Lipid Modification by Enzymes and Engineered Microbes

Lipid Modification by Enzymes and Engineered Microbes
Author: Uwe T. Bornscheuer
Publisher: Elsevier
Total Pages: 396
Release: 2018-03-27
Genre: Technology & Engineering
ISBN: 0128131683

Lipid Modification by Enzymes and Engineered Microbes covers the state-of-the art use of enzymes as natural biocatalysts to modify oils, also presenting how microorganisms, such as yeast, can be designed. In the past ten years, the field has made enormous progress, not only with respect to the tools developed for the development of designer enzymes, but also in the metabolic engineering of microbes, the discovery of novel enzyme activities, and in reaction engineering/process development. For the first time, these advances are covered in a single-volume that is edited by leading enzymatic scientist Uwe Borchscheuer and authored by an international team of experts. Identifies how, and when, to use enzymes and microbes for lipid modification Provides enzymatic, microbial and metabolic techniques for lipid modification Covers lipases, acyltransferases, phospholipases, lipoxygenases, monooxygenases, isomerases and sophorolipids Includes lipid modification for use in food, biofuels, oleochemicals and polymer precursors

Lipid Biotechnology

Lipid Biotechnology
Author: Tsung Min Kuo
Publisher: CRC Press
Total Pages: 837
Release: 2002-01-22
Genre: Science
ISBN: 0824744187

This text presents the latest advances in supercritical fluid technology, biocatalysis, bioprocess engineering, and crop breeding. It offers an in-depth review of the most recent principles and approaches utilized in the development and design of lipids for cosmetic, industrial and pharmaceutical, and food products. Discussing a variety of lipid-active enzymes from animal, plant, fungal, and microbial sources, "Lipid Biotechnology" covers modern techniques in genetic engineering for the modification of conventional oilseed crops and biosynthetic pathways for cutin polymers, flavor volatiles, oxylipins, and terpenoid compounds. It chronicles the use of lipases and phospholipases in the creation of structured lipids and fats, including cocoa butter, low-calorie fats, and Betapol, and emerging methods using supercritical carbon dioxide as a benign solvent for lipid analysis, fractionation, and enzymatic reaction. It also covers reaction conditions, reactor design, solvent selection, immobilization technology, and enzyme sources for optiml large-scale manufacturing, and describes the formation of oxylipins through the lipoxygenase pathway, as well as other unusual fatty acids. The authors provide in-depth analyses of the structure, metabolic and enzymatic functions and mechanisms, defensive and catalytic properties, industrial uses, and other applications of oxilipins and lipases.

Enzymes in Lipid Modification

Enzymes in Lipid Modification
Author: Uwe Theo Bornscheuer
Publisher: Wiley-Blackwell
Total Pages: 456
Release: 2000-09-13
Genre: Science
ISBN:

Enzymatic methods of lipid modification, particularly of fats and oils, have developed rapidly since the 1980s. In parallel to the rapid progress in research a wide range of applications have emerged, e.g. in the food industry. The book is written by leading experts in the field and reflects the state-of-the-art of enzymatic lipid modification. It provides the reader with guidelines how to select suitable enzymes and how to apply them efficiently. Applications of lipases and phospholipases, lipoxygenases and P450-monooxygenases and the use of whole-cell systems in lipid modification are described. Cloning, expression and mutagenesis as well as attempts to understand the molecular basis of specificity and stereoselectivity are outlined. In addition engineering aspects and the choice of solvent systems are addressed.

Lipid Biotechnology

Lipid Biotechnology
Author: Tsung Min Kuo
Publisher: CRC Press
Total Pages: 728
Release: 2002-01-22
Genre: Science
ISBN: 9780824706197

