Industrial biotechnology is the practice of using cells to generate industrially useful products. An enzyme is a protein that catalyzes, or speeds up, a chemical reaction. Enzymes are the focal point of biotechnological processes, without them biotechnology as a subject would not exist. The main advantage of enzymes compared to most other catalysts is their stereo, region and chemo selectivity and specificity. Enzymes are responsible for many essential biochemical reactions in micro organisms, plants, animals, and human beings. Biotechnology processes may have potential in energy production, specifically in the substitution of renewable plant biomass for fossil feedstock. This will depend on the development of enzymes able to degrade cellulose in plant biomass and designing methods to recycle or dispose of spent biomass. With time, research, and improved protein engineering methods, many enzymes have been genetically modified to be more effective at the desired temperatures, pH, or under other manufacturing conditions typically inhibitory to enzyme activity (e.g. harsh chemicals), making them more suitable and efficient for industrial or home applications. Enzymes are used in the extraction of natural products, as catalysts in organic chemistry, in clinical analysis, in industrial processes, and so on. The application of enzymes is found in many different fields and it is one of the good sectors to venture. In coming few years it is estimated that world enzyme demand will average annual increases of 6.3 percent. This book basically deals with principles of industrial enzymology, basis of utilization of soluble and immobilized, enzymes in industrial processes, principles of immobilization of enzymes, enzymes in clinical analysis principles, practical aspects of large-scale protein purification, the applications of enzymes in industry, use of enzymes in the extraction of natural products, data on techniques of enzyme immobilization and bio affinity procedures etc. In this book you can find all the basic information required on the fundamental aspects of the enzymes, their chemistry, bio chemistry as well as detailed information of their applications a wide variety of industrial processes etc. The book is very useful for research scholars, technocrats, institutional libraries and entrepreneurs who want to enter into the field of manufacturing of enzymes. 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