Genotyping

Genotyping
Author: Stefan J. White
Publisher: Humana
Total Pages: 0
Release: 2016-11-08
Genre: Medical
ISBN: 9781493964406

This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.

Genotyping

Genotyping
Author: Ibrokhim Y. Abdurakhmonov
Publisher: Intechopen
Total Pages: 182
Release: 2018
Genre: Science
ISBN: 1789232805

A rare norovirus (NoV) genotype GII.17 has recently emerged and rapidly became predominant in most East Asian countries in the winters of 2014-2015. In this study, we report the diversity of NoV GII.17 in detail; a total of 646 GII.17 sequences obtained during 1978-2015 were analyzed and subjected to meta-analysis. At least five major recombinant GII.17 clusters were identified. Each recombinant variant group appeared to have emerged following the time order: GII.P4-GII.17 (1978-1990), GII.P16-GII.17 (2001-2004), GII.P13-GII.17 (2004-2010), GII.Pe-GII.17 (2012-2015) and GII.P3-GII.17 (2011-2015). The newly emerged GII.P3-GII.17 variant, which exhibited significant sequence and structure variations, is evolving toward a unique lineage. Our results indicate that circulation of GII.17 appears to change every 3-5 years due to replacement by a newly emerged variant and that the evolution of GII.17 is sequentially promoted by inter-genotype recombination, which contributes to the exchange between non-GII.17 and GII.17 RdRp genes and drives the evolution of GII.17 capsid genes.

Plant Genotyping

Plant Genotyping
Author: Robert J. Henry
Publisher: CABI
Total Pages: 352
Release: 2001-08-17
Genre: Law
ISBN: 9780851998930

Plant genotyping, or DNA fingerprinting of plants, is a technology that has matured and is poised for widespread practical application in the fields of breeding, commerce and research. This book examines the technologies available and their application in the analysis of:Wild plant populationsGermplasm collections Plant breedingContributors include leading research workers in this field from North America, Europe and Australasia.

Plant Genotyping

Plant Genotyping
Author: Yuri Shavrukov
Publisher: Springer Nature
Total Pages: 464
Release: 2023-02-13
Genre: Science
ISBN: 1071630245

This thorough volume presents a wide range of existing methods, from the very popular to the more exotic, in the area of plant genotyping. Many methods of plant genotyping were initially developed for medical research, but all genotyping methods, if they are to be successful, should be suitable for application across the full range of studies within plant biology, as seen in this collection. Plant genotyping methods herein are based on a variety of assessments, including DNA microarray, with its hundreds of thousands of simultaneous reactions, or separate individual studies of DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, digestion with restriction endonucleases, microscopy and many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Genotyping: Methods and Protocols offers researchers the opportunity to update their knowledge and choose the most suitable method of plant genotyping for their chosen application.

Plant Genotyping

Plant Genotyping
Author: Jacqueline Batley
Publisher:
Total Pages: 315
Release: 2015
Genre: Botany
ISBN: 9781493919666

The GenoType Diet

The GenoType Diet
Author: Dr. Peter J. D'Adamo
Publisher: Harmony
Total Pages: 354
Release: 2007-12-26
Genre: Health & Fitness
ISBN: 076792844X

What’s Your GenoType? GenoType 1 The Hunter Tall, thin, and intense, with an overabundance of adrenaline and a fierce, nervous energy that winds down with age, the Hunter was originally the success story of the human species. Vulnerable to systemic burnout when overstressed, the Hunter’s modern challenge is to conserve energy for the long haul. GenoType 2 The Gatherer Full-figured, even when not overweight, the Gatherer struggles with body image in a culture where thin is “in.” An unsuccessful crash dieter with a host of metabolic challenges, the Gatherer becomes a glowing example of health when properly nourished. GenoType 3 The Teacher Strong, sinewy, and stable, with great chemical synchronicity and stamina, the Teacher is built for longevity—given the right diet and lifestyle. This is the genotype of balance, blessed with a tremendous capacity for growth and fulfillment. GenoType 4 The Explorer Muscular and adventurous, the Explorer is a biological problem solver, with an impressive ability to adapt to environmental changes, and a better than average capacity for gene repair. The Explorer’s vulnerability to hormonal imbalances and chemical sensitivities can be overcome with a balanced diet and lifestyle. GenoType 5 The Warrior Long, lean, and healthy in youth, the Warrior is subject to a bodily rebellion in midlife.With the optimal diet and lifestyle, the Warrior can overcome the quick-aging metabolic genes and experience a second, “silver,” age of health. GenoType 6 The Nomad A GenoType of extremes, with a great sensitivity to environmental conditions—especially changes in altitude and barometric pressure, the Nomad is vulnerable to neuromuscular and immune problems. Yet a well-conditioned Nomad has the enviable gift of controlling caloric intake and aging gracefully. The author of the international bestseller Eat Right 4 Your Type again breaks new ground with the first diet plan based on your unique genetic code. With Eat Right 4 Your Type and additional books in the Blood Type Diet® series, Dr. Peter J. D’Adamo pioneered a new, revolutionary approach to dieting—one linked to a person’s blood type. In the GenoType Diet, he takes his groundbreaking research to the next level by identifying six unique genetic types. Whether you are a Hunter, Gatherer, Teacher, Explorer, Warrior, or Nomad, Dr. D’Adamo offers a customized program that compliments your genetic makeup to maximize health and weight loss, as well as prevent or even reverse disease. In simple, concise prose, Dr. D’Adamo explains how a host of environmental factors, including diet and lifestyle, dictate how and when your genes express themselves. He goes on to demonstrate precisely how, with the right tools, you can alter your genetic destiny by turning on the good genes and silencing the bad ones. Your health risks, weight, and life span can all be improved by following The GenoType Diet that’s right for you. Using family history and blood type, as well as simple diagnostic tools like fingerprint analysis, leg length measurements, and dental characteristics, Dr. D’Adamo shows you how to map out your genetic identity and discover which of the six GenoType plans you should follow. Without expensive tests or a visit to the doctor, The GenoType Diet reveals previously hidden genetic strengths and weaknesses and provides a precise diet and lifestyle plan for every individual. Based on the latest and most cutting-edge genetic research, this is a twenty-first-century plan for wellness and weight loss from a renowned healthcare pioneer.

