Modulated Apertures And Resolution In Microscopy
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| Author | : Abdallah Mohamed Hamed |
| Publisher | : Springer Nature |
| Total Pages | : 106 |
| Release | : 2023-12-16 |
| Genre | : Science |
| ISBN | : 3031475526 |
This book serves as a valuable resource for researchers and graduate students specializing in optical engineering and optical sciences. Comprising three distinct parts, it addresses fundamental aspects and practical applications of modulated apertures in microscopy. The first part delves into the fabrication of modulated apertures and the computation of impulse responses or point spread functions (PSFs). It offers essential insights into the foundational concepts of modulated aperture design. The second part focuses on the utilization of modulated apertures in speckle imaging, elucidating their significance and relevance in this context. This section provides a comprehensive understanding of the practical applications of modulated apertures in image formation. The third and final part explores the application of modulated apertures within the framework of a confocal scanning laser microscope. The objective here is to enhance microscope resolution and image contrast, contributing to the advancement of microscopy techniques. This book offers a concise and objective exploration of modulated apertures' fabrication, applications, and their potential to enhance microscopy. This book is a valuable reference for students and researchers seeking to deepen their knowledge in this specialized field.
| Author | : Abdallah Hamed |
| Publisher | : Springer Nature |
| Total Pages | : 124 |
| Release | : |
| Genre | : |
| ISBN | : 3031583000 |
| Author | : Abdallah Mohamed Hamed |
| Publisher | : Springer |
| Total Pages | : 0 |
| Release | : 2023-12-27 |
| Genre | : Science |
| ISBN | : 9783031475511 |
This book serves as a valuable resource for researchers and graduate students specializing in optical engineering and optical sciences. Comprising three distinct parts, it addresses fundamental aspects and practical applications of modulated apertures in microscopy. The first part delves into the fabrication of modulated apertures and the computation of impulse responses or point spread functions (PSFs). It offers essential insights into the foundational concepts of modulated aperture design. The second part focuses on the utilization of modulated apertures in speckle imaging, elucidating their significance and relevance in this context. This section provides a comprehensive understanding of the practical applications of modulated apertures in image formation. The third and final part explores the application of modulated apertures within the framework of a confocal scanning laser microscope. The objective here is to enhance microscope resolution and image contrast, contributing to the advancement of microscopy techniques. This book offers a concise and objective exploration of modulated apertures' fabrication, applications, and their potential to enhance microscopy. This book is a valuable reference for students and researchers seeking to deepen their knowledge in this specialized field.
| Author | : Randy O. Wayne |
| Publisher | : Academic Press |
| Total Pages | : 373 |
| Release | : 2013-12-16 |
| Genre | : Science |
| ISBN | : 0124115365 |
The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. - Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope - Clearly develops all ideas from historical and logical foundations - Laboratory exercises included to assist the reader with practical applications - Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope
| Author | : Philip Tinnefeld |
| Publisher | : Springer |
| Total Pages | : 340 |
| Release | : 2015-02-07 |
| Genre | : Science |
| ISBN | : 3662455471 |
This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
| Author | : Bernhard Schaller |
| Publisher | : BoD – Books on Demand |
| Total Pages | : 404 |
| Release | : 2012-03-16 |
| Genre | : Medical |
| ISBN | : 9535103598 |
The present book gives an exceptional overview of molecular imaging. Practical approach represents the red thread through the whole book, covering at the same time detailed background information that goes very deep into molecular as well as cellular level. Ideas how molecular imaging will develop in the near future present a special delicacy. This should be of special interest as the contributors are members of leading research groups from all over the world.
| Author | : James Pawley |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 234 |
| Release | : 2012-12-06 |
| Genre | : Medical |
| ISBN | : 1461571332 |
In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.
| Author | : Pranab Dey |
| Publisher | : Springer Nature |
| Total Pages | : 350 |
| Release | : 2023-01-01 |
| Genre | : Medical |
| ISBN | : 9811966168 |
The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc. The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing. Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique. The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.
| Author | : Alan J. Lacey |
| Publisher | : OUP Oxford |
| Total Pages | : 475 |
| Release | : 1999-06-03 |
| Genre | : Science |
| ISBN | : 0191565725 |
Since the first edition of Light Microscopy in Biology: A Practical approach was published, techniques in modern light microscopy have improved considerably. This fully updated edition includes revised topics from the first edition as well as coverage of techniques and technologies that have been developed since it was published. As before, the book starts with an explanation of the basic techniques, and goes on to describe current methods in: chromosome microscopy, immunohistochemistry, fluorescence microscopy, image building and video microscopy. Totally new topics covered include: confocal microscopy, calcium and pH imaging, microinjection techniques and nanovid microscopy. There are also whole chapters now devoted to reflection contrast microscopy and histomorphometry. This new edition will be of great interest to postgraduate and postdoctoral researchers in biomedicine and cell biology - both those experienced with light microscopic techniques and newcomers to the field.
| Author | : Ulrich Kubitscheck |
| Publisher | : John Wiley & Sons |
| Total Pages | : 402 |
| Release | : 2013-04-02 |
| Genre | : Medical |
| ISBN | : 3527671617 |
A comprehensive introduction to advanced fluorescence microscopy methods and their applications. This is the first title on the topic designed specifically to allow students and researchers with little background in physics to understand both microscopy basics and novel light microscopy techniques. The book is written by renowned experts and pioneers in the field with a rather intuitive than formal approach. It always keeps the nonexpert reader in mind, making even unavoidable complex theoretical concepts readily accessible. All commonly used methods are covered. A companion website with additional references, examples and video material makes this a valuable teaching resource: http://www.wiley-vch.de/home/fluorescence_microscopy/
