Fundamental Laboratory Approaches For Biochemistry And Biotechnology
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Author | : Alexander J. Ninfa |
Publisher | : John Wiley & Sons |
Total Pages | : 480 |
Release | : 2009-05-26 |
Genre | : Science |
ISBN | : 0470087668 |
Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.
Author | : Lisa A. Seidman |
Publisher | : CRC Press |
Total Pages | : 1210 |
Release | : 2021-12-29 |
Genre | : Medical |
ISBN | : 1000480763 |
Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout. Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students’ progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments. Key Features: Provides a valuable reference for laboratory professionals at all stages of their careers. Focuses on basic principles and methods to provide students with the knowledge needed to begin a career in the Biotechnology industry. Describes fundamental laboratory skills. Includes laboratory scenario-based questions that require students to write or discuss their answers to ensure they have mastered the chapter content. Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. Tables, a detailed glossary, practice problems and solutions, case studies and anecdotes provide students with the tools needed to master the content.
Author | : Firdos Alam Khan |
Publisher | : CRC Press |
Total Pages | : 694 |
Release | : 2015-11-18 |
Genre | : Medical |
ISBN | : 1498723446 |
A single source reference covering every aspect of biotechnology, Biotechnology Fundamentals, Second Edition breaks down the basic fundamentals of this discipline, and highlights both conventional and modern approaches unique to the industry. In addition to recent advances and updates relevant to the first edition, the revised work also covers ethics in biotechnology and discusses career possibilities in this growing field. The book begins with a basic introduction of biotechnology, moves on to more complex topics, and provides relevant examples along the way. Each chapter begins with a brief summary, is illustrated by simple line diagrams, pictures, and tables, and ends with a question session, an assignment, and field trip information. The author also discusses the connection between plant breeding, cheese making, in vitro fertilization, alcohol fermentation, and biotechnology. Comprised of 15 chapters, this seminal work offers in-depth coverage of topics that include: Genes and Genomics Proteins and Proteomics Recombinant DNA Technology Microbial Biotechnology Agricultural Biotechnology Animal Biotechnology Environmental Biotechnology Medical Biotechnology Nanobiotechnology Product Development in Biotechnology Industrial Biotechnology Ethics in Biotechnology Careers in Biotechnology Laboratory Tutorials Biotechnology Fundamentals, Second Edition provides a complete introduction of biotechnology to students taking biotechnology or life science courses and offers a detailed overview of the fundamentals to anyone in need of comprehensive information on the subject.
Author | : Tai Te Wu |
Publisher | : Springer Science & Business Media |
Total Pages | : 266 |
Release | : 2007-05-08 |
Genre | : Science |
ISBN | : 0306468921 |
Analytical Molecular Biology illustrates the importance of simple analytical methods applied to some basic molecular biology problems, with an emphasis on the importance of biological problems, rather than the complexity of mathematics. First, the book examines crucial experimental data for a specific problem. Mathematical models will then be constructed with explicit inclusion of biological facts. From such models, predictions can be deduced and then suggest further experimental studies. A few important molecular biology problems will be discussed in the order of the complexity of the mathematical models. Based on such illustrations, the readers can then develop their own analytical methods to study their own problems. This book is for anyone who knows they need to learn how to apply mathematical models to biology, but doesn't necessarily want to, from practicing researchers looking to acquire more analytical tools to advanced students seeking a clear, explanatory text.
Author | : Christopher Bucke |
Publisher | : Springer Science & Business Media |
Total Pages | : 334 |
Release | : 2008-02-05 |
Genre | : Science |
ISBN | : 1592592619 |
We are in a phase of the evolution of biotechnology in which the true and potential commercial importance of carbohydrates is becoming appre- ated more fully. Progress in providing hard facts to establish the commercial value ofpolysaccharides and oligosaccharides is limited, as always, by lack of funding and by a relative shortage of skilled practitioners in the production and analysis of those materials. Carbohydrate science has a reputation, not unmerited, for technical difficulty owing to the structural similarity of the many monosaccharide monomers and the potential, and real, complexity of oligosaccharides and polysaccharides, particularly heterosaccharides conta- ing many different monomers. Modem analytical and synthetic methods, in many cases using enzyme technology, are beginning to allow this complexity to be unraveled. Carbohydrate Biotechnology Protocols is aimed at those newcomers who have an interest in the production and use of carbohydrate materials, but have shied away from involvement for lack of detailed descriptions of appropriate methods, including the type of practical hints that may be provided by those skilled in those methods, but that are rarely described in research papers. The majority of the contributions to this book conform to the established format of the Methods in Biotechnology series. They begin with the theoretical and c- mercial background to the method or group of methods, provide a list of the reagents and equipment required for the procedure, then give a detailed st- by-step description of how to carry out the protocol.
