Luciferases And Fluorescent Proteins
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| Author | : Marlene A. Deluca |
| Publisher | : Elsevier |
| Total Pages | : 813 |
| Release | : 2013-09-11 |
| Genre | : Science |
| ISBN | : 1483273733 |
Bioluminescence and Chemiluminescence: Basic Chemistry and Analytical Applications is a compendium of papers presented at the second International Symposium on Analytical Applications of Bioluminescene and Chemiluminescence in San Diego, California on August 26-28, 1980. Part I deals with chemilunescence and excited states including topics on the spectroscopy of the solvent cage — generation and characteristics of the excited state and the three features of chemi- and bioluminescence. Part II deals with events prior to producing luminol-dependent chemiluminescence; this part also examines the effects of stimulants on membrane potential. Part III discusses bacterial bioluminescence and analyzes the properties of a lumazine protein from a bioluminescent bacterium. This part also analyzes accessory enzymes responsible for such bacterial bioluminescence. Part IV examines the chemistry of firefly bioluminescence and presents the formula of three reactions catalyzed by firefly luciferase. Part V analyzes bioluminescence from other sources such as the earthworm and land snails. Part VI discusses the applications of bioluminescence in clinical chemistry, soil science, and marine biology. Part VII deals with the future uses of bioluminescence, while Part VIII is an abstract of other papers dealing with this subject. This collection can be helpful for biologists, zoologists, micro-biologists, marine biologists, and researchers dealing with bio-chemistry and related disciplines.
| Author | : Martin Chalfie |
| Publisher | : John Wiley & Sons |
| Total Pages | : 486 |
| Release | : 2005-11-18 |
| Genre | : Science |
| ISBN | : 0471739480 |
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
| Author | : Osamu Shimomura |
| Publisher | : World Scientific |
| Total Pages | : 557 |
| Release | : 2019-06-06 |
| Genre | : Science |
| ISBN | : 9813277122 |
This book is the bible of bioluminescence and a must-read not only for the students but for those who work in various fields relating to bioluminescence. It summarizes current structural information on all known bioluminescent systems in nature, from well-studied ones to those that have been seldom investigated.This book remains an important source of chemical knowledge on bioluminescence and, since the second edition's publication in 2012, has been revised to include major developments in two systems: earthworm Fridericia and higher fungi whose luciferins have been elucidated and synthesized. These two new luciferins represent an essential addition to seven previously known, with fully rewritten sections covering this new subject matter.
| Author | : Vincent Pieribone |
| Publisher | : Harvard University Press |
| Total Pages | : 306 |
| Release | : 2005 |
| Genre | : Nature |
| ISBN | : 9780674019218 |
The discovery of green fluorescent protein revolutionized molecular biology, transforming our study of everything from the AIDS virus to the workings of the brain.
| Author | : Sylvia Daunert |
| Publisher | : John Wiley & Sons |
| Total Pages | : 256 |
| Release | : 2006-12-13 |
| Genre | : Science |
| ISBN | : 3527608699 |
The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.
| Author | : Lubov Brovko |
| Publisher | : SPIE-International Society for Optical Engineering |
| Total Pages | : 148 |
| Release | : 2010 |
| Genre | : Science |
| ISBN | : 9780819482471 |
Bioluminescence methods are gaining increased attention due to their sensitivity, selectivity, and simplicity, along with the fact that bioluminescence can be monitored bothin vitroandin vivo. This book introduces bioluminescence and fluorescence systems, along with the principles of their application forin vivoimaging of intracellular processes, and covers recent developments in optical (bioluminescence and fluorescence) imaging in cell biology. This book is intended for scientists and students involved in basic cell physiology research, as well as industry professionals, engineers, and managers involved in drug discovery and pre-clinical drug development. It discusses the practical aspects of luminescencein vivoimaging for monitoring intracellular processes. While some basic knowledge of biochemistry and biophysics is preferable, the book includes a brief review of fundamental principles to allow those not familiar with these disciplines to grasp basic concepts.
| Author | : Juliusz Ashot Kozak |
| Publisher | : CRC Press |
| Total Pages | : 343 |
| Release | : 2017-07-14 |
| Genre | : Science |
| ISBN | : 149875273X |
Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.
| Author | : Manfred Schwab |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 3307 |
| Release | : 2008-09-23 |
| Genre | : Medical |
| ISBN | : 3540368477 |
This comprehensive encyclopedic reference provides rapid access to focused information on topics of cancer research for clinicians, research scientists and advanced students. Given the overwhelming success of the first edition, which appeared in 2001, and fast development in the different fields of cancer research, it has been decided to publish a second fully revised and expanded edition. With an A-Z format of over 7,000 entries, more than 1,000 contributing authors provide a complete reference to cancer. The merging of different basic and clinical scientific disciplines towards the common goal of fighting cancer makes such a comprehensive reference source all the more timely.
| Author | : Kevin F. Sullivan |
| Publisher | : Elsevier |
| Total Pages | : 613 |
| Release | : 2007-12-14 |
| Genre | : Science |
| ISBN | : 0080557244 |
This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.
| Author | : John G. Burr |
| Publisher | : CRC Press |
| Total Pages | : 632 |
| Release | : 2020-08-19 |
| Genre | : Science |
| ISBN | : 1000146561 |
This book focuses on instrumentation of chemi- and bioluminescence and discusses the nature of chemiluminescence as the exothermic oxidation of a substrate organic compound to give an energy-rich product that is luminescent. It describes the applications of chemiluminescence.
