Manual Of Antisense Methodology
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| Author | : Gunther Hartmann |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 208 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 146155067X |
In the past few years, antisense methodology has moved from in vitro studies to in vivo studies and first human trials. While the basic concept of antisense technology is simple, the methodological problems associated with its use are numerous and complex. Antisense- based methods have proven to be a field of research where careful attention to experimental protocols and appropriate controls is necessary. The Manual of Antisense Methodology emphasizes the application of antisense oligonucleotides, and is a guide for the identification of antisense and non-antisense effects in different experimental settings. The work is organized into three sections: antisense application in vitro, antisense application in vivo (animal models) and finally, clinical antisense studies. Where at all possible, the methods are described in sufficient detail to allow reproduction of a given experiment. The Manual of Antisense Methodology will be of interest to researchers in immunology, cancer research, pharmacology and internal medicine; and physicians conducting clinical studies in these fields.
| Author | : |
| Publisher | : Elsevier |
| Total Pages | : 614 |
| Release | : 1999-11-01 |
| Genre | : Science |
| ISBN | : 0080496709 |
Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedical-oriented research.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
| Author | : Satinder Ahuja |
| Publisher | : Elsevier |
| Total Pages | : 740 |
| Release | : 2000-06-23 |
| Genre | : Technology & Engineering |
| ISBN | : 0080507794 |
It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories.Key Features* Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies* Encompasses both analytical and process-scale methods* Elucidates the importance of engineering process control* Details selection of plant and process equipment* Addresses economic considerations* Discusses future developments
| Author | : Stanley T. Crooke |
| Publisher | : CRC Press |
| Total Pages | : 2000 |
| Release | : 2001-09-14 |
| Genre | : Medical |
| ISBN | : 9780824705664 |
This state-of-the-art reference provides comprehensive coverage of the development of antisense oligonucleotides to inhibit cancer cells as well as those involved in infectious, inflammatory, and immune-mediated diseases-highlighting new tools and technologies in medicinal chemistry, RNA biochemistry, and molecular and cellular biology to produce new therapeutic compounds. Presents previously unpublished data on the use of antisense technology to dissect pharmacological processes and confirm the roles of various genes! Showcasing the benefits of antisense drug use, including reduced toxicity and earlier disease detection, Antisense Drug Technology discusses novel formulations of antisense drugs practical methods to design effective isotype selective inhibitors molecular mechanisms of antisense drugs mRNA as a current biological template modern postreceptor binding mechanisms and more! With contributions by over 60 seasoned experts in the field and containing more than 3000 helpful references, tables, drawings, and photographs, Antisense Drug Technology is an illuminating source for organic, medicinal, and pharmaceutical chemists; biochemists; geneticists; hematologists; oncologists; molecular and cell biologists; virologists; immunologists; and medical school and graduate students in these disciplines.
| Author | : United States. Patent and Trademark Office |
| Publisher | : |
| Total Pages | : 718 |
| Release | : 1998 |
| Genre | : Patent laws and legislation |
| ISBN | : |
| Author | : United States. Patent and Trademark Office |
| Publisher | : |
| Total Pages | : 1046 |
| Release | : |
| Genre | : Patent laws and legislation |
| ISBN | : |
| Author | : Roger L. Lundblad |
| Publisher | : CRC Press |
| Total Pages | : 422 |
| Release | : 2016-04-19 |
| Genre | : Medical |
| ISBN | : 1439849005 |
Examining the chemical modification of biological polymers and the emerging applications of this technology, Chemical Modification of Biological Polymers reflects the change in emphasis in this subsection of biotechnology from the study of protein structure and function toward applications in therapeutics and diagnostics. HighlightsThe basic organi
| Author | : George Studzinski |
| Publisher | : OUP Oxford |
| Total Pages | : 338 |
| Release | : 1999-11-25 |
| Genre | : Science |
| ISBN | : 0191565954 |
There are three main themes running through this volume. First, basic methods for measurement of cell proliferation are introduced and explained with reference to various systems, primarily in vitro, but in vivo procedures are also illustrated. The second theme is growth signalling, and is exemplified by methods for the analysis of transduction pathways for growth, beginning at the cell membrane and leading to the cell nucleus. The last theme presented here is growth cessation, illustrated by several systems for induction of cell differentiation, and of cell senescence. The emphasis throughout the book is on human cell systems, making it particularly relevant to scientists interested in human disease, especially cancer. Importantly, well proved methods for studying cell growth are supplemented by some novel approaches, e.g., studies of cell cycle checkpoints, cell spheroids, and nuclear architecture. Only two chapters have been retained, in an updated form from Cell Growth and Apoptosis, the predecessor volume. The book is written by a team of scientists highly experienced in procedures they describe, and offer details and hints found valuable in their own laboratories; thus, variants of the same general methods can be found in different chapters. These should be helpful to beginning as well as experienced investigators, and are designed to stimulate new approaches to old and new questions.
| Author | : Yury E. Khudyakov |
| Publisher | : CRC Press |
| Total Pages | : 435 |
| Release | : 2002-09-25 |
| Genre | : Medical |
| ISBN | : 1420040162 |
Combining elements of biochemistry, molecular biology, and immunology, artificial DNA can be employed in a number of scientific disciplines. Some of the varied applications include site-specific mutagenesis, hybridization, amplification, protein engineering, anti-sense technology, DNA vaccines, protein vaccines, recombinant antibodies, screening fo
| Author | : Piet Herdewijn |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 436 |
| Release | : 2008-02-04 |
| Genre | : Medical |
| ISBN | : 1592598234 |
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
