Isolation, Characterization, and in Vitro Proliferation of Canine Bone Marrow, Adipose Tissue, Muscle, and Periosteum-derived Mesenchymal Stem Cells

Isolation, Characterization, and in Vitro Proliferation of Canine Bone Marrow, Adipose Tissue, Muscle, and Periosteum-derived Mesenchymal Stem Cells
Author: Agatha H. Kisiel
Publisher:
Total Pages:
Release: 2012
Genre:
ISBN:

Procedures: Characterization of MSCs was based on their plastic adherence and morphology, immunofluorescence of MSC-associated cell surface markers, and expression of pluripotency-associated transcription factors. Morphological and histochemical methods were used to evaluate differentiation of MSCs cultured in adipogenic, osteogenic, and chondrogenic media. Passage one MSCs, cultured in triplicate, were counted at 24, 48, 72, and 96 hours to determine tissue specific-MSC proliferative capacity. Mesenchymal stem cell yield/gram of tissue was calculated for confluent passage one MSCs.

Mesenchymal Stem Cell in Veterinary Sciences

Mesenchymal Stem Cell in Veterinary Sciences
Author: Mudasir Bashir Gugjoo
Publisher: Springer Nature
Total Pages: 347
Release: 2020-11-09
Genre: Science
ISBN: 9811560374

This book focuses on mesenchymal stem cells (MSCs) of animal origin, including their isolation, characterization, and clinical applications. After briefly discussing the historical development of the field of stem cell research, it describes the basic properties and nature of stem cells particularly in relation to MSCs. In turn, it reviews materials and methods used to isolate MSCs from various sources, culture expansion, characterization and long-term storage. It also explores the therapeutic efficacy, immunomodulation and anti-inflammatory, and differentiation properties of MSCs. Importantly, the book discusses the applications of genetic engineering to enhance the efficacy and potential of MSCs in regenerative medicine. The book largely addresses the potential applications of mesenchymal stem cells in therapies for important species of domesticated animals including sheep, goats, cattle, buffalo, cats, dogs and horses. Finally, the book presents an abridgement of challenges and future prospects of stem cell research and application in medicine, in general and veterinary sciences, in particular.

Placenta

Placenta
Author: Ornella Parolini
Publisher: CRC Press
Total Pages: 281
Release: 2016-02-22
Genre: Medical
ISBN: 1498788173

Long regarded as biological waste, the placenta is gaining momentum as a viable product for clinical use. Due to their unique properties, placental cells and derivatives show great promise in curing various diseases. Utilizing contributions from world-renowned experts, Placenta: The Tree of Life considers the therapeutic potential of these cells. I

Urology, An Issue of Veterinary Clinics of North America: Small Animal Practice

Urology, An Issue of Veterinary Clinics of North America: Small Animal Practice
Author: Mary Labato
Publisher: Elsevier Health Sciences
Total Pages:
Release: 2019-03-28
Genre: Medical
ISBN: 0323678491

This issue of Veterinary Clinics: Small Animal Practice, edited by Dr. Mary Anna Labato and Dr. Mark J. Acierno, focuses on current topics in Urology. Articles include: Urinary incontinence; Inappropriate urinations; Upper tract urolithiasis; Nutritional management of urolithiasis; Feline Idiopathic Cystitis (FIC); Urinary Tract Infection (UTI); Stem cell therapy; Importance of urinalysis; Urinary tract cytology; DI of the urinary tract; Interventional therapies of the urinary tract; Urologic oncology; and Nutraceuticals for urine tract use.

Recent Advances in Stem Cells

Recent Advances in Stem Cells
Author: Essam M. Abdelalim
Publisher: Springer
Total Pages: 276
Release: 2016-09-14
Genre: Medical
ISBN: 3319332708

This volume explores recent advances in the use of pluripotent stem cells (PSCs) and adult stem cells (ASCs) in basic and clinical applications. The chapters discuss use of PSCs for drug screening, genome editing, modeling of kidney, motor neuron diseases, and diabetes as well as their application in cancer; ASCs are discussed in the contexts of banking of umbilical cord stem cells, use of multipotential stromal cells (MSCs) for bone repair, cellular interactions during fracture repair stages, and therapeutic applications of neural crest stem cells and lung stem cells. The text is organized by sections dealing with PSCs and ASCs specifically, presenting the reader with a comprehensive examination of both these forms of stem cells. Expertly authored and drawing from a wealth of international perspectives, Recent Advances in Stem Cells: From Basic Research to Clinical Applications presents a succinct yet detailed review of cutting-edge research in this rapidly expanding field. This installment of Springer’s Stem Cell Biology and Regenerative Medicine series is essential reading for academics, researchers, and clinicians in the fields of cell biology, genetics, nephrology, osteology, oncology, and pulmonology.

