributing to nutrient balance, growth regulators, suppressing pathogens, orchestrating immune response and improving crop performance. The book also offers insights into the advancements in DNA technology and bioinformatic approaches which have provided in-depth knowledge about the molecular arsenal involved in mineral uptake, nitrogen fixation, growth promotion and biocontrol attributes.
Author | : Palmiro Poltronieri |
Publisher | : Woodhead Publishing |
Total Pages | : 354 |
Release | : 2015-01-27 |
Genre | : Science |
ISBN | : 0081000715 |
Applied plant genomics and biotechnology reviews the recent advancements in the post-genomic era, discussing how different varieties respond to abiotic and biotic stresses, investigating epigenetic modifications and epigenetic memory through analysis of DNA methylation states, applicative uses of RNA silencing and RNA interference in plant physiology and in experimental transgenics, and plants modified to produce high-value pharmaceutical proteins. The book provides an overview of research advances in application of RNA silencing and RNA interference, through Virus-based transient gene expression systems, Virus induced gene complementation (VIGC), Virus induced gene silencing (Sir VIGS, Mr VIGS) Virus-based microRNA silencing (VbMS) and Virus-based RNA mobility assays (VRMA); RNA based vaccines and expression of virus proteins or RNA, and virus-like particles in plants, the potential of virus vaccines and therapeutics, and exploring plants as factories for useful products and pharmaceuticals are topics wholly deepened. The book reviews and discuss Plant Functional Genomic studies discussing the technologies supporting the genetic improvement of plants and the production of plant varieties more resistant to biotic and abiotic stresses. Several important crops are analysed providing a glimpse on the most up-to-date methods and topics of investigation. The book presents a review on current state of GMO, the cisgenesis-derived plants and novel plant products devoid of transgene elements, discuss their regulation and the production of desired traits such as resistance to viruses and disease also in fruit trees and wood trees with long vegetative periods. Several chapters cover aspects of plant physiology related to plant improvement: cytokinin metabolism and hormone signaling pathways are discussed in barley; PARP-domain proteins involved in Stress-Induced Morphogenetic Response, regulation of NAD signaling and ROS dependent synthesis of anthocyanins. Apple allergen isoforms and the various content in different varieties are discussed and approaches to reduce their presence. Euphorbiaceae, castor bean, cassava and Jathropa are discussed at genomic structure, their diseases and viruses, and methods of transformation. Rice genomics and agricultural traits are discussed, and biotechnology for engineering and improve rice varieties. Mango topics are presented with an overview of molecular methods for variety differentiation, and aspects of fruit improvement by traditional and biotechnology methods. Oilseed rape is presented, discussing the genetic diversity, quality traits, genetic maps, genomic selection and comparative genomics for improvement of varieties. Tomato studies are presented, with an overview on the knowledge of the regulatory networks involved in flowering, methods applied to study the tomato genome-wide DNA methylation, its regulation by small RNAs, microRNA-dependent control of transcription factors expression, the development and ripening processes in tomato, genomic studies and fruit modelling to establish fleshy fruit traits of interest; the gene reprogramming during fruit ripening, and the ethylene dependent and independent DNA methylation changes. - provides an overview on the ongoing projects and activities in the field of applied biotechnology - includes examples of different crops and applications to be exploited - reviews and discusses Plant Functional Genomic studies and the future developments in the field - explores the new technologies supporting the genetic improvement of plants
Author | : Bob B. Buchanan |
Publisher | : John Wiley & Sons |
Total Pages | : 1280 |
Release | : 2015-08-31 |
Genre | : Science |
ISBN | : 0470714220 |
With over 1000 original drawings and 500 photographs, this work offers complete coverage of cell biology, plant physiology and molecular biology.
Author | : Heddwyn Jones |
Publisher | : |
Total Pages | : 466 |
Release | : 1995 |
Genre | : Science |
ISBN | : 9781592595365 |
In Plant Gene Transfer and Expression Protocols, leading experts present the latest step-by-step recipes for introducing genes into model and agricultural plants and for studying gene activity using reporter enzyme assays, RNA techniques, and immunological methods. The book contains not only a complete collection of mainstream methods, but also methods for studying gene expression and genetic manipulation in chloroplasts and mitochondria. There are also methods for introducing and analyzing plant genes in Xenopus and heterologous yeast hosts. Plant Gene Transfer and Expression Protocols is an indispensable tool for scientists working in both fundamental (biochemical, physiological, and genetic) and applied aspects of plant biotechnology, plant breeding, agriculture, and horticulture. It provides concise practical help that produces the desired results even for beginners and opens the doors to new research possibilities.
Author | : C. Neal Stewart, Jr. |
Publisher | : John Wiley & Sons |
Total Pages | : 554 |
Release | : 2012-12-13 |
Genre | : Science |
ISBN | : 1118589483 |
Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.