Evaluation and Application of Enteric Virus Recovery Techniques for Environmental Water Monitoring

Evaluation and Application of Enteric Virus Recovery Techniques for Environmental Water Monitoring
Author: Annie Rebecca Phillips
Publisher:
Total Pages: 128
Release: 2016
Genre:
ISBN:

Virus recovery techniques must preserve enteric viruses present in environmental waters and remove co-concentrated PCR inhibitory compounds. The aim of this study was to evaluate and apply virus recovery techniques for use in environmental water monitoring. For each technique, the percent recovery was determined for multiple enteric viral pathogens and process control viruses. Poly-ethylene glycol precipitation was shown to be the main source of viral loss, with percent recoveries ranging from 0.0% to 4.7%. Chloroform extraction and ultrafiltration had percent recoveries ranging from 277.2% to 0.6% and 62.5% to 9.7% respectively. The ultrafiltration technique was the most effective at removing PCR inhibitory compounds. Stream samples from Gwinnett County, Georgia were concentrated using the combined methods to test for multiple human enteric viral pathogens, with one sample testing positive for Sapovirus. This study highlights the need for more efficient recovery techniques to avoid underestimating possible public health risks.

Poliovirus and Rotavirus Detection in Water

Poliovirus and Rotavirus Detection in Water
Author: Jill Christin Falman
Publisher:
Total Pages: 47
Release: 2017
Genre:
ISBN:

Human enteric viruses are responsible for the majority of acute waterborne diseases. Most people affected are children under five years old. Symptoms of disease can include diarrhea, gastroenteritis, and paralysis. However, human infections can also be asymptomatic, which allows viruses to go undetected and circulate within the environment. Environmental surveillance is a tool that can supplement disease-based surveillance by sampling, concentrating, and analyzing sewage and other environmental surface waters for pathogens. Polioviruses and group A rotaviruses are enteric viruses of major global public health concern and water has been implicated in their transmission. Environmental surveillance for the detection of poliovirus has expanded in recent years to supplement clinical detection of wild polioviruses and vaccine-derived polioviruses in support of polio eradication efforts. Surveillance of other enteric viruses typically relies on disease-based surveillance and any environmental surveillance employed is for a limited sampling period. Recently, the introduction of new live, attenuated rotavirus vaccines has created a need for environmental monitoring to characterize rotavirus strains in wastewater. The epidemiology of these strains is useful to inform vaccine efficacy. This research focused on addressing environmental sampling needs. First, the evaluation and optimization of a secondary concentration step for improved detection of poliovirus in wastewater is presented. Next, environmental surveillance sampling is applied to enable molecular characterization of group A rotavirus strains in circulation in three communities in Nairobi, following the introduction of the Rotarix® vaccine in Kenya. A skimmed-milk flocculation (5%) method is identified as an economically feasible, time efficient, and high recovery secondary concentration method for poliovirus detection. Genetic characterization in selected Nairobi communities reveals a diversity of rotavirus strains in post-vaccine Kenya, with the emergence of serotype G3. Environmental surveillance for non-polio enteric viruses is often ad hoc to support outbreak investigations or inform vaccine efficacy. Though, it is expected to expand in coming years as better detection methods are developed and the global community pursues eradication of more vaccine preventable diseases.

Indicators for Waterborne Pathogens

Indicators for Waterborne Pathogens
Author: National Research Council
Publisher: National Academies Press
Total Pages: 328
Release: 2004-05-19
Genre: Nature
ISBN: 0309165962

Recent and forecasted advances in microbiology, molecular biology, and analytical chemistry have made it timely to reassess the current paradigm of relying predominantly or exclusively on traditional bacterial indicators for all types of waterborne pathogens. Nonetheless, indicator approaches will still be required for the foreseeable future because it is not practical or feasible to monitor for the complete spectrum of microorganisms that may occur in water, and many known pathogens are difficult to detect directly and reliably in water samples. This comprehensive report recommends the development and use of a "tool box" approach by the U.S Environmental Protection Agency and others for assessing microbial water quality in which available indicator organisms (and/or pathogens in some cases) and detection method(s) are matched to the requirements of a particular application. The report further recommends the use of a phased, three-level monitoring framework to support the selection of indicators and indicator approaches.Â

