Genomics Aided Development And Characterization Of Cucumis Hystrix Introgression Lines In Cucumber
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Author | : Paradee Thammapichai |
Publisher | : |
Total Pages | : 0 |
Release | : 2019 |
Genre | : |
ISBN | : |
Cucumis hystrix (2n = 2x = 24) is the only known species in the genus Cucumis that is cross-compatible with cucumber (C. sativus, 2n = 2x = 14) and has potentials for cucumber improvement. To facilitate introgression of C. hystrix chromatins into cucumber genetic background, we developed a draft genome assembly for C. hystrix (TH1 accession) which contained 16,865 scaffolds (~78x coverage) for a total of 226.0 Mb, representing ~51% of the estimated 447 Mb C. hystrix genome. Through genotyping-by-sequencing (GBS), a linkage map for C. hystrix was constructed with 1,692 SNP loci, which was then integrated with a previously developed genetic map with 410 SSR markers. The resulting consensus map consisted of 12 linkage groups spanning 1119 cM and anchor 1,321 scaffolds, accounting for 60.2 Mb or ~26.62% of C. hystrix draft genome assembly. A karyotype for the C. hystrix genome was developed with molecular cytogenetic landmarks which allowed for a better understanding of the syntenic relationships among C. hystrix, cucumber and melon (C. melo, 2n = 2x = 24) chromosomes as well as the mechanisms in which the seven cucumber chromosomes were evolved from an x=12 ancestor through dysploid chromosome reduction. C. hystrix introgression lines (IL) in elite cucumber backgrounds were developed through marker-assisted backcrossing. Overall, 334 ILs were identified by SSR markers to carry C. hystrix alleles. These ILs included different introgression segments that covered about 57.29% of C. hystrix map. With genotyping-by-sequencing (GBS), the introgressions in the 55 ILs included 44 syntenic blocks or about 74.57% coverage of C. hystrix genetic map. Each IL has an average 5.4 introgression segments, averaging 1.7 Mbp in size. Homoeologous recombination reduction was observed genome-wide but recombination hotspots in C. hystrix were also identified. Phenotypic characterization for resistance to angular leaf spot (ALS) and downy mildew (DM) pathogens was conducted in selected ILs. Both genotypic data and phenotypic data indicated that the intermediate resistance to ALS in nine ILs and the high resistance to DM in four ILs was of C. hystrix origin. These C. hystrix introgression lines, although time consuming in development, should be valuable resources for cucumber improvement.
Author | : Nitish Kumar |
Publisher | : Springer Nature |
Total Pages | : 367 |
Release | : 2023-06-29 |
Genre | : Technology & Engineering |
ISBN | : 9819916127 |
This edited book covers the applications of molecular markers in the genetic improvement of crop plants. Recent advances in molecular marker techniques such as the development of high-throughput genotyping platforms, marker-assisted selection, and non-coding RNA-based markers have been discussed. Essential information is provided on functional markers, genotype-by-sequencing, and association mapping methodologies that can facilitate accelerated crop breeding programs for increased yield, high nutritional quality, and tolerance to a variety of abiotic and biotic stresses. This volume presents basic information on molecular marker techniques from marker location up to gene cloning. The book includes a description of technical approaches in genome analysis such as comparison of marker systems, positional cloning, and array techniques. This book is of interest to teachers, researchers, and plant breeders. The book also serves as additional reading material for undergraduate and graduate students of agriculture, horticulture, and forestry.
Author | : Chittaranjan Kole |
Publisher | : Springer Science & Business Media |
Total Pages | : 304 |
Release | : 2011-08-17 |
Genre | : Science |
ISBN | : 3642204503 |
Wild crop relatives are now playing a significant part in the elucidation and improvement of the genomes of their cultivated counterparts. This work includes comprehensive examinations of the status, origin, distribution, morphology, cytology, genetic diversity and available genetic and genomic resources of numerous wild crop relatives, as well as of their evolution and phylogenetic relationship. Further topics include their role as model plants, genetic erosion and conservation efforts, and their domestication for the purposes of bioenergy, phytomedicines, nutraceuticals and phytoremediation. Wild Crop Relatives: Genomic and Breeding Resources comprises 10 volumes on Cereals, Millets and Grasses, Oilseeds, Legume Crops and Forages, Vegetables, Temperate Fruits, Tropical and Subtropical Fruits, Industrial Crops, Plantation and Ornamental Crops, and Forest Trees. It contains 125 chapters written by nearly 400 well-known authors from about 40 countries.
Author | : Rebecca Grumet |
Publisher | : Springer |
Total Pages | : 434 |
Release | : 2017-10-13 |
Genre | : Science |
ISBN | : 3319493329 |
This book provides an overview of the current state of knowledge of the genetics and genomics of the agriculturally important Cucurbitaceae plant family, which includes crops such as watermelon, melon, cucumber, summer and winter squashes, pumpkins, and gourds. Recent years have resulted in tremendous increases in our knowledge of these species due to large scale genomic and transcriptomic studies and production of draft genomes for the four major species, Citrullus lanatus, Cucumis melo, Cucumis sativus, and Cucurbita spp. This text examines genetic resources and structural and functional genomics for each species group and across species groups. In addition, it explores genomic-informed understanding and commonalities in cucurbit biology with respect to vegetative growth, floral development and sex expression, fruit growth and development, and important fruit quality traits.
