Immobilisation of DNA on Chips: DNA adsorption on carbonaceous materials

Immobilisation of DNA on Chips: DNA adsorption on carbonaceous materials
Author: S. Alegret
Publisher: Springer Science & Business Media
Total Pages: 224
Release: 2005-11-03
Genre: Science
ISBN: 9783540284376

M.I. Pividori, S. Alegret: DNA Adsorption on Carbonaceous Materials .- F. Luderer, U. Walschus: Immobilization of Oligonucleotides for Biochemical Sensing by Self-Assembled Monolayers: Thiol-Organic Bonding on Gold and Silanization on Silica Surfaces.- Y. Okahata, T. Kawasaki: Preparation and Electron Conductivity of DNA-Aligned Cast and LB Films from DNA-Lipid Complexes.- A. del Campo, I.J. Bruce: Substrate Patterning and Active Strategies for DNA Chip Fabrication.- D.V. Nicolau, P.D. Sawant: Scanning Probe Microscopy Studies of Surface-Immobilised DNA/Oligonucleotide Molecules.- A. Guiseppi-Elie, L. Lingerfelt: Impedimetric Detection of DNA Hybridization: Towards Near Patient DNA Diagnostics

Encyclopedia of Microfluidics and Nanofluidics

Encyclopedia of Microfluidics and Nanofluidics
Author: Dongqing Li
Publisher: Springer Science & Business Media
Total Pages: 2242
Release: 2008-08-06
Genre: Technology & Engineering
ISBN: 0387324682

Covering all aspects of transport phenomena on the nano- and micro-scale, this encyclopedia features over 750 entries in three alphabetically-arranged volumes including the most up-to-date research, insights, and applied techniques across all areas. Coverage includes electrical double-layers, optofluidics, DNC lab-on-a-chip, nanosensors, and more.

A Beginner’s Guide to Microarrays

A Beginner’s Guide to Microarrays
Author: Eric M. Blalock
Publisher: Springer Science & Business Media
Total Pages: 356
Release: 2011-06-27
Genre: Science
ISBN: 1441987606

A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including: -Cleaning and coating glass slides, -Designing oligonucleotide probes, -Constructing arrays for the detection and quantification of different bacterial species, -Preparing spotting solutions, -Troubleshooting spotting problems, -Setting up and running a core facility, -Normalizing background signal and controlling for systematic variance, -Designing experiments for maximum effect, -Analyzing data with statistical procedures, -Clustering data with machine-learning protocols.

Molecular Methods for Virus Detection

Molecular Methods for Virus Detection
Author: Danny L. Wiedbrauk
Publisher: Elsevier
Total Pages: 405
Release: 1995-02-08
Genre: Science
ISBN: 0080536891

Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. - Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions - Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT - Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence - Includes quality control information crucial in research and clinical laboratories - Most chapters are written by the inventors and principal developers of the methodologies - Includes color plates, 77 figures, and 18 tables

Electrochemical DNA Biosensors

Electrochemical DNA Biosensors
Author: Mehmet Sengun Ozsoz
Publisher: CRC Press
Total Pages: 568
Release: 2012-04-23
Genre: Science
ISBN: 9814241776

This book focuses on the basic electrochemical applications of DNA in various areas, from basic principles to the most recent discoveries. The book comprises theoretical and experimental analysis of various properties of nucleic acids, research methods, and some promising applications. The topics discussed in the book include electrochemical detection of DNA hybridization based on latex/gold nanoparticle and nanotubes; nanomaterial-based electrochemical DNA detection; electrochemical detection of microorganism-based DNA biosensors; gold nanoparticle-based electrochemical DNA biosensors; electrochemical detection of the aptamer-target interaction; nanoparticle-induced catalysis for DNA biosensing; basic terms regarding electrochemical DNA (nucleic acids) biosensors; screen-printed electrodes for electrochemical DNA detection; application of field-effect transistors to label free electrical DNA biosensor arrays; and electrochemical detection of nucleic acids using branched DNA amplifiers.

Electrochemical Biosensors

Electrochemical Biosensors
Author: Serge Cosnier
Publisher: CRC Press
Total Pages: 405
Release: 2015-01-26
Genre: Science
ISBN: 9814411477

Since four decades, rapid detection and monitoring in clinical and food diagnostics and in environmental and biodefense have paved the way for the elaboration of electrochemical biosensors. Thanks to their adaptability, ease of use in relatively complex samples, and their portability, electrochemical biosensors now are one of the mainstays of analy

Chemical Sensors and Biosensors

Chemical Sensors and Biosensors
Author: Brian R. Eggins
Publisher: John Wiley & Sons
Total Pages: 298
Release: 2008-04-30
Genre: Science
ISBN: 0470511311

Covering the huge developments in sensor technology and electronic sensing devices that have occurred in the last 10 years, this book uses an open learning format to encourage reader understanding of the subject. An invaluable distance learning book Applications orientated providing invaluable aid for anyone wishing to use chemical and biosensors Key features and subjects covered include the following: Sensors based on both electrochemical and photometric transducers Mass-sensitive sensors Thermal-sensitive sensors Performance factors for sensors Examples of applications Detailed case studies of five selected sensors 30 discussion questions with worked examples and 80 self-assessment questions 140 explanatory diagrams An extensive bibliography

Molecular Beacons

Molecular Beacons
Author: Chaoyong James Yang
Publisher: Springer Science & Business Media
Total Pages: 201
Release: 2013-08-15
Genre: Science
ISBN: 3642391095

Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications. This book is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice. Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA.

Protein Microarray Technology

Protein Microarray Technology
Author: Dev Kambhampati
Publisher: Wiley-Blackwell
Total Pages: 284
Release: 2004-03
Genre: Medical
ISBN:

Protein microarrays soon will become one of the major tools for realizing the goals of drug discovery research in the post-genome era. The book is aimed at life science researchers and scientists working in drug and pharma industry.