Hormone Assays in Biological Fluids

Hormone Assays in Biological Fluids
Author: Michael J. Wheeler
Publisher: Springer Science & Business Media
Total Pages: 259
Release: 2008-02-04
Genre: Science
ISBN: 1592599869

Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can set up novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.

Hormone Assays in Biological Fluids

Hormone Assays in Biological Fluids
Author: Michael J. Wheeler
Publisher: Humana Press
Total Pages: 258
Release: 2006-01-16
Genre: Science
ISBN: 9781588290052

Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can set up novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.

Assay of Protein and Polypeptide Hormones

Assay of Protein and Polypeptide Hormones
Author: H. Van Cauwenberge
Publisher: Elsevier
Total Pages: 255
Release: 2014-05-17
Genre: Medical
ISBN: 1483145964

International Series of Monographs in Pure and Applied Biology, Modern Trends in Physiological Sciences, Volume 33: Assay of Protein and Polypeptide Hormones focuses on the assays of protein and polypeptide hormones. The selection first discusses the general principles of protein and polypeptide hormone assays, including conditions and basic factors for bioassays and status of biological assays. The book looks at the assays of hypophyseal hormones, such as growth hormones, adrenocorticotropin (ACTH), thyrotropin, gonadotropins, prolactin, vasopressin, and oxytocin. Concerns include radioimmunoassay, standards of thyrotropin, immunoassay of hypophyseal gonadotropins, and bioassay of prolactin. The book evaluates the assays of pancreatic hormones, such as insulin and glucagon. Chemical structure and properties of insulin and radioimmunoassay of glucagon are explained. The text also focuses on the assays of erythropoietin, renin, human chorionic gonadotropin, and human placental lactogen (HPL). Concerns include augmentation of red cell volume; biological application of renin assays; and similarities and differences between hypophyseal somatotropin and HPL. The selection is a valuable source of data for readers interested in the assays of protein and polypeptide hormones.

Hormone Assay

Hormone Assay
Author: C. W. Emmens
Publisher: Academic Press
Total Pages: 569
Release: 2013-10-22
Genre: Medical
ISBN: 1483272869

Hormone Assay considers assay methods in detail, focusing on vertebrate hormones. Statistics as applied to biological assay is another field in which recent advances have been phenomenal, although the application of modern methods to the design and analysis of many assays awaits full development. This simultaneous progress in the two fields of work has nevertheless provided a fruitful series of examples of how assays can be conducted in order to gain the greatest amount of information from the material available, and to express results in precise form. The book opens with a chapter on the use of and need for statistics in assays, followed by a chapter on insulin, which exemplifies some of the methods described. Subsequent chapters discuss assay methods for parathyroid hormone, adrenalin, pituitary lobe hormones, melanophore expanding hormone from the pituitary, gonadotropins, adrenocorticotropin, thyrotropic hormone, and lactogenic hormone. Also covered are assay procedures for mammogenic hormone, mammogenic hormone, androgens, and estrogens.

Oxford Textbook of Endocrinology and Diabetes

Oxford Textbook of Endocrinology and Diabetes
Author: John A.H. Wass
Publisher: Oxford University Press, USA
Total Pages: 2158
Release: 2011-07-28
Genre: Medical
ISBN: 0199235295

Now in its second edition, the Oxford Textbook of Endocrinology and Diabetes is a fully comprehensive, evidence-based, and highly-valued reference work combining basic science with clinical guidance, and providing first rate advice on diagnosis and treatment.