Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
Author: James Pawley
Publisher: Springer Science & Business Media
Total Pages: 639
Release: 2013-04-17
Genre: Science
ISBN: 1475753489

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
Author: James B. Pawley
Publisher: Springer Science & Business Media
Total Pages: 672
Release: 1995
Genre: Medical
ISBN: 9780306448263

This newly updated second edition details the latest instrumentation and applications of the confocal microscope. This edition features 21 new chapters and includes information on preparing living specimens for the confocal microscope.

Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
Author: James Pawley
Publisher: Springer Science & Business Media
Total Pages: 234
Release: 2012-12-06
Genre: Medical
ISBN: 1461571332

In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.

Handbook of Biomedical Nonlinear Optical Microscopy

Handbook of Biomedical Nonlinear Optical Microscopy
Author: Barry R. Masters
Publisher: Oxford University Press
Total Pages: 895
Release: 2008-05-19
Genre: Science
ISBN: 0198036825

The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.

Cell Biological Applications of Confocal Microscopy

Cell Biological Applications of Confocal Microscopy
Author:
Publisher: Academic Press
Total Pages: 521
Release: 2003-01-03
Genre: Science
ISBN: 0080925359

This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures

Data Processing Handbook for Complex Biological Data Sources

Data Processing Handbook for Complex Biological Data Sources
Author: Gauri Misra
Publisher: Academic Press
Total Pages: 191
Release: 2019-03-23
Genre: Science
ISBN: 0128172800

Data Processing Handbook for Complex Biological Data provides relevant and to the point content for those who need to understand the different types of biological data and the techniques to process and interpret them. The book includes feedback the editor received from students studying at both undergraduate and graduate levels, and from her peers. In order to succeed in data processing for biological data sources, it is necessary to master the type of data and general methods and tools for modern data processing. For instance, many labs follow the path of interdisciplinary studies and get their data validated by several methods. Researchers at those labs may not perform all the techniques themselves, but either in collaboration or through outsourcing, they make use of a range of them, because, in the absence of cross validation using different techniques, the chances for acceptance of an article for publication in high profile journals is weakened. - Explains how to interpret enormous amounts of data generated using several experimental approaches in simple terms, thus relating biology and physics at the atomic level - Presents sample data files and explains the usage of equations and web servers cited in research articles to extract useful information from their own biological data - Discusses, in detail, raw data files, data processing strategies, and the web based sources relevant for data processing

Fluorescence Microscopy

Fluorescence Microscopy
Author: Ulrich Kubitscheck
Publisher: John Wiley & Sons
Total Pages: 508
Release: 2017-03-27
Genre: Medical
ISBN: 3527687726

Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.

Basic Methods in Microscopy

Basic Methods in Microscopy
Author: David L. Spector
Publisher: CSHL Press
Total Pages: 384
Release: 2006
Genre: Science
ISBN: 0879697512

This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.

Basic Confocal Microscopy

Basic Confocal Microscopy
Author: W. Gray (Jay) Jerome
Publisher: Springer
Total Pages: 371
Release: 2018-10-30
Genre: Science
ISBN: 3319974548

Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available.