Enzymology in the Practice of Laboratory Medicine

Enzymology in the Practice of Laboratory Medicine
Author: Philip Blume
Publisher: Academic Press
Total Pages: 433
Release: 2014-05-10
Genre: Science
ISBN: 1483277704

Enzymology in the Practice of Laboratory Medicine focuses on disease processes, enzymatic reactions and activities, and diagnosis and management of diseases. The selection first offers information on developments in the search for mechanisms of enzymic catalysis; application of the spin labeling technique to biological membranes; and the properties of membrane bound enzymes involved in energy transduction processes. Discussions focus on possible clinical interrelationships of membrane function, mitochondrial membrane transactions, mechanical details of chymotryptic catalysis, and physical nature of globular proteins. The text then elaborates on metalloenzymes, enzyme measurement, and enzymology, clinical chemistry, and development of fast analyzers. The publication examines the analytical uses of immobilized enzymes; origin of serum enzymes and the basis for their variation; and the screening methods for glycolytic enzyme deficiencies in red blood cells. Topics include factors contributing to normal variation of enzyme activity, catabolism of enzymes, mechanism of release from cells to plasma, and determination of urea in blood and urine. The selection is a dependable reference for researchers interested in the application of enzymology in laboratory medicine.

Laboratory Guide to Enzymology

Laboratory Guide to Enzymology
Author: Geoffrey A. Holdgate
Publisher: John Wiley & Sons
Total Pages: 309
Release: 2024-04-09
Genre: Medical
ISBN: 1394179790

Understand how drug discovery incorporates the building blocks of complex life with this accessible guide Enzymes are among the fundamental building blocks of life, the proteins that catalyze some of the most important metabolic and biochemical reactions that sustain the complex organism. Understanding the unique structures and mechanisms of individual enzymes is key to medical treatment and pharmaceutical intervention, making it an especially essential aspect of drug discovery. The topic can appear challenging, however, as it requires command of interdisciplinary research combining biology, chemistry, and mathematics. Laboratory Guide to Enzymology brings these disciplines together to offer a practical, accessible overview of the lab-based work of creating effective enzyme essays. Beginning with a guide to the fundamentals of enzyme theory, the book connects that theory to a range of modern methods of computation and experimentation. The result is a detailed but highly readable volume elucidating the cornerstone of drug discovery research. Laboratory Guide to Enzymology readers will also find: Specific examples of enzyme application in drug discovery Detailed discussion of topics including enzyme catalysis, chemical kinetics, steady-state assays, and many more Appendices including a range of vital supplementary material Laboratory Guide to Enzymology is ideal for all biomedical and pharmaceutical researchers working in enzymology and assay development, as well as advanced students in the biomedical or biochemical sciences looking to develop a working knowledge of this field of research.

Laboratory Methods in Enzymology: Protein Part D

Laboratory Methods in Enzymology: Protein Part D
Author:
Publisher: Academic Press
Total Pages: 161
Release: 2015-06-18
Genre: Science
ISBN: 0128003359

The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 520 volumes and 40,000 chapters in the collection, much of the material is still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. In this volume, number 545, we have brought together a number of core protocols concentrating on protein, carefully written and edited by experts. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols Brings together a number of core protocols concentrating on protein

Laboratory Methods in Enzymology: Protein Part A

Laboratory Methods in Enzymology: Protein Part A
Author:
Publisher: Academic Press
Total Pages: 0
Release: 2014-01-31
Genre: Science
ISBN: 9780124200708

The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including: Pulse-chase analysis to measure protein degradation Labeling a protein with fluorophores using NHS ester derivitization Immunoaffinity purification of proteins Proteolytic affinity tag cleavage Purification of GST-tagged proteins

Diagnostic Enzymology

Diagnostic Enzymology
Author: Steven C. Kazmierczak
Publisher: de Gruyter
Total Pages: 0
Release: 2014
Genre: Clinical enzymology
ISBN: 9783110207248

This book is the2nd improved and expanded edition of "Clinical Enzymology" (Lott/Wolf, 1987). It includes case studies and guidelines for specialists of laboratory medicine and clinicians, devotes each chapter to a specific enzyme or protein marker, contains case studies and guidelines, a section on marker biochemistry and physiology as well as a section on special pathology and analysis. The clear, didactic structure and the multiple choice questions also make the book valuable reading for graduate students in the fields of clinical pathology and laboratory medicine.

Clinical Enzymology

Clinical Enzymology
Author: George Kaldor
Publisher: Praeger Publishers
Total Pages: 222
Release: 1983-04-01
Genre: Medical
ISBN: 9780275913991

Practical Enzymology

Practical Enzymology
Author: Hans Bisswanger
Publisher: John Wiley & Sons
Total Pages: 400
Release: 2019-06-26
Genre: Science
ISBN: 3527820817

A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.