Introduction To Subcellular Biochemistry
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| Author | : Paul R. Pryor |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2015 |
| Genre | : Cell fractionation |
| ISBN | : 9781621820420 |
Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.
| Author | : Bruce Alberts |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2002 |
| Genre | : Cytology |
| ISBN | : 9780815332183 |
| Author | : Sam Aronoff |
| Publisher | : |
| Total Pages | : 348 |
| Release | : 1961 |
| Genre | : Biochemistry |
| ISBN | : |
| Author | : Donald Roodyn |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 399 |
| Release | : 2013-11-11 |
| Genre | : Science |
| ISBN | : 1461579422 |
The transition from the quarterly Sub-Cellular Biochemistry to the annual SUBCELLULAR BIOCHEMISTRY is a good opportunity to restate the aims and scope of this publication. They were originally given (in Volume 1 No. 1) as follows: This review and essay journal . . . brings together work on a wide range of topics in sub-cellular biochemistry in the hope of stimulating progress towards an integrated view of the cell. It deals with the biochemistry and general biology of nuclei, mitochondria, lysosomes, peroxisomes, chloroplasts, cell membranes, ribosomes, cell sap, flagellae and other specialized cell components. In addition to articles dealing with conventional biochemical studies on sub-cellular struc tures, the journal publishes articles on the genetics, evolution and biogenesis of cell organelles, bioenergetics, membrane behaviour and the interaction between cell structures, particularly between nucleus and cytoplasm. The first four volumes (in the quarterly format) fulfilled many, but not all, of these stated aims, and it is hoped that further articles in the new annual series will soon fill any deficiencies in the range of topics covered. Over the years we have intentionally not interpreted the title of the publication in a too literal sense. Although we have included specific articles on individual subcellular fractions (and certainly hope to do so again) the publication is definitely not only concerned with studies on the biochemistry of isolated cell fractions. The primary target is the "integrated view of the cell.
| Author | : Dennis E. Buetow |
| Publisher | : Elsevier |
| Total Pages | : 545 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323146546 |
The Biology of Euglena, Volume IV: Subcellular Biochemistry and Molecular Biology focuses on the subcellular biochemistry and molecular biology of eukaryotic microorganisms that belong to the genus Euglena, including Euglena gracilis. It investigates enzymes and their functional location in Euglena cells, along with subcellular particles, the nucleus, the mitochondria, the chloroplast protein synthesis and chloroplast DNA, and the microbodies and lysosomes of Euglena. Organized into eight chapters, this volume begins with an overview of techniques in determining the location of enzymes and in isolating organelles in Euglena. It then proceeds with a discussion of the nucleus, its ultrastructure and macromolecules, and chromatin organization. The next chapters examine the morphology and ultrastructure of mitochondria, the morphology and biogenesis of microbodies and lysosomes, the nuclear-cytoplasmic interaction, and the structure and physicochemical properties of chloroplast DNA. The last two chapters consider the ribosomal RNAs of Euglena and the organization and activities of cytoplasmic, mitochondrial, and chloroplast ribosomes and polyribosomes, along with its polyadenylated and messenger RNA. This book will be of interest to biochemists, molecular biologists, botanists, and plant geneticists.
| Author | : J. Robin Harris |
| Publisher | : Springer |
| Total Pages | : 526 |
| Release | : 2019-02-18 |
| Genre | : Medical |
| ISBN | : 9811328358 |
This new volume in the Subcellular Biochemistry series will focus on the biochemistry and cellular biology of aging processes in human cells. The chapters will be written by experts in their respective fields and will focus on a number of the current key areas of research in subcellular aging research. Main topics for discussion are mitochondrial aging, protein homeostasis and aging and the genetic processes that are involved in aging. There will also be chapters that are dedicated to the study of the roles of a variety of vitamins and minerals on aging and a number of other external factors (microbiological, ROS, inflammation, nutrition). This book will provide the reader with a state of the art overview of the subcellular aging field. This book will be published in cooperation with a second volume that will discuss the translation of the cell biology of aging to a more clinical setting and it is hoped that the combination of these two volumes will bring a deeper understanding of the links between the cell and the body during aging.