Author | : Food and Agriculture Organization of the United Nations |
Publisher | : Food & Agriculture Org. |
Total Pages | : 576 |
Release | : 2019-03-12 |
Genre | : Political Science |
ISBN | : 9251312702 |
The State of the World's Biodiversity for Food and Agriculture presents the first global assessment of biodiversity for food and agriculture worldwide. Biodiversity for food and agriculture is the diversity of plants, animals and micro-organisms at genetic, species and ecosystem levels, present in and around crop, livestock, forest and aquatic production systems. It is essential to the structure, functions and processes of these systems, to livelihoods and food security, and to the supply of a wide range of ecosystem services. It has been managed or influenced by farmers, livestock keepers, forest dwellers, fish farmers and fisherfolk for hundreds of generations. Prepared through a participatory, country-driven process, the report draws on information from 91 country reports to provide a description of the roles and importance of biodiversity for food and agriculture, the drivers of change affecting it and its current status and trends. It describes the state of efforts to promote the sustainable use and conservation of biodiversity for food and agriculture, including through the development of supporting policies, legal frameworks, institutions and capacities. It concludes with a discussion of needs and challenges in the future management of biodiversity for food and agriculture. The report complements other global assessments prepared under the auspices of the Commission on Genetic Resources for Food and Agriculture, which have focused on the state of genetic resources within particular sectors of food and agriculture.
Author | : Pradeep Kumar |
Publisher | : Springer |
Total Pages | : 471 |
Release | : 2016-04-28 |
Genre | : Science |
ISBN | : 3319273124 |
Plant diseases play an important role on our daily lives. Most of plant diseases are visible and are caused by biotic and/or abiotic factors. Symptoms are usually the results of a morphological change, alteration or damage to plant tissue and/or cells due to an interference of the plant’s metabolism. All basic structures of vascular plants are subject to attack by pathogens. The failure in accurate disease diagnosis and management may lead to huge losses in plant production and related commodities, which causes nutritional food scarcity. Typically, the appearance of a biotic symptom will indicate the relatively late stage of an infection and/or colonization of a pathogen. Expert detection, accurate diagnosis, and timely management play a significant role in keeping plants free from pathogens. In this book expert scholars share their research knowledge and key literature which are vital toward the diagnosis of plant diseases across the globe, addressing traditional plant pathology techniques, as well as advanced molecular diagnostic approach.
Author | : P.T.N. Spencer-Phillips |
Publisher | : Springer Science & Business Media |
Total Pages | : 272 |
Release | : 2007-05-08 |
Genre | : Science |
ISBN | : 0306479141 |
P. T. N. SPENCER-PHILLIPS Co-ordinator, Downy Mildew Working Group of the International Society for Plant Pathology University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK Email: [email protected] It is a very great privilege to write the preface to the first specialist book on downy mildews since the major work edited by D. M. Spencer in 1981. The idea for the present publication arose from the Downy Mildew Workshop at the International Congress of Plant Pathology (ICPP) held in Edinburgh in August 1998. Our intention was to invite reviews on selected aspects of downy mildew biology from international authorities, and link these to a series of related short contributions reporting new data. No attempt has been made to cover the breadth of downy mildew research, but we hope that further topics will be included in future volumes, so that this becomes the first of a series following the five year ICPP cycle.
Author | : Satbir Singh Gosal |
Publisher | : Springer Nature |
Total Pages | : 455 |
Release | : 2020-05-23 |
Genre | : Science |
ISBN | : 3030418669 |
Plant improvement has shifted its focus from yield, quality and disease resistance to factors that will enhance commerical export, such as early maturity, shelf life and better processing quality. Conventional plant breeding methods aiming at the improvement of a self-pollinating crop, such as wheat, usually take 10-12 years to develop and release of the new variety. During the past 10 years, significant advances have been made and accelerated methods have been developed for precision breeding and early release of crop varieties. This work summarizes concepts dealing with germplasm enhancement and development of improved varieties based on innovative methodologies that include doubled haploidy, marker assisted selection, marker assisted background selection, genetic mapping, genomic selection, high-throughput genotyping, high-throughput phenotyping, mutation breeding, reverse breeding, transgenic breeding, shuttle breeding, speed breeding, low cost high-throughput field phenotyping, etc. It is an important reference with special focus on accelerated development of improved crop varieties.
Author | : Gyana Ranjan Rout |
Publisher | : Academic Press |
Total Pages | : 664 |
Release | : 2022-07-16 |
Genre | : Science |
ISBN | : 0323899137 |
Omics in Horticulture Crops presents a comprehensive view of germplasm diversity, genetic evolution, genomics, proteomics and transcriptomics of fruit crops (temperate, tropical and subtropical fruits, fruit nuts, berries), vegetables, tuberous crops, ornamental and floricultural crops and medicinal aromatic plants. Information covering phenomics, genetic diversity, phylogenetic studies, genome sequencing, and genome barcoding through the utilization of molecular markers plays an imperative role in the characterization and effective utilization of diverse germplasm are included in the book. This is a valuable reference for researchers and academics seeking to improve cultivar productivity through enhanced genetic diversity while also retaining optimal traits and protecting the growing environment. Highlights perspectives, progress and promises of -omics application Provides a systematic overview of origin, progenitor and domestication process as well as genetic insights Includes full range of horticultural crops
Author | : Sudhakar Pandey |
Publisher | : Springer |
Total Pages | : 0 |
Release | : 2022-12-31 |
Genre | : Science |
ISBN | : 9783030886493 |
This edited book presents the latest research on cucumber, its genetic resources and diversity, tissue culture and genetic transformation, mapping of economic genes and QTLs, whole genome sequencing, comparative genomics, and breeding strategies. The mechanism of sex expression, interspecific hybridization, and cell biology are also described. The book discusses the genome draft of cucumber and the application of genome editing. This book is useful to the students, teachers and scientists in academia and relevant private companies interested in horticulture, genetics, breeding, and related areas.