Methods Of Cell Separation
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Author | : P.T. Sharpe |
Publisher | : Elsevier |
Total Pages | : 287 |
Release | : 1988-05-01 |
Genre | : Science |
ISBN | : 0080858872 |
Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments. A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method. Methods of Cell Separation is distinguished by three powerful assets: descriptions of the majority of cell separation methods currently being used; details of the experimental procedures involved in each method; and comparisons of the different methods for separating cell populations with particular reference to blood lymphocytes. An excellent addition to a distinguished series, and extremely useful as a laboratory manual.
Author | : Nicholas Catsimpoolas |
Publisher | : Springer Science & Business Media |
Total Pages | : 370 |
Release | : 2012-12-06 |
Genre | : Medical |
ISBN | : 1468408208 |
Presently, the need for methods involving separation, identification, and characterization of different kinds of cells is amply realized among immu nologists, hematologists, cell biologists, clinical pathologists, and cancer researchers. Unless cells exhibiting different functions and stages of differ entiation are separated from one another, it will be exceedingly difficult to study some of the molecular mechanisms involved in cell recognition, specialization, interactions, cytotoxicity, and transformation. Clinical diag nosis of diseased states and use of isolated cells for therapeutic (e. g. , immunotherapy) or survival (e. g. , transfusion) purposes are some of the pressing areas where immediate practical benefits can be obtained by applying cell separation techniques. However. the development of such useful methods is still in its infancy. A number of good techniques exist based either on the physical or biological properties of the cells, and these have produced some valuable results. Still others are to be discovered. Therefore, the purpose of this open-end treatise is to acquaint the reader with some of the basic principles, instrumentation, and procedures pres ently in practice at various laboratories around the world and to present some typical applications of each technique to particular biological prob lems. To this end, I was fortunate to obtain the contribution of certain leading scientists in the field of cell separation, people who in their pioneer ing work have struggled with the particular problems involved in separating living cells and in some way have won.
Author | : Diether Recktenwald |
Publisher | : CRC Press |
Total Pages | : 354 |
Release | : 1997-11-04 |
Genre | : Medical |
ISBN | : 9780824798642 |
"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "
Author | : Thomas G. Pretlow |
Publisher | : Academic Press |
Total Pages | : 343 |
Release | : 2014-05-10 |
Genre | : Science |
ISBN | : 1483219380 |
Cell Separation: Methods and Selected Applications is a compendium of articles on the design and/or application of methods for the separation of cells. This volume presents contributions on relatively finite subjects on cell separation. It covers topics on cell separation such as methods for obtaining cells in suspension from animal tissues; some of the kinds of data that are helpful in the description of cell purifications; and separation of host cells infiltrating tumors and allografts by velocity sedimentation at unit gravity. The separation of different kinds of nucleated cells from blood by centrifugal elutriation; a new approach to the separation of cells at unit gravity; and the isolation and culture of homogeneous populations of glomerular cell types are elucidated as well. Experimental oncologists, hematologists, immunologists, cell biologists, endocrinologists, and others who are not already expert in the use of methods for cell separation will find the book highly useful.
Author | : Ashok Kumar |
Publisher | : Springer |
Total Pages | : 210 |
Release | : 2007-10-09 |
Genre | : Science |
ISBN | : 3540752633 |
With contributions by numerous experts
Author | : |
Publisher | : Elsevier |
Total Pages | : 473 |
Release | : 2011-08-31 |
Genre | : Science |
ISBN | : 0080553508 |
Cell separation is at the core of current methods in experimental biology and medicine. Its importance is illustrated by the large number of physical and biochemical principles that have been evaluated for application to cell separation. The development of cell separation methods is driven by the needs of biological and medical research, and the ever-increasing demands for sensitivity, selectivity, yield, timeliness and economy of the process. The interdisciplinary nature of research in this area and the volume of information available in research publications and conferences necessitates a basic description of the fundamental processes involved in magnetic cell separation that may help the user in navigating this wealth of information available online and in scientific publications. This book will appeal to researchers in many areas utilizing this technique, including those working in cell biology, clinical research, inorganic chemistry, biochemistry, chemical engineering, materials science, physics and electrical engineering. Provides examples of how to calculate the volume magnetic susceptibility, a fundamental quantity for calculating the magnetic force acting on a cell, from various types of magnetic susceptibilities available in literature Introduces the elements of magnetostatics as they apply to cell magnetization and the magnetization of magnetic micro- and nano- particles used for cell separation Describes the parameters used to determine cell magnetophoresis
Author | : A. M. Cheret |
Publisher | : |
Total Pages | : 400 |
Release | : 1982 |
Genre | : Cell separation |
ISBN | : |
Author | : Nicholas Catsimpoolas |
Publisher | : |
Total Pages | : 0 |
Release | : 1977 |
Genre | : Gardening |
ISBN | : 9781468408218 |
Author | : |
Publisher | : |
Total Pages | : 0 |
Release | : 2002 |
Genre | : Cells |
ISBN | : 9780815332183 |
Author | : Derek Fisher |
Publisher | : OUP Oxford |
Total Pages | : 290 |
Release | : 1998-11-26 |
Genre | : Science |
ISBN | : 0191565555 |
Techniques for separating cells are needed in many areas of cell biology. This book presents modern methods from the laboratories of experts in the field, and includes tested, reproducible protocols, hints and tips for success, and troubleshooting suggestions. It will be invaluable to a wide range of cell biologists.