This text presents the latest advances in supercritical fluid technology, biocatalysis, bioprocess engineering, and crop breeding. It offers an in-depth review of the most recent principles and approaches utilized in the development and design of lipids for cosmetic, industrial and pharmaceutical, and food products. Discussing a variety of lipid-active enzymes from animal, plant, fungal, and microbial sources, "Lipid Biotechnology" covers modern techniques in genetic engineering for the modification of conventional oilseed crops and biosynthetic pathways for cutin polymers, flavor volatiles, oxylipins, and terpenoid compounds. It chronicles the use of lipases and phospholipases in the creation of structured lipids and fats, including cocoa butter, low-calorie fats, and Betapol, and emerging methods using supercritical carbon dioxide as a benign solvent for lipid analysis, fractionation, and enzymatic reaction. It also covers reaction conditions, reactor design, solvent selection, immobilization technology, and enzyme sources for optiml large-scale manufacturing, and describes the formation of oxylipins through the lipoxygenase pathway, as well as other unusual fatty acids. The authors provide in-depth analyses of the structure, metabolic and enzymatic functions and mechanisms, defensive and catalytic properties, industrial uses, and other applications of oxilipins and lipases.

Lipid Biotechnology

Lipid Biotechnology
Author: Tsung Min Kuo
Publisher: CRC Press
Total Pages: 728
Release: 2002-01-22
Genre: Science
ISBN: 9781135560485

This text presents the latest advances in supercritical fluid technology, biocatalysis, bioprocess engineering, and crop breeding. It offers an in-depth review of the most recent principles and approaches utilized in the development and design of lipids for cosmetic, industrial and pharmaceutical, and food products. Discussing a variety of lipid-active enzymes from animal, plant, fungal, and microbial sources, "Lipid Biotechnology" covers modern techniques in genetic engineering for the modification of conventional oilseed crops and biosynthetic pathways for cutin polymers, flavor volatiles, oxylipins, and terpenoid compounds. It chronicles the use of lipases and phospholipases in the creation of structured lipids and fats, including cocoa butter, low-calorie fats, and Betapol, and emerging methods using supercritical carbon dioxide as a benign solvent for lipid analysis, fractionation, and enzymatic reaction. It also covers reaction conditions, reactor design, solvent selection, immobilization technology, and enzyme sources for optiml large-scale manufacturing, and describes the formation of oxylipins through the lipoxygenase pathway, as well as other unusual fatty acids. The authors provide in-depth analyses of the structure, metabolic and enzymatic functions and mechanisms, defensive and catalytic properties, industrial uses, and other applications of oxilipins and lipases.

Nutraceutical Fatty Acids from Oleaginous Microalgae

Nutraceutical Fatty Acids from Oleaginous Microalgae
Author: Alok Kumar Patel
Publisher: John Wiley & Sons
Total Pages: 368
Release: 2020-08-11
Genre: Technology & Engineering
ISBN: 1119631718

Over the past several years, extensive research has been done on the microbial production of polyunsaturated fatty acids (PUFA). Regardless, research on the oleaginous microalgae used as feedstock for biofuels production and the overall story about the production of nutraceutical fatty acids from oleaginous microalgae has been very limited. This volume provides an exclusive insight on the production of nutraceutical fatty acids from oleaginous microalgae and their role on human health. Some saturated and monounsaturated fatty acids can be synthesized by humans, whereas long-chain polyunsaturated fatty acids (PUFAs) such as α-linolenic acid and linoleic acid cannot and are deemed essential. The products of these acids, such as DHA, which is important for early visual and neurological development, are extremely important to human health. Replacing SFAs with omega-3 and omega-6 fatty acids in the diet reduce the risk of cardiovascular diseases and prevent Alzheimer's, bipolar disorder, and schizophrenia, among other benefits. The ever-rising global demand for omega-3 & 6 PUFAs, however, cannot be met solely by fish oil, due to diminishing fish stocks and pollution of marine ecosystems, which has led to increased interest in alternative sustainable sources. Vegetable oils from genetically engineered plant oilseeds and microorganisms are two potential alternatives to fish oil, even though omega-3 PUFAs are highest in the latter. Although transgenic plants present numerous advantages, their production is dependent on seasonal and climatic conditions and the availability of arable land. Moreover, there are public concerns regarding the cultivation of transgenic crops in open ecosystems. These, together with regulatory issues restrict the large-scale production of genetically modified crops. Microorganisms, however, are known natural producers of microbial oils similar to those obtained from plants and animals and a possible source of nutritionally important omega-3 & 6 PUFAs. This groundbreaking volume presents invaluable new research on essential fatty acids, their production from various oleaginous microorganisms, biochemical and metabolic engineering to improve PUFAs content in oil, extraction and purification of omega 3 fatty acids, and the current market scenario. Whether a veteran engineer or scientist using it as a reference or a professor using it as a textbook, this outstanding new volume is a must-have for any engineer or scientist working in food science.

