Focussed Issue Single Molecule Biophysics And Molecular Biology
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An Introduction to Single Molecule Biophysics
Author | : Yuri L. Lyubchenko |
Publisher | : CRC Press |
Total Pages | : 240 |
Release | : 2017-11-22 |
Genre | : Science |
ISBN | : 1351333127 |
This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.
Handbook of Single-Molecule Biophysics
Author | : Peter Hinterdorfer |
Publisher | : Springer Science & Business Media |
Total Pages | : 634 |
Release | : 2009-12-24 |
Genre | : Science |
ISBN | : 0387764976 |
This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Biology at the Single Molecule Level
Author | : S.H. Leuba |
Publisher | : Elsevier |
Total Pages | : 263 |
Release | : 2001-12-17 |
Genre | : Science |
ISBN | : 008056061X |
This is the first book solely devoted to single-molecule biochemistry and molecular biology. Authors were selected on the basis of their contribution to this new and exciting field, and were asked to focus more on the biological problems that can be approached using single-molecule techniques rather than on the techniques per se. It is thought that such techniques will eventually dominate the physical characterization of biologically important macromolecules.
Single-Molecule Biophysics
Author | : Tamiki Komatsuzaki |
Publisher | : John Wiley & Sons |
Total Pages | : 403 |
Release | : 2011-11-16 |
Genre | : Science |
ISBN | : 111813138X |
Discover the experimental and theoretical developments in optical single-molecule spectroscopy that are changing the ways we think about molecules and atoms The Advances in Chemical Physics series provides the chemical physics field with a forum for critical, authoritative evaluations of advances in every area of the discipline. This latest volume explores the advent of optical single-molecule spectroscopy, and how atomic force microscopy has empowered novel experiments on individual biomolecules, opening up new frontiers in molecular and cell biology and leading to new theoretical approaches and insights. Organized into two parts—one experimental, the other theoretical—this volume explores advances across the field of single-molecule biophysics, presenting new perspectives on the theoretical properties of atoms and molecules. Single-molecule experiments have provided fresh perspectives on questions such as how proteins fold to specific conformations from highly heterogeneous structures, how signal transductions take place on the molecular level, and how proteins behave in membranes and living cells.This volume is designed to further contribute to the rapid development of single-molecule biophysics research. Filled with cutting-edge research reported in a cohesive manner not found elsewhere in the literature, each volume of the Advances in Chemical Physics series serves as the perfect supplement to any advanced graduate class devoted to the study of chemical physics.
Single-Molecule Cellular Biophysics
Author | : Mark C. Leake |
Publisher | : Cambridge University Press |
Total Pages | : 291 |
Release | : 2013-01-31 |
Genre | : Science |
ISBN | : 1107005833 |
Indispensable textbook for undergraduate students in the physical and life sciences, unravelling the inner workings of the cell.
Single Molecule Biophysics and Poisson Process Approach to Statistical Mechanics
Author | : Susanta K Sarkar |
Publisher | : Morgan & Claypool Publishers |
Total Pages | : 74 |
Release | : 2016-04-01 |
Genre | : Science |
ISBN | : 1681741164 |
This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of science.
Single Molecule Cellular Biophysics
Author | : Royal Society (Great Britain) |
Publisher | : |
Total Pages | : |
Release | : 2012 |
Genre | : Cytology |
ISBN | : 9780854039906 |