This report summarizes research documenting the 2015 harvests and uses of subsistence resources in 4 Interior Alaska communities located within the Nenana River Basin. Household surveys and ethnographic interviews were conducted in Anderson, Denali Park, Ferry, and Nenana in early 2016 in order to 1) estimate annual harvests and uses of wild foods in a 12-month study period; 2) map areas used for hunting, fishing, and gathering; 3) collect demographic, income, and food security information; 4) evaluate trends in wild resource harvests; and 5) document traditional knowledge observations regarding wild resources. Results indicate that salmon, large land mammals, vegetation, and nonsalmon fish composed the majority of wild food harvests by edible weight for these communities in 2015, but that residents of the study communities used over 100 different resources. Historical harvest data available from 1987 for 2 of the 4 communities--Anderson and Denali Park--show declines in the total and per capita pounds of subsistence foods harvested when compared to 2015, which is consistent with trends seen across much of Interior Alaska. Respondents searched for and harvested subsistence resources both adjacent to their communities and in a variety of areas throughout Interior and Southcentral Alaska. Many respondents gave resources to or received resources from other households within and outside their communities. In ethnographic interviews, key respondents shared their knowledge and experiences related to their harvests and uses of subsistence resources including patterns of seasonal harvests, methods of harvesting and processing, land use patterns, changes in the community over time, concerns for the management of fish and wildlife populations, and concerns regarding the proposed development of a LNG pipeline. This project was primarily funded by a reimbursable services agreement with the Alaska State Pipeline Coordinators Section. Additional funding was provided by the Alaska Gasline Development Corporation and Alaska Department of Fish and Game Division of Subsistence. The results of this research can assist agencies and other interested parties in understanding the ways in which subsistence resources are used within these communities. Results may be also used as part of the National Environmental Policy Act review of the proposed Alaska LNG Project alternatives.