Modern Size Exclusion Liquid Chromatography
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| Author | : Andre Striegel |
| Publisher | : John Wiley & Sons |
| Total Pages | : 512 |
| Release | : 2009-07-31 |
| Genre | : Science |
| ISBN | : 0470442832 |
The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheology Throughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.
| Author | : W. W. Yau |
| Publisher | : Wiley-Interscience |
| Total Pages | : 500 |
| Release | : 1979-09-27 |
| Genre | : Science |
| ISBN | : |
A critical treatment of all aspects, containing definitive examples, detailed description of data handling, compilations of information on columns and column packings, and tables of important solvents and standard reference materials. Combines a basic, unified theory with practical know-how, integrating methods of gel permeation and gel filtration chromatography. Provides an understanding of molecular characterization problems as well as a background for developing new and improved characterization methods and apparatus for HPSEC performed with columns of small particles at high pressures.
| Author | : Lloyd R. Snyder |
| Publisher | : John Wiley & Sons |
| Total Pages | : 831 |
| Release | : 2011-09-20 |
| Genre | : Science |
| ISBN | : 1118210395 |
The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.
| Author | : Lloyd R. Snyder |
| Publisher | : John Wiley & Sons |
| Total Pages | : 665 |
| Release | : 2012-12-03 |
| Genre | : Science |
| ISBN | : 1118591518 |
This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.
| Author | : B. J. Hunt |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 292 |
| Release | : 2013-04-17 |
| Genre | : Science |
| ISBN | : 1461578612 |
There is a large and increasing variety of polymers currently in use both for domestic and industrial applications. The properties of polymers are deter mined not only by their chemical type, but also by their molecular mass and molecular mass distributions. However, while the chemical type of polymers can be determined relatively easily, the average molecular masses and molecular mass distributions are more difficult to measure. The molecular mass averages of a polymer are measured by specialized and complex techniques such as light scattering (for weight average) and osmometry (for number average). Thus, complete characterization of the molecular mass distribution of a polymer by such means requires separating the sample into many fractions which can then be examined individually. Since size exclusion chromatography was introduced as a rapid and straightforward technique for the characterization of polymer molecular mass distributions, there have been tremendous increases in development and applications, and it was felt appropriate to bring together into a single volume the information required by scientists from many disciplines who wish to use the technique. This book should be useful to existing users, those who are new to the technique, and those who may be familiar with the basic technique and now wish to extend their capabilities to more complex applications (or to consider the potential of a number of related techniques). The book will also be of general interest to the experienced liquid chromatographer.
| Author | : Serban C. Moldoveanu |
| Publisher | : Elsevier |
| Total Pages | : 718 |
| Release | : 2022-06-24 |
| Genre | : Science |
| ISBN | : 0323998364 |
Essentials in Modern HPLC Separations, Second Edition discusses the role of separation in high performance liquid chromatography (HPLC). This new and updated edition systematically presents basic concepts as well as new developments in HPLC. Starting with a description of basic concepts, it provides important guidance for the practical utilization of various HPLC procedures, such as the selection of the HPLC type, proper choice of the chromatographic column, selection of mobile phase and selection of the method of detection, all of which are in correlation with the physico-chemical characteristics of the compounds separated. Every chapter has been carefully reviewed, with several new sections added to bring the book completely up-to-date. Hence, it is a valuable reference for students and professors in chemistry. - Provides a thoroughly updated resource, with an entirely new section on Computer-aided Method Development in HPLC and new subsections on miniaturization and automation in HPLC, chemometric aspects of HPLC, green solvent use in HPLC, and more - Includes insights into the chromatographic process to find the optimum solution for analyzing complex samples - Presents a basis for understanding the utilization of modern HPLC for applications, particularly for the analysis of pharmaceutical, biological, food, beverage and environmental samples
| Author | : André M. Striegel |
| Publisher | : |
| Total Pages | : 370 |
| Release | : 2005 |
| Genre | : Science |
| ISBN | : |
For four decades, size-exclusion chromatography has played a prominent role in characterizing polymers, by determining the polymer's molar mass averages, often not absolutely but relative to some calibration standard. Not satisfied, scientists now want to determine the absolute molar mass averages and distribution and to characterize their long-chain and short-chain branching, tacticity, copolymer and base-pair sequences, and other matters. Oh yes, and they want all this as a continuous function of the molar mass of the analyte. Fortunately, the technique is endowed with a number of detection methods, and the 18 papers here explain the role of those various detection methods and the synergistic effect of combining them in different configurations to obtain desired results. Distributed in the US by Oxford University Press. Annotation : 2004 Book News, Inc., Portland, OR (booknews.com).
| Author | : Ziad El Rassi |
| Publisher | : Elsevier |
| Total Pages | : 956 |
| Release | : 2021-08-15 |
| Genre | : Science |
| ISBN | : 0128245123 |
Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. - Covers the basic principles of modern liquid phase separation techniques, along with their applications - Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science - Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress
| Author | : Chi-San Wu |
| Publisher | : CRC Press |
| Total Pages | : 720 |
| Release | : 2003-11-04 |
| Genre | : Science |
| ISBN | : 9780203913321 |
Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner. This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC). It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.
| Author | : Michael W. Dong |
| Publisher | : John Wiley & Sons |
| Total Pages | : 285 |
| Release | : 2016-04-06 |
| Genre | : Science |
| ISBN | : 111929360X |
A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and "greener" methods Regulatory aspects While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.