Implementation of Enzymatic Processes and Lactic Bacteria in the Food Industries

Implementation of Enzymatic Processes and Lactic Bacteria in the Food Industries
Author: Mohamed Ghoul
Publisher: John Wiley & Sons
Total Pages: 324
Release: 2024-08-15
Genre: Business & Economics
ISBN: 1394312326

Consumers are demanding healthy, natural food products with no environmental impacts. The use of ingredients of plant origin and the implementation of bioprocesses using enzymes and micro-organisms as biocatalysts represent a promising alternative to satisfy this demand. Implementation of Enzymatic Processes and Lactic Bacteria in the Food Industries focuses on describing the latest developments in the use of enzymatic biocatalysts and lactic acid bacteria in the food industry. The first part of the book is devoted to the presentation of different classes of enzymes, production and application processes, ways of improving enzymes and the main industrial applications using biocatalysts. The second part of the book describes a family of micro-organisms widely used in health food processing and formulation: lactic acid bacteria and bifidobacteria. Then, the most commonly used encapsulation matrices, encapsulation processes and the process of bacterial adhesion to these matrices are discussed. Finally, the best-known fermented foods and new approaches developed in this field are presented.

Value-Addition in Food Products and Processing Through Enzyme Technology

Value-Addition in Food Products and Processing Through Enzyme Technology
Author: Mohammed Kuddus
Publisher: Academic Press
Total Pages: 540
Release: 2021-12-01
Genre: Science
ISBN: 0323903762

Value Addition in Food Products and Processing using Enzyme Technology offers an updated review regarding the potential impact of new enzymes and enzyme technology on the food sector. The book brings together novel sources and technologies regarding enzymes in value added food development, food production, food processing, food preservation, food engineering and food biotechnology. It will be extremely useful for different types of readers, including food scientists, academic and food biotechnologists, but will also be ideal for students studying food-related courses. This book includes concise and up-to-date research information from multiple independent scientific papers from around the world. This is a essential, multidisciplinary text for research and development professionals, research scientists, and academics in food, biotechnology, and agriculture industries. It addresses safety issues and includes the sources, screening, immobilization and application of food-grade enzymes in food. Presents research data from experts Includes emerging industry topics such as baby food and food safety Offers methodologies of enzymes in diagnostics for food testing and analysis Emphasizes enzyme technology through a microbial biotechnological lens Includes bakery and confectionery products, meat and poultry products, vegetables, food ingredients, functional foods, flavors and food additives and seafood

Biomass, Biofuels, Biochemicals

Biomass, Biofuels, Biochemicals
Author: Carlos Ricardo Soccol
Publisher: Elsevier
Total Pages: 378
Release: 2022-07-07
Genre: Technology & Engineering
ISBN: 0323998135

Microbial Lipids: Processes, Products, and Innovations brings together experienced authors on microbial lipids bioproduction, introducing the reader to key aspects from lipid chemistry and biochemistry to industrial production by fungi, yeast and microalgae. Intended for professionals in industry-oriented research and development, this volume also describes lipid production using synthetic media and agroindustry residues and discusses the potential of integration of microbial lipids in biorefineries, towards a circular economy. With a market of US$ 7 billion, oils and fats are one of the most important classes of natural products, with applications in food, feed, nutraceuticals, cosmetics, biofuel, and oleo chemistry. Primary sources include plants, animals and fish. However, specialty lipids such as oils rich in omega-3 and -6 fatty acids have limited sources and productivity. Microbial oils are gaining space in the global market as green – and even vegan – alternatives to traditional sources. Microorganisms also have high productivity and metabolic plasticity which makes it promising sources of new products. Includes key developments in lipids chemistry and biochemistry Provides technological developments on the production and applications of lipids from various microbial sources Covers details on non-conventional lipids Presents the latest advances on the downstream processing of lipids