August's Consultations in Feline Internal Medicine, Volume 7 - E-Book

August's Consultations in Feline Internal Medicine, Volume 7 - E-Book
Author: Susan Little
Publisher: Elsevier Health Sciences
Total Pages: 1085
Release: 2015-11-25
Genre: Medical
ISBN: 0323243398

Stay current with cutting-edge information from the leading feline experts! August’s Consultations in Feline Internal Medicine, Volume 7 takes the popular Current Therapy approach to the latest issues, advances, and therapies in feline care. The 103 new chapters are organized by body systems, making information easy to access, and include more than 800 new detailed photographs, diagrams, and MRI and ultrasound images. Discussions of scientific findings always emphasize clinical relevance and practical application. This edition addresses new topics ranging from feline obesity and food allergies to respiratory mycoplasmal infections. From feline expert Susan Little, with chapters written by more than 130 international specialists, this practical resource will be an invaluable addition to every small animal clinician's library. The Current Therapy format focuses on the latest advances in feline care and includes broad, traditional, and controversial subjects of real clinical importance. Clinically relevant approach is supported with scientific research and promotes practical, progressive clinical management. More than 130 world-renowned contributors provide expert insight across the full spectrum of feline internal medicine. Extensive references make it easy to find additional information about specific topics most important to your practice. 103 ALL-NEW chapters reflect the latest findings, reports, and evidence-based coverage of pressing topics such as: Managing respiratory mycoplasmal infections Continuous glucose monitoring in cats with diabetes Feline food allergy Stem cell therapy for chronic kidney disease Feline idiopathic cystitis Electrochemotherapy Current concepts in preventing and managing obesity Recognition and treatment of hypertensive crises Feline social behavior and personality NEW Emergency and Critical Care Medicine section contains 13 chapters covering complicated and serious internal medical problems. NEW! More than 800 colorful new images clarify concepts and demonstrate clinical examples.

Veterinary Surgery: Small Animal Expert Consult - E-BOOK

Veterinary Surgery: Small Animal Expert Consult - E-BOOK
Author: Spencer A. Johnston
Publisher: Elsevier Health Sciences
Total Pages: 2600
Release: 2017-06-14
Genre: Medical
ISBN: 032332049X

Focus on the "how" and "why" of medical/surgical conditions — the critical issues that lead to successful outcomes for your patients — with Veterinary Surgery: Small Animal, Second Edition. This two-volume full-color resource offers an authoritative, comprehensive review of disease processes, a thorough evaluation of basic clinical science information, and in-depth discussion of advanced surgeries. With an updated Expert Consult website you can access anytime and detailed coverage of surgical procedures, it is the definitive reference for surgical specialists, practicing veterinarians, and residents. Expert Consult website offers access to the entire text online, plus references linked to original abstracts on PubMed. Comprehensive coverage includes surgical biology, surgical methods and perioperative care, neurosurgery, and orthopedics in Volume One, and all soft tissue surgery organized by body system in Volume Two. Extensive references to published studies available on Expert Consult show the factual basis for the material. Strong blend of clinical and basic science information facilitates a clear understanding of clinical issues surrounding operative situations. Highly recognized contributing authors create chapters from their own experience and knowledge base, providing the most authoritative, current information available. Coverage of anatomy, physiology, and pathophysiology in chapters on specific organs includes information critical to operative procedures and patient management. In-depth chapters on anesthesia, surgical oncology, tumors of the spine, and musculoskeletal neoplasia provide valuable resources for practicing surgeons, especially in the area of cancer treatment. Preoperative considerations and surgical implications for surgical procedures help surgeons make decisions about treatment approaches. NEW and UPDATED! Expert Consult website with print text plus complete online access to the book’s contents, so you can use it anytime — anywhere. EXPANDED! Coverage of interventional radiology techniques in Volume Two (soft tissue volume) to provide cutting-edge information on contemporary imaging modalities that gain access to different structures of the patient’s body for diagnostic and therapeutic reasons. NEW and UPDATED! Expanded coverage of coaptation devices and small animal prosthetics clearly explains how they are used in a variety of clinical situations. EXPANDED! Principles of minimally invasive plate treatment added to Volume One (orthopedic volume) to show how these advancements maximize healing and protect the patient while meeting the surgeon’s goals in using fracture fixation.

Stem Cells in Regenerative Medicine

Stem Cells in Regenerative Medicine
Author: Alain A. Vertes
Publisher: John Wiley & Sons
Total Pages: 788
Release: 2015-09-14
Genre: Science
ISBN: 1118846214

This book is a unique guide to emerging stem cell technologies and the opportunities for their commercialisation. It provides in-depth analyses of the science, business, legal, and financing fundamentals of stem cell technologies, offering a holistic assessment of this emerging and dynamic segment of the field of regenerative medicine. • Reviews the very latest advances in the technology and business of stem cells used for therapy, research, and diagnostics • Identifies key challenges to the commercialisation of stem cell technology and avenues to overcome problems in the pipeline • Written by an expert team with extensive experience in the business, basic and applied science of stem cell research This comprehensive volume is essential reading for researchers in cell biology, biotechnology, regenerative medicine, and tissue engineering, including scientists and professionals, looking to enter commercial biotechnology fields.