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| Author | : S. Rajan |
| Publisher | : CBS Publishers & Distributors Pvt Limited, India |
| Total Pages | : |
| Release | : 2018-04-30 |
| Genre | : Medical |
| ISBN | : 9789386478252 |
This is a manual for all life science students studying courses in biochemistry, biotechnology, botany, genetics, microbiology, molecular biology, zoology, nursing, and medicine, based on the author's decades-long experience in the field experiments of life sciences teaching and research.
| Author | : Graeme D. Ruxton |
| Publisher | : Oxford University Press, USA |
| Total Pages | : 188 |
| Release | : 2006 |
| Genre | : Language Arts & Disciplines |
| ISBN | : |
Experimental Design for the Life Sciences teaches the reader how to effectively design experiments, to ensure that today's students are equipped with the skills they need to be the researchers of tomorrow. With a refreshingly approachable and articulate style, the book explains the essential elements of experimental design in clear, practical terms, so that the reader can grasp and apply even the most challenging concepts, including power analysis and pseudoreplication.
| Author | : Javeed Ahmad Tantray |
| Publisher | : Elsevier |
| Total Pages | : 232 |
| Release | : 2022-09-11 |
| Genre | : Science |
| ISBN | : 0443191751 |
Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource. Provides a practical resource on designing, executing and analyzing experiments and analytical procedures Includes detailed and standardized coverage of basic research methods in the area Presents step-by-step instructions on how to execute a large selection of life sciences experiments
| Author | : Prakash Singh Bisen |
| Publisher | : CRC Press |
| Total Pages | : 1812 |
| Release | : 2014-02-26 |
| Genre | : Medical |
| ISBN | : 1466553146 |
As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.
| Author | : Gauri Singhal |
| Publisher | : Cambridge Scholars Publishing |
| Total Pages | : 172 |
| Release | : 2022-09-15 |
| Genre | : Science |
| ISBN | : 152758870X |
Aimed at both undergraduate and postgraduate students, this practical handbook is the result of cooperative effort and is designed to meet the present needs of students. Clear and concise, it is prepared in accordance with the latest syllabi and guidelines, and explores the instruments, glassware, and plastic wares that are handled during experimental procedures and related information concerning calculations required to prepare chemical reagents and media.
| Author | : Morteza Jalali |
| Publisher | : Academic Press |
| Total Pages | : 383 |
| Release | : 2017-03-31 |
| Genre | : Science |
| ISBN | : 012803078X |
Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
| Author | : Oliviero Carugo |
| Publisher | : Humana |
| Total Pages | : 407 |
| Release | : 2016-08-23 |
| Genre | : Science |
| ISBN | : 9781493956883 |
Most life science researchers will agree that biology is not a truly theoretical branch of science. The hype around computational biology and bioinformatics beginning in the nineties of the 20th century was to be short lived (1, 2). When almost no value of practical importance such as the optimal dose of a drug or the three-dimensional structure of an orphan protein can be computed from fundamental principles, it is still more straightforward to determine them experimentally. Thus, experiments and observationsdogeneratetheoverwhelmingpartofinsightsintobiologyandmedicine. The extrapolation depth and the prediction power of the theoretical argument in life sciences still have a long way to go. Yet, two trends have qualitatively changed the way how biological research is done today. The number of researchers has dramatically grown and they, armed with the same protocols, have produced lots of similarly structured data. Finally, high-throu- put technologies such as DNA sequencing or array-based expression profiling have been around for just a decade. Nevertheless, with their high level of uniform data generation, they reach the threshold of totally describing a living organism at the biomolecular level for the first time in human history. Whereas getting exact data about living systems and the sophistication of experimental procedures have primarily absorbed the minds of researchers previously, the weight increasingly shifts to the problem of interpreting accumulated data in terms of biological function and bio- lecular mechanisms.
| Author | : National Research Council |
| Publisher | : National Academies Press |
| Total Pages | : 136 |
| Release | : 2007-11-10 |
| Genre | : Political Science |
| ISBN | : 0309111919 |
Based on a series of regional meetings on university campuses with officials from the national security community and academic research institutions, this report identifies specific actions that should be taken to maintain a thriving scientific research environment in an era of heightened security concerns. Actions include maintaining the open exchange of scientific information, fostering a productive environment for international scholars in the U.S., reexamining federal definitions of sensitive but unclassified research, and reviewing policies on deemed export controls. The federal government should establish a standing entity, preferably a Science and Security Commission, that would review policies regarding the exchange of information and the participation of foreign-born scientists and students in research.
| Author | : National Academies of Sciences, Engineering, and Medicine |
| Publisher | : National Academies Press |
| Total Pages | : 257 |
| Release | : 2019-10-20 |
| Genre | : Science |
| ISBN | : 0309486165 |
One of the pathways by which the scientific community confirms the validity of a new scientific discovery is by repeating the research that produced it. When a scientific effort fails to independently confirm the computations or results of a previous study, some fear that it may be a symptom of a lack of rigor in science, while others argue that such an observed inconsistency can be an important precursor to new discovery. Concerns about reproducibility and replicability have been expressed in both scientific and popular media. As these concerns came to light, Congress requested that the National Academies of Sciences, Engineering, and Medicine conduct a study to assess the extent of issues related to reproducibility and replicability and to offer recommendations for improving rigor and transparency in scientific research. Reproducibility and Replicability in Science defines reproducibility and replicability and examines the factors that may lead to non-reproducibility and non-replicability in research. Unlike the typical expectation of reproducibility between two computations, expectations about replicability are more nuanced, and in some cases a lack of replicability can aid the process of scientific discovery. This report provides recommendations to researchers, academic institutions, journals, and funders on steps they can take to improve reproducibility and replicability in science.
| Author | : Deepak Dwivedi |
| Publisher | : LAP Lambert Academic Publishing |
| Total Pages | : 68 |
| Release | : 2013 |
| Genre | : |
| ISBN | : 9783659455803 |
The Experimental protocols of Immunology & Molecular Biology are presented so as to be readily used at the laboratory bench. Although a number of the procedures described represent the tried and trusted, we have striven to include variants on existing technologies that an experiment can be performed. These step-by-step protocols are intended to be concise and easy to follow. Suggestions to successfully apply the procedures are included, along with recommended materials. A special feature is that, in addition to the protocols, important background information and representative results of applying the methods are given. The aim of this book to provide a self-contained laboratory manual which will be useful to Graduate, Post Graduates & Research Scholars of Life sciences of various universities and colleges.
