Human Molecular Biology Laboratory Manual
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Author | : Stefan Surzycki |
Publisher | : John Wiley & Sons |
Total Pages | : 246 |
Release | : 2008-04-15 |
Genre | : Science |
ISBN | : 0470999241 |
Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use
Author | : Heather B. Miller |
Publisher | : Academic Press |
Total Pages | : 227 |
Release | : 2011-10-18 |
Genre | : Science |
ISBN | : 0123855454 |
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author | : Sue Carson |
Publisher | : Academic Press |
Total Pages | : 294 |
Release | : 2019-03-05 |
Genre | : Science |
ISBN | : 0128157755 |
Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author | : S. K. Gakhar |
Publisher | : |
Total Pages | : 0 |
Release | : 2013-09-30 |
Genre | : |
ISBN | : 9789382332305 |
The objective of this text is to train young teachers from colleges and research institutions so that they can advance their research in various fields of biology. It will also help students at BSc and MSc level to learn the techniques involved in molecular biology. The book contains four chapters providing step-by-step protocols. In addition, it has general instructions for safety procedures.
Author | : David Thompson |
Publisher | : Createspace Independent Publishing Platform |
Total Pages | : 170 |
Release | : 2011-06-14 |
Genre | : |
ISBN | : 9781463530082 |
A laboratory manual for an undergraduate-level cell and molecular biology course.
Author | : Bert Atsma |
Publisher | : Benjamin Cummings |
Total Pages | : 324 |
Release | : 2007-02 |
Genre | : Science |
ISBN | : 9780321490117 |
Designed for the one-semester human biology course, this full-color manual offers activities for 23 laboratory sessions in a variety of formats to allow the instructor to customize these exercises to the needs of their course. The lab manual's depth of coverage invites students to explore fundamental concepts of human biology in a laboratory setting.
Author | : K. V. CHAITANYA |
Publisher | : PHI Learning Pvt. Ltd. |
Total Pages | : 156 |
Release | : 2013-06-21 |
Genre | : Science |
ISBN | : 8120348001 |
This laboratory guide, intended for undergraduate and postgraduate students, includes techniques and their protocols ranging from microscopy to in vitro protein synthesis. Experiments relating to chromosomes study and identifying the phases of cell division are explained. The book lucidly deals with the extraction and characteri-zation of chromatin and techniques for studying its modifications, the gene methodology for identification of mutation and the methodology for isolation of nucleic acids from all types of organisms, such as viruses, fungi, plants and animals. All the protocols have been explained following step-by-step method. Different types of electrophoresis and their techniques, including blotting techniques and the methodology for stripping of probes from membranes for reusing the blot, have also been dealt with. Protocols on modern molecular biology techniques—PCR, restriction enzyme digest, DNA isolation, cloning and DNA sequencing—add weightage to the book. It also gives necessary knowledge of different types of stains, staining techniques, buffers, reagents and media used in the protocols. To help students prepare for answering viva voce questions, the book includes MCQs based on the discussed techniques.
Author | : Gary S. Stein |
Publisher | : John Wiley & Sons |
Total Pages | : 439 |
Release | : 2011-03-04 |
Genre | : Science |
ISBN | : 0470889896 |
Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology Perspectives written by the world leaders in the field Introductory chapters will provide background information The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.
Author | : Gail Begley |
Publisher | : |
Total Pages | : |
Release | : 2013 |
Genre | : |
ISBN | : 9781600366772 |
Author | : Charles J. Welsh |
Publisher | : Jones & Bartlett Learning |
Total Pages | : 222 |
Release | : 2006 |
Genre | : Medical |
ISBN | : 0763738433 |
A perfect accompaniment to any Human Biology course, Charles Welsh's Human Biology Laboratory Manual boasts 18 lab exercises aimed at educating students on how the human body works. Labs within the manual may be taught in any order, offering instructors the flexibility to cater the text to their own needs and course lengths.