Methods for Affinity-Based Separations of Enzymes and Proteins

Methods for Affinity-Based Separations of Enzymes and Proteins
Author: Munishwar N. Gupta
Publisher: Birkhäuser
Total Pages: 234
Release: 2013-12-01
Genre: Science
ISBN: 3034881274

One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.

Methods for Affinity-based Separations of Enzymes and Proteins

Methods for Affinity-based Separations of Enzymes and Proteins
Author: Munishwar Nath Gupta
Publisher: Birkhauser
Total Pages: 246
Release: 2002
Genre: Science
ISBN:

One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.

Affinity Membranes

Affinity Membranes
Author: Elias Klein
Publisher: Wiley-Interscience
Total Pages: 176
Release: 1991-02-14
Genre: Science
ISBN:

Explores the latest findings for both selective and efficient separation devices in the field of kidney research. It is divided into three major sections. Part one deals with the ``biochemistry'' part of the problem, including how to identify ligands of interest, how to link them to synthetic membranes, and some kinetic limitations of frontal elution chromatography. The second part comprehensively discusses the various substrata used in affinity separations and the formation processes of semi-permeable membranes. The final section explores the filtration processes using membranes and the kinetics of separations based on affinity membranes.

Encyclopedia of Chromatography 2004 Update Supplement

Encyclopedia of Chromatography 2004 Update Supplement
Author: Jack Cazes
Publisher: CRC Press
Total Pages: 602
Release: 2004-08-11
Genre: Science
ISBN: 0824721535

In step with novel technologies and methodologies that have reshaped chromatography in recent years, this supplement reviews developments in HPLC, TLC, SFC, CCC, and other areas-presenting 50 authoritative entries filled with practical information vital to applications from biotechnology to environmental science to clinical pathology.

Food Biochemistry and Food Processing

Food Biochemistry and Food Processing
Author: Benjamin K. Simpson
Publisher: John Wiley & Sons
Total Pages: 909
Release: 2012-04-11
Genre: Technology & Engineering
ISBN: 1118308050

The biochemistry of food is the foundation on which the research and development advances in food biotechnology are built. In Food Biochemistry and Food Processing, Second Edition, the editors have brought together more than fifty acclaimed academicians and industry professionals from around the world to create this fully revised and updated edition. This book is an indispensable reference and text on food biochemistry and the ever increasing developments in the biotechnology of food processing. Beginning with sections on the essential principles of food biochemistry, enzymology, and food processing, the book then takes the reader on commodity-by-commodity discussions of biochemistry of raw materials and product processing. Chapters in this second edition have been revised to include safety considerations and the chemical changes induced by processing in the biomolecules of the selected foodstuffs. This edition also includes a new section on health and functional foods, as well as ten new chapters including those on thermally and minimally processed foods, separation technology in food processing, and food allergens. Food Biochemistry and Food Processing, second edition fully develops and explains the biochemical aspects of food processing, and brings together timely and relevant topics in food science and technology in one package. This book is an invaluable reference tool for professional food scientists, researchers and technologists in the food industry, as well as faculty and students in food science, food technology and food engineering programs. The Editor Dr. Benjamin K. Simpson, Department of Food Science and Agricultural Chemistry, McGill University, Quebec, Canada Associate Editors Professor Leo Nollet, Department of Applied Engineering Sciences, Hogeschool Ghent, Belgium Professor Fidel Toldrá, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Valencia, Spain Professor Soottawat Benjakul, Department of Food Technology, Prince of Songkla University, Songkhla, Thailand Professor Gopinadhan Paliyath, Department of Plant Agriculture, University of Guelph, Ontario, Canada Dr. Y. H. Hui, Consultant to the Food Industry, West Sacramento, California, USA

Antibodies

Antibodies
Author: G. Subramanian
Publisher: Springer Science & Business Media
Total Pages: 260
Release: 2013-03-07
Genre: Medical
ISBN: 1441988750

If the antibody industry is to achieve its full potential in the next decade, the individual technical potentials must be exploited, the limitations must be addressed, and lessons learned must be applied both to current purification methods and to the new technologies that continue to emerge. This book presents an overview of the current advances applied in the manufacture of monoclonal antibody including: -concepts in development of manufacturing strategies, -importance of antibody fragments, -application of chromatography method development, -quality control, -effect of expression on antibody properties, -virus removal and safety, -pharmacokinetics, -regulatory aspects.

Biocatalysis for Practitioners

Biocatalysis for Practitioners
Author: Gonzalo de Gonzalo
Publisher: John Wiley & Sons
Total Pages: 54
Release: 2021-07-19
Genre: Science
ISBN: 352734683X

This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.

Handbook of Affinity Chromatography

Handbook of Affinity Chromatography
Author: Toni Kline
Publisher: CRC Press
Total Pages: 1198
Release: 1993-06-16
Genre: Science
ISBN: 1482285010

Outlining the fundamental principles by which all interactions occur, this reference focuses on harnessing the biochemistry of bioorganic compounds in order to separate them, presenting new techniques and applications that affect the planning of research strategies. The contributors discuss how to c

Liquid Chromatography

Liquid Chromatography
Author: Salvatore Fanali
Publisher: Elsevier
Total Pages: 840
Release: 2017-06-23
Genre: Science
ISBN: 0128093447

Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. - Emphasizes the integration of chromatographic methods and sample preparation - Explains how liquid chromatography is used in different industrial sectors - Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) - Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making