Methods And Techniques In Plant Physiology
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| Author | : Cornelio Losa |
| Publisher | : |
| Total Pages | : 348 |
| Release | : 2016-08-01 |
| Genre | : |
| ISBN | : 9781681175430 |
Plants are loved by lots of people - in our homes, on our tables as foods, and in hundreds of products we use every day. Plants have many different usages. But how do plants develop from seeds, and how do they grow? This is where plant physiology comes into play. Plant physiology is the study of how different parts of plants function. It includes many aspects of plant life, including nutrition, movement, and growth. Fundamental processes such as photosynthesis, respiration, plant nutrition, plant hormone functions, tropisms, nastic movements, photoperiodism, photomorphogenesis, circadian rhythms, environmental stress physiology, seed germination, dormancy and stomata function and transpiration, both parts of plant water relations, are studied by plant physiologists. Plant physiology includes the study of biological and chemical processes of individual plant cells. Plant cells have a number of features that distinguish them from cells of animals, and which lead to major differences in the way that plant life behaves and responds differently from animal life. This book explores how plant physiology helps us to understand the many functions and behaviors of plants. Methods and Techniques in Plant Physiology is dedicated to physiology, biochemistry, cellular and molecular biology, genetics, biophysics, and environmental biology of plants. Techniques related to various physiological phenomenon are focus of tremendous interest and importance to plant physiologist, agronomist, horticulturist, ecologist, and biochemists.
| Author | : A. Bhattacharya |
| Publisher | : New India Publishing Agency |
| Total Pages | : 8 |
| Release | : 2015-01-01 |
| Genre | : Medical |
| ISBN | : 9383305509 |
Techniques related to various physiological phenomenon are subject of tremendous interest and importance to plant physiologist, agronomist, horticulturist, ecologist, and biochemists. This book is intended to provide recognized methods related various plant processes in a comprehensive form. Techniques on crop physiology such as hydroponics and plant nutrition, test for various stresses, water potential and water flow in plants, canopy gas measurements (Photosynthesis, Respiration and Transpiration), basic equations for growth studies and methods for estimations of plant products, microclimate. Efforts were also made to incorporate the topic like Climate Change and theory of phytotron as well as rhizotron in this book. The book will make the reader familiar with latest procedure to elucidate the problems. The validity of the results based on fundamentals principles of physics. This book is meant to be used in conjunction with a standard text of plant physiology though elementary principles relating to the techniques are briefed. The subjects on hormones, tissue culture and seed technology are useful for students. Hope this book shall serve the need of students, teachers and researchers.
| Author | : M. J. Reigosa Roger |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 450 |
| Release | : 2007-05-08 |
| Genre | : Science |
| ISBN | : 0306480573 |
The Handbook of Plant Ecophysiology Techniques you have now in your hands is the result of several combined events and efforts. The birth of this handbook can be traced as far as 1997, when our Plant Ecophysiology lab at the University of Vigo hosted a practical course on Plant Ecophysiology Techniques. That course showed us how much useful a handbook presenting a bunch of techniques would be for the scientists beginning to work on Plant Ecophysiology. In fact, we wrote a short handbook explaining the basics of the techniques taught in that 1997 course: Flow cytometry to measure ploidy levels, Use of a Steady-State porometer to measure transpiration, In vivo measure of fluorescence, HPLC analysis of low molecular weight phenolics, Spectrophotometric determinations of free proline and soluble proteins, TLC polyamines contents measures, Isoenzymatic electrophoresis, Use of IRGA and oxygen electrode. That modest handbook, written in Spanish, was very helpful, both for the people who attended the course and for other who have used it for beginning to work in Plant Ecophysiology. The present Handbook is much more ambitious, and it includes more techniques. But we have also had in mind the young scientists beginning to work on Plant Ecophysiology. In 1999 François Pellissier leaded a proposal presented to the European Commission in the Fifth Framework Program in the High Level * Scientific Conferences, including three EuroLab Courses about lab and field techniques useful to improve allelopathic research.
| Author | : Alexander G. Volkov |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 513 |
| Release | : 2007-04-19 |
| Genre | : Science |
| ISBN | : 354037843X |
This book compiles new findings in plant electrophysiology from the work of internationally renowned experts in the fields of electrophysiology, bio-electrochemistry, biophysics, signal transduction, phloem transport, tropisms, ion channels, plant electrochemistry, and membrane transport. Opening with a historical introduction, the book reviews methods in plant electrophysiology, introducing such topics as measuring membrane potentials and ion fluxes, path-clamp technique, and electrochemical sensors. The coverage includes experimental results and their theoretical interpretation.
| Author | : Adela M. Sánchez-Moreiras |
| Publisher | : Springer |
| Total Pages | : 491 |
| Release | : 2018-08-17 |
| Genre | : Science |
| ISBN | : 3319932330 |
This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology.