Genotyping by Sequencing for Crop Improvement

Genotyping by Sequencing for Crop Improvement
Author: Humira Sonah
Publisher: John Wiley & Sons
Total Pages: 404
Release: 2022-03-28
Genre: Science
ISBN: 1119745659

OGENOTYPING BY SEQUENCING FOR CROP IMPROVEMENT A thoroughly up-to-date exploration of genotyping-by-sequencing technologies and related methods in plant science In Genotyping by Sequencing for Crop Improvement, a team of distinguished researchers delivers an in-depth and current exploration of the latest advances in genotyping-by-sequencing (GBS) methods, the statistical approaches used to analyze GBS data, and its applications, including quantitative trait loci (QTL) mapping, genome-wide association studies (GWAS), and genomic selection (GS) in crop improvement. This edited volume includes insightful contributions on a variety of relevant topics, like advanced molecular markers, high-throughput genotyping platforms, whole genome resequencing, QTL mapping with advanced mapping populations, analytical pipelines for GBS analysis, and more. The distinguished contributors explore traditional and advanced markers used in plant genotyping in extensive detail, and advanced genotyping platforms that cater to unique research purposes are discussed, as is the whole-genome resequencing (WGR) methodology. The included chapters also examine the applications of these technologies in several different crop categories, including cereals, pulses, oilseeds, and commercial crops. Genotyping by Sequencing for Crop Improvement also offers: A thorough introduction to molecular marker techniques and recent advancements in the technology Comprehensive explorations of the genotyping of seeds while preserving their viability, as well as advances in genomic selection Practical discussions of opportunities and challenges relating to high throughput genotyping in polyploid crops In-depth examinations of recent advances and applications of GBS, GWAS, and GS in cereals, pulses, oilseeds, millets, and commercial crops Perfect for practicing plant scientists with an interest in genotyping-by-sequencing technology, Genotyping by Sequencing for Crop Improvement will also earn a place in the libraries of researchers and students seeking a one-stop reference on the foundational aspects of – and recent advances in – genotyping-by-sequencing, genome-wide association studies, and genomic selection.

Plant Genotyping: From Traditional Markers to Modern Technologies

Plant Genotyping: From Traditional Markers to Modern Technologies
Author: Yuri Shavrukov
Publisher: Frontiers Media SA
Total Pages: 201
Release: 2024-05-09
Genre: Science
ISBN: 2832548911

In contrast to the external traits of plants, we cannot directly see the genotypes that comprise the underlying set of genetic material encoding these phenotypic traits. To make genotypes accessible for research and further understanding, various genotyping methods are used. Plant genotyping began with relatively simple and elementary molecular markers, like microsatellites or SSR (Simple Sequence Repeats), which were then followed by DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, and now today’s modern and advanced microchip-DNA technology involving hundreds and thousands of reactions simultaneously.

Plant Genotyping II

Plant Genotyping II
Author: Robert J. Henry
Publisher: CABI
Total Pages: 295
Release: 2008
Genre: Technology & Engineering
ISBN: 1845933826

This book aims to describe some of the important recent developments in plant genotyping. It is based upon a second workshop held recently to review progress in this area. Recent developments focus on high- throughput methods and generally target single nucleotide polymorphism (SNP) discovery and analysis. The topics covered include: SNP discovery in plants; SNPs and their use in maize; rare SNP discovery with endonucleases; sequence polymorphisms in the flanking regions of microsatellite markers; SNP discovery by ecotilling using capillary electrophoresis; genotyping by allele-specific PCR; the MassARRAY system for plant genomics; mutation screening; nanotechnology (the future of cost-effective plant genotyping); functionally associated molecular genetic markers for temperate pasture plant improvement; genotyping for rice eating qualities; towards universal loci for plant genotyping; DNA banks as a resource for SNP genotyping; DNA extraction from plant tissue; future prospects for plant genotyping.

Advances and Applications of Cost-Effective, High-Throughput Genotyping Technologies for Sustainable Agriculture

Advances and Applications of Cost-Effective, High-Throughput Genotyping Technologies for Sustainable Agriculture
Author: Nisha Singh
Publisher: Frontiers Media SA
Total Pages: 196
Release: 2023-12-28
Genre: Science
ISBN: 2832541860

Recent advances in next-generation sequencing-driven mass production of genomic data and various other integrated techniques have considerably broadened and deepened our understanding of living organisms' molecular systems. Because complex quantitative traits are difficult to select due to low heritability, conventional plant breeding relies on phenotypic selection and breeder experience, it takes longer to develop a new, improved variety. For association studies to identify DNA markers linked to these complex traits, genotyping chip arrays allow genotyping of thousands of markers in a short amount of time. Plant breeding consistency and predictability have improved thanks to advances in genomics. NGS technologies bring new tools and concepts that can enhance the precision and efficiency of plant breeding such as cost-effective, high throughput genotyping technologies for sustainable agriculture. These genotyping technologies will be lowering the time and cost of developing high-quality food crops that are stress-resistant while still having a high nutritional value. This Research Topic focuses on recent advancements in NGS-related technologies, mainly the development of cost-effective high-throughput genotyping platforms with a wide range of bioinformatics tools, and possible translational multi-omics applications in crop breeding programs for sustainable agriculture.