Methods For Recovering Viruses From The Environment

Methods For Recovering Viruses From The Environment
Author: Gerald Berg
Publisher: CRC Press
Total Pages: 242
Release: 2018-01-18
Genre: Science
ISBN: 1351082914

This book argues, that without methods, there can be no research. Effective research requires effective methods, not always easy to come by. The development of methods in environmental virology became a focus of growing interest about two decades ago. Progress has been significant since that time in pure experimental systems, where there are no interferences, consistent high recoveries of viruses from environmental waters has been achievable for some time. In the natural environment, however, in relatively clean waters, substances such as humic and fulvic acids interfere with viral recoveries and average recovery rates probably do not reach 20%. With sewage sludges and shellfish, recoveries are undoubtedly much lower. Yet, even relatively low viral recovery rates have made possible the detection of viral hazards in drinking waters. The hazards that exist are undoubtedly much greater than those demonstrated with the relatively inefficient methods inefficient methods developed thus far. Improving methods, as they are developed in the years to come, will undoubtedly bring the true extent of the hazards into better perspective.

Microbiology of Waterborne Diseases

Microbiology of Waterborne Diseases
Author:
Publisher: Academic Press
Total Pages: 719
Release: 2013-11-08
Genre: Science
ISBN: 0124159761

The second edition of Microbiology of Waterborne Diseases describes the diseases associated with water, their causative agents and the ways in which they gain access to water systems. The book is divided into sections covering bacteria, protozoa, and viruses. Other sections detail methods for detecting and identifying waterborne microorganisms, and the ways in which they are removed from water, including chlorine, ozone, and ultraviolet disinfection. The second edition of this handbook has been updated with information on biofilms and antimicrobial resistance. The impact of global warming and climate change phenomena on waterborne illnesses are also discussed. This book serves as an indispensable reference for public health microbiologists, water utility scientists, research water pollution microbiologists environmental health officers, consultants in communicable disease control and microbial water pollution students. Focuses on the microorganisms of most significance to public health, including E. coli, cryptosporidium, and enterovirus Highlights the basic microbiology, clinical features, survival in the environment, and gives a risk assessment for each pathogen Contains new material on antimicrobial resistance and biofilms Covers drinking water and both marine and freshwater recreational bathing waters

Comprehensive Water Quality and Purification

Comprehensive Water Quality and Purification
Author:
Publisher: Elsevier
Total Pages: 1537
Release: 2013-09-24
Genre: Technology & Engineering
ISBN: 0123821835

Comprehensive Water Quality and Purification, Four Volume Set provides a rich source of methods for analyzing water to assure its safety from natural and deliberate contaminants, including those that are added because of carelessness of human endeavors. Human development has great impact on water quality, and new contaminants are emerging every day. The issues of sampling for water analysis, regulatory considerations, and forensics in water quality and purity investigations are covered in detail. Microbial as well as chemical contaminations from inorganic compounds, radionuclides, volatile and semivolatile compounds, disinfectants, herbicides, and pharmaceuticals, including endocrine disruptors, are treated extensively. Researchers must be aware of all sources of contamination and know how to prescribe techniques for removing them from our water supply. Unlike other works published to date that concentrate on issues of water supply, water resource management, hydrology, and water use by industry, this work is more tightly focused on the monitoring and improvement of the quality of existing water supplies and the recovery of wastewater via new and standard separation techniques Using analytical chemistry methods, offers remediation advice on pollutants and contaminants in addition to providing the critical identification perspective The players in the global boom of water purification are numerous and varied. Having worked extensively in academia and industry, the Editor-in-Chief has been careful about constructing a work for a shared audience and cause