| Author | : Robert W. Pearcy |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 463 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 9401090130 |
capable of providing at least a relative measure of stomatal aperture were first used shortly thereafter (Darwin and Pertz, 1911). The Carnegie Institution of Washington's Desert Research Laboratory in Tucson from 1905 to 1927 was the first effort by plant physiologists and ecologists to conduct team research on the water relations of desert plants. Measurements by Stocker in the North African deserts and Indonesia (Stocker, 1928, 1935) and by Lundegardh (1922) in forest understories were pioneering attempts to understand the environmental controls on photosynthesis in the field. While these early physiological ecologists were keen observers and often posed hypotheses still relevant today they were strongly limited by the methods and technologies available to them. Their measurements provided only rough approximations of the actual plant responses. The available laboratory equip ment was either unsuited or much more difficult to operate under field than laboratory conditions. Laboratory physiologists distrusted the results and ecologists were largely not persuaded of its relevance. Consequently, it was not until the 1950s and 1960s that physiological ecology began its current resurgence. While the reasons for this are complicated, the development and application of more sophisticated instruments such as the infrared gas analyzer played a major role. In addition, the development of micrometeorology led to new methods of characterizing the plant environments.
| Author | : Mary A. Schuler |
| Publisher | : Academic Press |
| Total Pages | : 188 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323137652 |
Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. - Eight basic experiments which can be used collectively or individually cover - Recombinant Cloning and Screening in E. coli; DNA Sequencing - Plant RNA Isolation and in Vitro Translations - Plant DNA Isolations and Genomic DNA Southern AnalysisChloroplast Isolation and Protein SynthesisPlant Tissue Culture and Agrobacterium Transformations - Experiments that have been student tested for three years - Blueprints for setting up gel rigs - Comprehensive course schedule outlining individual procedures to be finished in each lab segment - Course can be tailored to suit the needs of the individual instructor
| Author | : Parvaiz Ahmad |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 386 |
| Release | : 2013-11-12 |
| Genre | : Science |
| ISBN | : 1461485916 |
The global population is growing at an alarming rate and is anticipated to reach about 9.6 billion by the end of 2050. Addressing the problem of food scarcity for budding population vis-à-vis environmental changes is the main challenge plant biologists face in the contemporary era. Plant growth and productivity are scarce in many areas of the world due to a wide range of environmental stresses. The productive land is dwindling progressively by various natural and anthropogenic means that lead to enormous crop losses worldwide. Plants often experience these stresses and have the ability to withstand them. However, when the stress exceeds the normal tolerance level, plants accumulate organic osmolytes, osmoprotectants, cryoprotectants and antioxidant enzymes, which helps them tolerate these stresses and assist in their acclimatization towards the particular ambiance needed for maintaining their growth and development. Physiological Mechanisms and Adaptation Strategies in Plants Under Changing Environment, Volume 1 discuss drought and temperature stresses and their mitigation through different means. This volume illuminates how plants that are bombarded by diverse and changing environmental stimuli, undergo appropriate physiological alterations that enable their survival. The information covered in the book is also useful in building apposite strategies to counter abiotic and biotic stresses in plants. Written by a diverse group of internationally renowned scholars, Physiological Mechanisms and Adaptation Strategies in Plants Under Changing Environment, Volume 1 is a concise yet comprehensive resource that will be beneficial for the researchers, students, environmentalists and soil scientists of this field.
| Author | : P.J. Lumsden |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 439 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 9401107904 |
Over recent years, progress in micropropagation has not been as rapid as many expected and, even now, relatively few crops are produced commercially. One reason for this is that the biology of material growing in vitro has been insufficiently understood for modifications to standard methods to be made based on sound physiological principles. However, during the past decade, tissue culture companies and others have invested considerable effort to reduce the empirical nature of the production process. The idea of the conference `Physiology, Growth and Development of Plants and Cells in Culture' (Lancaster, 1992) was to introduce specialists in different areas of plant physiology to micropropagators, with the express aims of disseminating as wide a range of information to as large a number of participants as possible, and beginning new discussions on the constraints and potentials affecting the development of in vitro plant production methods. This book is based on presentations from the conference and has been divided into two main sections, dealing with either aspects of the in vitro environment -- light, nutrients, water, gas -- or with applied aspects of the culture process -- morphogenesis, acclimation, rejuvenation, contamination.
| Author | : P. Sudhakar |
| Publisher | : Academic Press |
| Total Pages | : 196 |
| Release | : 2016-04-05 |
| Genre | : Technology & Engineering |
| ISBN | : 0128041102 |
Phenotyping Crop Plants for Physiological and Biochemical Traits presents a proven range of methodologies and practices for effective, efficient, and appropriate typing of crop plants. By addressing the basic principles and precautions needed when conducting crop-based experiments, this book guides the reader in selecting the appropriate method based on the growing environment, whether greenhouse, pot, field, or liquid (hydroponic). By addressing the quantification of seed traits related to growth experiments, including their viability and vigor, this book presents methodology options for optimum yield based on potential abiotic stresses. - Discusses various methods that can contribute to phenotyping of crop plants for various physiological and biochemical traits - Presents reliable techniques for phenotyping or quantifying plant characters during varied climatic conditions - Provides insights for selecting appropriate methodologies for specific crop growing situations - Identifies the most appropriate protocols and methods for analyzing crop traits
