In Vivo Microscopy
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| Author | : R.F. Guthoff |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 207 |
| Release | : 2007-05-16 |
| Genre | : Medical |
| ISBN | : 354032707X |
This lavishly illustrated atlas demonstrates normal in-vivo anatomy of the cornea, limbus, and conjunctiva; quantifies various cellular structures using cell-density calculations; and establishes correlations between novel optical sections of various diseases of the ocular surface and clinical findings. It also describes early signs of different eye diseases and supports the reader in diagnostic and therapeutic management.
| Author | : Josef F. Bille |
| Publisher | : Springer |
| Total Pages | : 411 |
| Release | : 2019-08-13 |
| Genre | : Medical |
| ISBN | : 3030166384 |
This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
| Author | : Ron D. Frostig |
| Publisher | : CRC Press |
| Total Pages | : 0 |
| Release | : 2009-05-06 |
| Genre | : Science |
| ISBN | : 9781420076844 |
These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques and their applications for the growing field of functional imaging in the live brain using optical imaging techniques. New in the Second Edition: Voltage-sensitive dyes imaging in awake behaving animals Imaging based on genetically encoded probes Imaging of mitochondrial auto-fluorescence as a tool for cortical mapping Using pH-sensitive dyes for functional mapping Modulated imaging Calcium imaging of neuronal activity using 2-photon microscopy Fourier approach to optical imaging Fully updated chapters from the first edition Leading Authorities Explore the Latest Techniques Updated to reflect continuous development in this emerging research area, this new edition, as with the original, reaches across disciplines to review a variety of non-invasive optical techniques used to study activity in the living brain. Leading authorities from such diverse areas as biophysics, neuroscience, and cognitive science present a host of perspectives that range from a single neuron to large assemblies of millions of neurons, captured at various temporal and spatial resolutions. Introducing techniques that were not available just a few years ago, the authors describe the theory, setup, analytical methods, and examples that highlight the advantages of each particular method.
| Author | : Margarida Barroso |
| Publisher | : CRC Press |
| Total Pages | : 464 |
| Release | : 2020-12-03 |
| Genre | : Science |
| ISBN | : 1351704494 |
Imaging from Cells to Animals In Vivo offers an overview of optical imaging techniques developed over the past two decades to investigate biological processes in live cells and tissues. It comprehensively covers the main imaging approaches used as well as the application of those techniques to biological investigations in preclinical models. Among the areas covered are cell metabolism, receptor-ligand interactions, membrane trafficking, cell signaling, cell migration, cell adhesion, cytoskeleton and other processes using various molecular optical imaging techniques in living organisms, such as mice and zebrafish. Features Brings together biology and advanced optical imaging techniques to provide an overview of progress and modern methods from microscopy to whole body imaging. Fills the need for a comprehensive view of application-driven development and use of new tools to ask new biological questions in the context of a living system. Includes basic chapters on key methods and instrumentation, from fluorescence microscopy and imaging to endoscopy, optical coherence tomography and super-resolution imaging. Discusses approaches at different length scales and biomedical applications to the study of single cell, whole organ, and whole organism behavior. Addresses the impact on discovery, such as cellular function as implicated in human disease and translational medicine, for example in cancer diagnosis.
| Author | : Andrew M Quinn |
| Publisher | : World Scientific |
| Total Pages | : 341 |
| Release | : 2024-03-06 |
| Genre | : Medical |
| ISBN | : 9813206993 |
In the vast landscape of medical literature, we now have a groundbreaking book that unveils the intricate details of our body's inner workings. In Vivo Microscopy takes readers on a captivating journey through the microscopic realms of organs and systems, offering unprecedented insights into the new technologies that allow the visualization of tissues at a cellular level.Authored by leading experts in the field, this comprehensive guide delves into the cellular mechanisms of various organs, shedding light on the microscopic events underpinning the full spectrum of disease processes, especially inflammatory conditions and cancer. In vivo microscopy allows the visualization of living tissues in real-time. This allows us to explore the dynamic interaction of cells and their microenvironment, providing a profound understanding of the manifestations of each of these conditions.In Vivo Microscopy not only educates but also inspires. Its rich visuals, coupled with compelling narratives, make it an enthralling read for anyone with an interest in the microanatomy of the human body and the novel technologies the book details. It also serves as a valuable resource for healthcare professionals seeking to deepen their understanding of the pathologic basis of disease, as well as for researchers aiming to uncover novel therapeutic targets.As we continue to unravel the complexities of our body, this book serves as a guiding light, encouraging us to investigate the microscopic universe within and paving the way for advancements in the diagnosis, treatment, and prevention of disease.
| Author | : Rainer Hofmann-Wellenhof |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 484 |
| Release | : 2012-02-07 |
| Genre | : Medical |
| ISBN | : 3642219977 |
This book focuses on the use and significance of in vivo reflectance confocal microscopy (RCM) for non-invasive high-resolution imaging of the skin. All of the chapters in this hands-on guide are generously illustrated with numerous confocal images and structured in a reader-friendly way. The contents include detailed information on the most relevant and up-to-date aspects of RCM, schematic drawings summarizing and explaining the most important RCM criteria, and a chapter specifically devoted to bridging the gap between dermoscopy, RCM, and histopathology. At the end of each chapter, core messages recapitulate the most pertinent aspects. Reflectance Confocal Microscopy for Skin Diseases will be a valuable resource for all physicians involved in the diagnosis and treatment of neoplastic and inflammatory skin diseases.
| Author | : Karsten König |
| Publisher | : Walter de Gruyter GmbH & Co KG |
| Total Pages | : 785 |
| Release | : 2018-01-22 |
| Genre | : Science |
| ISBN | : 311043007X |
This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography
| Author | : Jens Rietdorf |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 344 |
| Release | : 2005-06-23 |
| Genre | : Science |
| ISBN | : 9783540236986 |
With contributions by numerous experts
| Author | : Salvador Gonzalez |
| Publisher | : CRC Press |
| Total Pages | : 849 |
| Release | : 2017-07-06 |
| Genre | : Medical |
| ISBN | : 1498757626 |
Reflectance confocal microscopy enables lesions in skin to be examined without excision, but with improved diagnostic accuracy, assessment of dermoscopic-histologic correlation, assessment of surgical margins, as well as speed and convenience for the physician and patient. This extensively enlarged and updated text reviews the current and future state of the art for those involved with the diagnosis and treatment of skin tumors, with a greatly increased amount of material on the expected normal patterns of skin throughout life and on non-melanocytic tumors.
| Author | : Mathilde Kaspi |
| Publisher | : Springer Nature |
| Total Pages | : 127 |
| Release | : 2020-03-28 |
| Genre | : Medical |
| ISBN | : 3030366065 |
This Atlas gives the complete expert opinion on the diagnostic features of eyelid and conjunctival tumors (benign and malignant): a state-of-the-art guide with numerous images, useful for both dermatologists and ophthalmologists. This invaluable resource, illustrating clinical, histological and re fectance confocal microscopy features, first addresses the normal conditions of the ocular surface, then reviews lesions due to epidermal, melanocytic and adnexal tumors. A final part is devoted to conjunctiva conditions, from normal to malignant conjunctival tumors. The high number of illustrations and their description of many ocular surface lesions with in vivo confocal microscopy make this atlas an essential guide for the practitioners of both specialities.
