Isoelectric Focusing Theory Methodology And Application
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Author | : Pier Giorgio Righetti |
Publisher | : Elsevier |
Total Pages | : 385 |
Release | : 2000-04-01 |
Genre | : Science |
ISBN | : 0080858805 |
This book deals with theoretical and practical developments of IEF and offers detailed methodology for many of the commonly used procedures, such as IEF in gels. It is intended both as a reference guide and a practical manual.
Author | : Nicholas Catsimpoolas |
Publisher | : Elsevier |
Total Pages | : 278 |
Release | : 2012-12-02 |
Genre | : Science |
ISBN | : 0323158056 |
Isoelectric Focusing deals with the theory and applications of isoelectric focusing. The book reviews some alternative approaches to generation of pH gradients for isoelectric focusing analysis, along with other relevant topics such as the theoretical aspects of steady-state isoelectric focusing, gel electrofocusing/gel electrophoresis, and transient state isoelectric focusing. This volume is organized into nine chapters and begins by looking at the history of the discovery of isoelectric focusing and correcting some blurred impressions conveyed in the literature. It then turns to a discussion of the differential equation of isoelectric focusing, the carrier ampholytes used in carrying out the technique, the use of isoelectric focusing in polyacrylamide and granulated gels as well as in free solution, and continuous-flow electrophoresis and isoelectric focusing. An attempt is made to illuminate problems in isoelectric focusing and to point out possible solutions in the areas of theory, mechanisms, ampholyte chemistry, and apparatus. Some of the processes that occur during an isoelectric focusing experiment are also considered. This book is intended for anyone who needs to know what isoelectric focusing is all about.
Author | : T. S. Work |
Publisher | : |
Total Pages | : 402 |
Release | : 1983 |
Genre | : Isoelectric focusing |
ISBN | : 9780444804983 |
Hardbound. This book deals with theoretical and practical developments of IEF and offers detailed methodology for many of the commonly used procedures, such as IEF in gels. It is intended both as a reference guide and a practical manual.
Author | : P. G. Righetti |
Publisher | : |
Total Pages | : 386 |
Release | : 1983 |
Genre | : Isoelectric focusing |
ISBN | : 9780720442007 |
Author | : Donald H. Whitmore |
Publisher | : CRC Press |
Total Pages | : 366 |
Release | : 1990-09-24 |
Genre | : Science |
ISBN | : 9780849364167 |
Probably the most ubiquitous biochemical method used today for examining the genetics of individuals, populations, or phylogenetic relationships between taxa is electrophoresis. This book has been created to offer a viewpoint regarding current electrophoretic separation methodologies of macromolecules and their major applications to fisheries management. The chapters in this book have been selected and organized into three sections to create a carefully blended mixture of methodologies and applications designed to educate the novice, as well as stimulate interest in professional researchers currently using electrophoresis for their work. The first section includes chapters that discuss the principles that explain the genetic basis of multiple molecular forms of proteins, the theory and practice of DNA analyses, and the methodology of electrophoretic separation of these macromolecules; starch gel electrophoresis as the predominant electrophoretic tool for fisheries genetics; and protein isoelectric focusing and DNA analysis. The second section describes a variety of applications for electrophoretic techniques. The third section presents a discussion and results of experiments conducted by Dennis Powers and his associates regarding the physiological significance of multiple forms of enzymes using the fish Fundulus heteroclitus as a model system. The book features a catalog of nearly 100 enzyme staining recipes and covers new areas in electrophoretic work, such as DNA fingerprinting, genetic tags, mitochondrial DNA methodologies, and genomic manipulation of fish stocks. This book will provide a useful reference resource for fisheries biologists at federal, state, and local levels; fisheries researchers at universities; and students pursuing degrees involving research in fish genetics.
Author | : |
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Total Pages | : 163 |
Release | : 1982 |
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Author | : Pharmacia Fine Chemicals |
Publisher | : |
Total Pages | : 163 |
Release | : 1982 |
Genre | : Isoelectric focusing |
ISBN | : |
Author | : B. Andrew Jackiw |
Publisher | : |
Total Pages | : 356 |
Release | : 1983 |
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Author | : Harry Roman Drab |
Publisher | : |
Total Pages | : 272 |
Release | : 1971 |
Genre | : |
ISBN | : |
Author | : Pier Giorgio Righetti |
Publisher | : |
Total Pages | : |
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