Biochemistry in the Lab

Biochemistry in the Lab
Author: Benjamin F. Lasseter
Publisher: CRC Press
Total Pages: 161
Release: 2019-09-30
Genre: Medical
ISBN: 0429957378

Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience Includes chemical biology as its foundation, teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute, at comparatively low cost Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.

Biochemistry Laboratory Manual For Undergraduates

Biochemistry Laboratory Manual For Undergraduates
Author: Timea Gerczei Fernandez
Publisher: Walter de Gruyter GmbH & Co KG
Total Pages: 190
Release: 2015-03-11
Genre: Science
ISBN: 3110426250

Biochemistry laboratory manual for undergraduates – an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.

Laboratory Manual of Microbiology, Biochemistry and Molecular Biology

Laboratory Manual of Microbiology, Biochemistry and Molecular Biology
Author: J. Saxena
Publisher: Scientific Publishers
Total Pages: 402
Release: 2015-05-01
Genre: Technology & Engineering
ISBN: 9386237237

Though many practical books are available in the market but this Laboratory Manual of Microbiology, Biochemistry and Molecular Biology is an unique combination of protocols that covers maximum (about 80%) of the practicals of various Indian universities for UG and PG courses in Bioscience, Biotechnology, Microbiology, Biochemistry and Biochemical Engineering.

Biochemistry Practical Manual - E-Book

Biochemistry Practical Manual - E-Book
Author: Soundravally Rajendiran
Publisher: Elsevier Health Sciences
Total Pages: 193
Release: 2019-01-28
Genre: Medical
ISBN: 813125352X

This book will serve as a practical manual for undergraduate students in MBBS. Related clinical concepts will also be useful in the preparation of postgraduate entrance exams. This book will serve as a practical manual for undergraduate students in MBBS. Related clinical concepts will also to useful in the preparation of Post-graduate entrance exams.

Manual of Practical Medical Biochemistry

Manual of Practical Medical Biochemistry
Author: Evangeline Jones
Publisher: Jaypee Brothers Medical Publishers Pvt Limited
Total Pages: 0
Release: 2022-03-30
Genre: Medical
ISBN: 9789354655104

1. General General Instructions Laboratory Equipments and Procedures Buffers and pH Quality Control 2. Analysis of Urine and Cerebrospinal Fluid Normal Urine: Characteristics and Analysis Abnormal Constituents of Urine Screening of Urine for Inborn Errors of Metabolism and the Use of Paper Chromatography Composition of Cerebrospinal Fluid 3. Quantitative Experiments Principles of Colorimetry Principles of Spectrophotometer Estimation of Glucose Glucose Tolerance Test Estimation of Serum and Urine Creatinine: Creatinine Clearance Estimation of Urea Serum Proteins--Albumin: Globulin Ratio Serum Total Cholesterol Lipid Profile Serum Calcium and Phosphorus Serum Bilirubin Serum Transaminases Serum Alkaline Phosphatase Serum Uric Acid 4. Equipments And Procedures Point of Care Testing pH Meter Paper Chromatography and Thin Layer Chromatography Protein Electrophoresis Polyacrylamide Gel Electrophoresis Electrolyte Analysis Arterial Blood Gas Analyzer Enzyme-Linked Immunosorbent Assay Immunodiffusion Autoanalyzer DNA Isolation from Blood/Tissues 5. Function Tests Gastric Function Tests and Analysis of Gastric Juice Pancreatic Function Tests Liver Function Tests Analysis of Bile Renal Function Tests Thyroid Function Tests Adrenal Function Tests 6. Food and Energy Energy Content of Food and Glycemic Index Fats in Food 7. Clinical Case Studies Basis and Rationale of Biochemical Tests Done in Certain Clinical Conditions 8. Objective Structured Practical Examination Objective Structured Practical Examination 9. Reagents Reagent Preparation 10. Normal Values Normal Values Worksheet

A Textbook of Practical Biochemistry

A Textbook of Practical Biochemistry
Author: Joshi A. Rashmi
Publisher: B. Jain Publishers
Total Pages: 212
Release: 2002
Genre: Medical
ISBN: 9788180560378

Based on BHMS syllabus by CCH.This book deals with the basic knowledge of practical biochemistry but also its application in actual clinical practice.Creates an abiding interest in the practical aspects of the subject.

Molecular Biology Techniques

Molecular Biology Techniques
Author: Heather B. Miller
Publisher: Academic Press
Total Pages: 227
Release: 2011-10-18
Genre: Science
ISBN: 0123855454

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions