Stable Isotope Techniques in the Study of Biological Processes and Functioning of Ecosystems

Stable Isotope Techniques in the Study of Biological Processes and Functioning of Ecosystems
Author: M.J. Unkovich
Publisher: Springer Science & Business Media
Total Pages: 312
Release: 2001-07-31
Genre: Medical
ISBN: 9780792370789

This book has a very strong practical orientation, telling readers what methodologies are available using stable isotopes, how studies should be designed and executed to maximise effectiveness and incisiveness in terms of data obtained and outcomes in terms of understanding events and processes in plant and ecosystem functioning. The readership is very much aimed toward postgraduates with good knowledge of general biological principles and underlying chemical and physical processes. Pre-existing knowledge of or experience in application and assay of stable isotopes is not required. The stable isotope research field is one of the most rapidly growing areas of ecophysiology and techniques using stable isotopes comprise an ever increasing component of research programs of university postgraduates and a wide range of agencies conducting environmental monitoring and rehabilitation programs. The book is tailor-made for such an audience.

Carbon Isotope Techniques

Carbon Isotope Techniques
Author: David C. Coleman
Publisher: Academic Press
Total Pages: 287
Release: 2012-12-02
Genre: Science
ISBN: 032315767X

Carbon Isotope Techniques deals with the use of carbon isotopes in studies of plant, soil, and aquatic biology. Topics covered include photosynthesis/translocation studies in terrestrial ecosystems; carbon relationships of plant-microbial symbioses; microbe/plant/soil interactions; and environmental and aquatic toxicology. Stable carbon isotope ratios of natural materials are also considered. Comprised of 15 chapters, this book begins with an introduction to radiation-counting instruments used in measuring the radioactivity in soil and plant samples containing carbon-14. The discussion then turns to the basic methods of 14C use in plant science, highlighted by three examples of applications in the field of plant physiology and ecology. Subsequent chapters explore the use of carbon isotope techniques for analyzing the carbon relationships of plant-microbial symbioses; the interactions of microbes, plants, and soils; and the degradation of herbicides and organic xenobiotics. Carbon dating and bomb carbon are also described. The final section is devoted to the uses and procedures for 13C and 11C. This monograph is intended for advanced undergraduate or graduate students, as well as generalist scientists who have not previously used radioisotopes or stable isotopes in their research.

Isotope Techniques in Biological Sciences

Isotope Techniques in Biological Sciences
Author: U.S. Atomic Energy Commission
Publisher:
Total Pages: 48
Release: 1958
Genre: Biology
ISBN:

This bibliography contains 807 selected references on techniques for the use of radioactive and stable isotopes in biological sciences. These references were taken from the 1948-1956 open literature. A list of the journals from which they were selected and an author index are also included.

Stable Isotopes and Biosphere - Atmosphere Interactions

Stable Isotopes and Biosphere - Atmosphere Interactions
Author: Lawrence B Flanagan
Publisher: Elsevier
Total Pages: 334
Release: 2004-12-15
Genre: Science
ISBN: 0080525288

The emerging multidisciplinary field of earth system science sets out to improve our understanding functioning ecosystems, at a global level across the entire planet. Stable Isotopes and Biosphere - Atmosphere Interactions looks to one of its most powerful tools — the application of stable isotope analyses — to understanding biosphere-atmosphere exchange of the greenhouse gases, and synthesizes much of the recent progress in this work. Stable Isotopes and Biosphere - Atmosphere Interactions describes recent progress in understanding the mechanisms, processes and applications of new techniques. It makes a significant contribution to the emerging, multidisciplinary study of the Earth as an interacting system. This book will be an important reference for students and researchers in biology, ecology, biogeochemistry, meteorology, and atmospheric science and will be invaluable for anyone with any interest in the future of the planet. - Describes applications of new stable isotope techniques to the emerging fields of earth system science and global change - Illustrates advances in scaling of physiological processes from leaf/soil to the global scale - Contains state-of-the-art, critical reviews written by international researchers and experts

Stable Isotope Probing

Stable Isotope Probing
Author: Marc G. Dumont
Publisher: Humana
Total Pages: 247
Release: 2020-08-13
Genre: Science
ISBN: 9781493997237

This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.

Isotopes for Medicine and the Life Sciences

Isotopes for Medicine and the Life Sciences
Author: Institute of Medicine
Publisher: National Academies Press
Total Pages: 144
Release: 1995-01-27
Genre: Medical
ISBN: 0309176697

Radioactive isotopes and enriched stable isotopes are used widely in medicine, agriculture, industry, and science, where their application allows us to perform many tasks more accurately, more simply, less expensively, and more quickly than would otherwise be possible. Indeed, in many casesâ€"for example, biological tracersâ€"there is no alternative. In a stellar example of "technology transfer" that began before the term was popular, the Department of Energy (DOE) and its predecessors has supported the development and application of isotopes and their transfer to the private sector. The DOE is now at an important crossroads: Isotope production has suffered as support for DOE's laboratories has declined. In response to a DOE request, this book is an intensive examination of isotope production and availability, including the education and training of those who will be needed to sustain the flow of radioactive and stable materials from their sources to the laboratories and medical care facilities in which they are used. Chapters include an examination of enriched stable isotopes; reactor and accelerator-produced radionuclides; partnerships among industries, national laboratories, and universities; and national isotope policy.

Nitrogen Isotope Techniques

Nitrogen Isotope Techniques
Author: Roger Knowles
Publisher: Academic Press
Total Pages: 336
Release: 1992-10
Genre: Science
ISBN:

This book is the first laboratory manual to bring together basic procedures for measurement of stable and radioactive isotopes of nitrogen, with specific applications to plant, soil, and aquatic biology. This bench-top reference gives practical coverage of mass and emission spectrometry, nitrogen fixation, nitrification, and identification, organic nitrogen, and the radioactive isotope 13N. Methods are described so that researchers can adapt them, without the aid of outside references, to virtually any task they may encounter in investigations of nitrogen transformation processes. Serves as a practical guide for nitrogen isotope techniques Features studies of nitrogen transformations in terrestrial and aquatic systems Includes basic measurement techniques plus specific applications for stable and radioactive nitrogen isotopes Presents detailed protocols, overviews, and key references Includes fifty figures and sixteen tables Hands-on reference for both students and researchers

Stable Isotopes in Ecology and Environmental Science

Stable Isotopes in Ecology and Environmental Science
Author: Robert Michener
Publisher: John Wiley & Sons
Total Pages: 592
Release: 2008-04-15
Genre: Science
ISBN: 0470691174

This book highlights new and emerging uses of stable isotope analysis in a variety of ecological disciplines. While the use of natural abundance isotopes in ecological research is now relatively standard, new techniques and ways of interpreting patterns are developing rapidly. The second edition of this book provides a thorough, up-to-date examination of these methods of research. As part of the Ecological Methods and Concepts series which provides the latest information on experimental techniques in ecology, this book looks at a wide range of techniques that use natural abundance isotopes to: follow whole ecosystem element cycling understand processes of soil organic matter formation follow the movement of water in whole watersheds understand the effects of pollution in both terrestrial and aquatic environments study extreme systems such as hydrothermal vents follow migrating organisms In each case, the book explains the background to the methodology, looks at the underlying principles and assumptions, and outlines the potential limitations and pitfalls. Stable Isotopes in Ecology and Environmental Science is an ideal resource for both ecologists who are new to isotopic analysis, and more experienced isotope ecologists interested in innovative techniques and pioneering new uses.

Stable Isotope Ecology

Stable Isotope Ecology
Author: Brian Fry
Publisher: Springer Science & Business Media
Total Pages: 318
Release: 2007-01-15
Genre: Science
ISBN: 0387337458

A solid introduction to stable isotopes that can also be used as an instructive review for more experienced researchers and professionals. The book approaches the use of isotopes from the perspective of ecological and biological research, but its concepts can be applied within other disciplines. A novel, step-by-step spreadsheet modeling approach is also presented for circulating tracers in any ecological system, including any favorite system an ecologist might dream up while sitting at a computer. The author’s humorous and lighthearted style painlessly imparts the principles of isotope ecology. The online material contains color illustrations, spreadsheet models, technical appendices, and problems and answers.