Molecular Biological Methods For Bacillus
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| Author | : Colin R. Harwood |
| Publisher | : Wiley |
| Total Pages | : 618 |
| Release | : 1991-02-18 |
| Genre | : Science |
| ISBN | : 9780471923930 |
Molecular Biological Methods for Bacillus Edited by C. R. Harwood, Department of Microbiology, University of Newcastle upon Tyne, UK and S. M. Cutting, The Biological Laboratories, Harvard University, USA This volume represents the first major attempt to produce a compendium of the experimental methods used for the analysis of Bacillus, one of the most important procaryotic genera. Since the pioneering work on the transformation and genetic analysis of Bacillus subtilis by John Spizizen and his colleagues in the late 1950s this microorganism has been extensively studied and is now one of the best understood. More than forty of the world’s leading researchers in the field have generously contributed experimental procedures—devised and used in their laboratories—to this book. The aim throughout has been to present methods as simple step-by-step protocols which have been thoroughly tried and tested. The context in which the methods are used is discussed in detail and relevant information provided on the physiology and genetics of Bacillus. In addition valuable support is provided in the form of troubleshooting tips and advice on safety, the preparation of reagents, and the use of equipment. The book will be invaluable to those working with the genus Bacillus and related genera—both established researchers and those wishing to use this important microorganism for the first time.
| Author | : Barbara H. Iglewski |
| Publisher | : Elsevier |
| Total Pages | : 486 |
| Release | : 1990-01-01 |
| Genre | : Science |
| ISBN | : 0323148670 |
Molecular Basis of Bacterial Pathogenesis focuses on the molecular mechanism of disease associated with bacterial pathogens. Topics covered include the population genetics of bacterial pathogenesis; environmental modulation of gene expression in gram-negative pathogens; and bacterial invasion and intracellular growth. Bacterial toxins are also discussed. This volume is comprised of 20 chapters and begins with an overview of pathogenesis, paying particular attention to common elements and genetic mechanisms of regulation. The discovery that many bacterial pathogens are clonal, with individual clones often having a greater virulence than others, is then considered. The next section deals with the regulation of synthesis of surface components and their role in colonization of the host and/or evasion of the host immune defense systems; antigenic variation and its role in evasion of the host immune response; and the role of iron acquisition systems in the colonization of the host. Subsequent chapters explore the invasion and intracellular growth of facultative and obligate intracellular parasites. The last section is devoted to studies on the role of bacterial toxic products in pathogenesis. Bacterial lipopolysaccharides (endotoxins) and exotoxins are described. This book should be of interest to molecular biologists, physiologists, clinical specialists, pathologists, and geneticists.
| Author | : Sang Yup Lee |
| Publisher | : John Wiley & Sons |
| Total Pages | : 1075 |
| Release | : 2021-06-02 |
| Genre | : Science |
| ISBN | : 352782345X |
Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering: Concepts and Applications delivers a one-stop resource for readers seeking a complete description of the concepts, models, and applications of metabolic engineering. This guide offers practical insights into the metabolic engineering of major cell lines, including E. Coli, Bacillus and Yarrowia Lipolytica, and organisms, including human, animal, and plant). The distinguished editors also offer readers resources on microbiome engineering and the use of metabolic engineering in bioremediation. Written in two parts, Metabolic Engineering begins with the essential models and strategies of the field, like Flux Balance Analysis, Quantitative Flux Analysis, and Proteome Constrained Models. It also provides an overview of topics like Pathway Design, Metabolomics, and Genome Editing of Bacteria and Eukarya. The second part contains insightful descriptions of the practical applications of metabolic engineering, including specific examples that shed light on the topics within. In addition to subjects like the metabolic engineering of animals, humans, and plants, you’ll learn more about: Metabolic engineering concepts and a historical perspective on their development The different modes of analysis, including flux balance analysis and quantitative flux analysis An illuminating and complete discussion of the thermodynamics of metabolic pathways The Genome architecture of E. coli, as well as genome editing of both bacteria and eukarya An in-depth treatment of the application of metabolic engineering techniques to organisms including corynebacterial, bacillus, and pseudomonas, and more Perfect for students of biotechnology, bioengineers, and biotechnologists, Metabolic Engineering: Concepts and Applications also has a place on the bookshelves of research institutes, biotechnological institutes and industry labs, and university libraries. It's comprehensive treatment of all relevant metabolic engineering concepts, models, and applications will be of use to practicing biotechnologists and bioengineers who wish to solidify their understanding of the field.
| Author | : |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2002 |
| Genre | : Cells |
| ISBN | : 9780815332183 |
| Author | : National Research Council |
| Publisher | : National Academies Press |
| Total Pages | : 254 |
| Release | : 2004-07-08 |
| Genre | : Science |
| ISBN | : 0309166152 |
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
| Author | : Keith Wilson |
| Publisher | : Cambridge University Press |
| Total Pages | : 769 |
| Release | : 2010-03-04 |
| Genre | : Medical |
| ISBN | : 0521516358 |
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
| Author | : World Health Organization |
| Publisher | : World Health Organization |
| Total Pages | : 219 |
| Release | : 2008 |
| Genre | : Business & Economics |
| ISBN | : 9241547537 |
This fourth edition of the anthrax guidelines encompasses a systematic review of the extensive new scientific literature and relevant publications up to end 2007 including all the new information that emerged in the 3-4 years after the anthrax letter events. This updated edition provides information on the disease and its importance, its etiology and ecology, and offers guidance on the detection, diagnostic, epidemiology, disinfection and decontamination, treatment and prophylaxis procedures, as well as control and surveillance processes for anthrax in humans and animals. With two rounds of a rigorous peer-review process, it is a relevant source of information for the management of anthrax in humans and animals.
| Author | : Christoph Wittmann |
| Publisher | : John Wiley & Sons |
| Total Pages | : 789 |
| Release | : 2017-04-10 |
| Genre | : Science |
| ISBN | : 352734179X |
The latest volume in the Advanced Biotechnology series provides an overview of the main production hosts and platform organisms used today as well as promising future cell factories in a two volume book. Alongside describing tools for genetic and metabolic engineering for strain improvement, the authors also impart topical information on computational tools, safety aspects and industrial-scale production. Following an introduction to general concepts, historical developments and future technologies, the text goes on to cover multi-purpose bacterial cell factories, including those organisms that exploit anaerobic biosynthetic power. Further chapters deal with microbes used for the production of high-value natural compounds and those obtained from alternative raw material sources, concluding with eukaryotic workhorses.
| Author | : Makoto Kanauchi |
| Publisher | : Humana |
| Total Pages | : 0 |
| Release | : 2024-10-05 |
| Genre | : Technology & Engineering |
| ISBN | : 9781071640951 |
This updated volume presents experimentation-based approaches to lactic acid bacteria (LAB) research. Split into three parts, the book explores techniques for analyzing lactic acid bacteria metabolism and characteristics, applications for food-related industries, such as yogurt production, beer, and wine making, and functions of LAB in human health. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Lactic Acid Bacteria: Methods and Protocols, Second Edition serves as an ideal guide for improving research into this vital area of nutrition and health science.
| Author | : Shmuel Razin |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 574 |
| Release | : 2007-05-08 |
| Genre | : Science |
| ISBN | : 0306476061 |
was the result of the efforts of Robert Cleverdon. The rapidly developing discipline of molecular biology and the rapidly expanding knowledge of the PPLO were brought together at this meeting. In addition to the PPLO specialists, the conference invited Julius Marmur to compare PPLO DNA to DNA of other organisms; David Garfinkel, who was one of the first to develop computer models of metabolism; Cyrus Levinthal to talk about coding; and Henry Quastler to discuss information theory constraints on very small cells. The conference was an announcement of the role of PPLO in the fundamental understanding of molecular biology. Looking back 40-some years to the Connecticut meeting, it was a rather bold enterprise. The meeting was international and inter-disciplinary and began a series of important collaborations with influences resonating down to the present. If I may be allowed a personal remark, it was where I first met Shmuel Razin, who has been a leading figure in the emerging mycoplasma research and a good friend. This present volume is in some ways the fulfillment of the promise of that early meeting. It is an example of the collaborative work of scientists in building an understanding of fundamental aspects of biology.