| Author | : Donald Roodyn |
| Publisher | : Springer |
| Total Pages | : 390 |
| Release | : 1978-05-01 |
| Genre | : Science |
| ISBN | : 9780306334054 |
The transition from the quarterly Sub-Cellular Biochemistry to the annual SUBCELLULAR BIOCHEMISTRY is a good opportunity to restate the aims and scope of this publication. They were originally given (in Volume 1 No. 1) as follows: This review and essay journal . . . brings together work on a wide range of topics in sub-cellular biochemistry in the hope of stimulating progress towards an integrated view of the cell. It deals with the biochemistry and general biology of nuclei, mitochondria, lysosomes, peroxisomes, chloroplasts, cell membranes, ribosomes, cell sap, flagellae and other specialized cell components. In addition to articles dealing with conventional biochemical studies on sub-cellular struc tures, the journal publishes articles on the genetics, evolution and biogenesis of cell organelles, bioenergetics, membrane behaviour and the interaction between cell structures, particularly between nucleus and cytoplasm. The first four volumes (in the quarterly format) fulfilled many, but not all, of these stated aims, and it is hoped that further articles in the new annual series will soon fill any deficiencies in the range of topics covered. Over the years we have intentionally not interpreted the title of the publication in a too literal sense. Although we have included specific articles on individual subcellular fractions (and certainly hope to do so again) the publication is definitely not only concerned with studies on the biochemistry of isolated cell fractions. The primary target is the "integrated view of the cell.
| Author | : Donald B. Roodyn |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 425 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 1461579481 |
The broad aim of SUBCELLULAR BIOCHEMISTRY is to present an inte grated view of the cell in which artificial barriers between disciplines are bro ken down. The contents of Volume 7 illustrate the interconnections between initially unrelated fields of study and show strikingly how advances along one front become possible because of parallel successes in another. Current research into cell organelles and membrane systems is not only concerned with the elucidation of their structure and function. It also asks such questions as: Which regions of the cell are concerned in the bioassembly of the organelle? How are organelle and membrane precursors transported from the site of syn thesis to the newly formed cell constituent? What genetic systems control the biosynthesis and assembly of cell components and how do these systems inter act? How did the various cell constituents evolve? How did the genetic and biosynthetic systems making the organelles themselves evolve? The search for the answer to such questions has placed organelle biochemistry on a different level than that of the more restricted studies of the 1950s and early 1960s and promises to produce some fascinating and surprising results. Volume 7 opens with a detailed chapter by A. A. Hadjiolov on the bio genesis of ribosomes of eukaryotes. The general arrangement of ribosomal genes is discussed, and there is a full account of their transcription.
| Author | : Donald B. Roodyn |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 562 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 1461327091 |
| Author | : Donald B. Roodyn |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 419 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 1461579511 |
In this volume of SUBCELLULAR BIOCHEMISTRY we cover a wide range of topics of considerable biological importance and have continued in our policy of letting authors, rather than editors, decide the "natural" length of their articles. Thus, we have some short but nevertheless significant contributions, as well as more massive chapters. We start with a detailed account by 1. Oelze of the composition and development of the bacterial photosynthetic apparatus. A number of photosynthetic bacteria are discussed, with particular emphasis on the well-studied Rhodospirillum rubrum and Rhodopseudomonas sphae roides. The reader will no doubt be struck by the great wealth of information now available on the molecular organization of the photosynthetic and respi ratory systems in these organisms. Equally important is our improved under standing of the biosynthesis and assembly of these systems. It is now generally accepted that photosynthetic bacteria are excellent model systems for the study of bioenergetic processes. It may well be that they will become equally popular as models for the study of membrane biogenesis, and it is to be hoped that Oelze's erudite and comprehensive treatment of the subject will help in this regard.