Author | : Thomas Nogrady |
Publisher | : Oxford University Press |
Total Pages | : 664 |
Release | : 2005-08-11 |
Genre | : Medical |
ISBN | : 0190282967 |
Fully updated and rewritten by a basic scientist who is also a practicing physician, the third edition of this popular textbook remains comprehensive, authoritative and readable. Taking a receptor-based, target-centered approach, it presents the concepts central to the study of drug action in a logical, mechanistic way grounded on molecular and principles. Students of pharmacy, chemistry and pharmacology, as well as researchers interested in a better understanding of drug design, will find this book an invaluable resource. Starting with an overview of basic principles, Medicinal Chemistry examines the properties of drug molecules, the characteristics of drug receptors, and the nature of drug-receptor interactions. Then it systematically examines the various families of receptors involved in human disease and drug design. The first three classes of receptors are related to endogenous molecules: neurotransmitters, hormones and immunomodulators. Next, receptors associated with cellular organelles (mitochondria, cell nucleus), endogenous macromolecules (membrane proteins, cytoplasmic enzymes) and pathogens (viruses, bacteria) are examined. Through this evaluation of receptors, all the main types of human disease and all major categories of drugs are considered. There have been many changes in the third edition, including a new chapter on the immune system. Because of their increasingly prominent role in drug discovery, molecular modeling techniques, high throughput screening, neuropharmacology and genetics/genomics are given much more attention. The chapter on hormonal therapies has been thoroughly updated and re-organized. Emerging enzyme targets in drug design (e.g. kinases, caspases) are discussed, and recent information on voltage-gated and ligand-gated ion channels has been incorporated. The sections on antihypertensive, antiviral, antibacterial, anti-inflammatory, antiarrhythmic, and anticancer drugs, as well as treatments for hyperlipidemia and peptic ulcer, have been substantially expanded. One new feature will enhance the book's appeal to all readers: clinical-molecular interface sections that facilitate understanding of the treatment of human disease at a molecular level.
Author | : Peter J. Cragg |
Publisher | : Springer Science & Business Media |
Total Pages | : 267 |
Release | : 2010-08-26 |
Genre | : Science |
ISBN | : 9048125820 |
The aim of this book is to return to the biomimicry and medicinal potential that inspired many of the early supramolecular chemists and to set it in the context of current advances in the field. Following an overview of supramolecular chemistry, the first section considers the efforts made to synthesize artificial systems that mimic biological entities. The second section addresses the application of supramolecular principles to molecular diagnostics with a particular emphasis on the ‘receptor-relayreporter’ motif. Many of the examples chosen have clinical importance. The third section takes the clinical diagnostic theme further and demonstrates the therapeutic applications of supramolecular chemistry through photodynamic therapy, drug delivery, and the potential for synthetic peptides to form antibiotic tubes. The short epilogue considers the potential for supramolecular solutions to be found for further challenges in biomimetic and therapeutic chemistry.
Author | : R. Cecil Jack |
Publisher | : OUP USA |
Total Pages | : 376 |
Release | : 1995-05-25 |
Genre | : Computers |
ISBN | : 9780195078978 |
This book reviews the theoretical basis for many biophysical chemistry techniques commonly used in the biochemistry laboratory, and emphasizes the usefulness of computer spreadsheets in solving quantitative problems related to these methods.
Author | : Stefan Hohmann |
Publisher | : Springer Science & Business Media |
Total Pages | : 480 |
Release | : 2000 |
Genre | : Medical |
ISBN | : 9780306465017 |
Biophysical studies in the 1950ies and 1960ies led to the realization that the water permeability of certain biological membranes must be due to the presence of water transporting proteins. This hypothesis was confirmed in 1991 and 1992 with the pioneering discovery of the first molecular membrane water channel, CHIP28, by Agre and coworkers. This integral membrane protein, which is abundant in the erythrocyte membrane and in many epithelial cells, is now called aquaporin-1 or AQP1. Thus the terms water channel or aquaporin are synonymous. In July 2000 more than 200 researchers came together in Gothenburg, Sweden, for the `3rd International Conference on the Molecular Biology and Physiology of Water and Solute Transport" to discuss progress in this emerging research field. 58 different presentations from this conference are the basis for this book. Cumulatively, these 58 short chapters provide a balanced overview complementing numerous recent reviews in this field.
Author | : Martin Kenney |
Publisher | : Yale University Press |
Total Pages | : 328 |
Release | : 1986-01-01 |
Genre | : Science |
ISBN | : 0300033923 |