Enzyme Histochemistry

Enzyme Histochemistry
Author: Z. Lojda
Publisher: Springer Science & Business Media
Total Pages: 350
Release: 2012-12-06
Genre: Medical
ISBN: 3642672345

During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.

Histochemistry

Histochemistry
Author: Jinsong Zhou
Publisher: Walter de Gruyter GmbH & Co KG
Total Pages: 244
Release: 2017-08-21
Genre: Science
ISBN: 3110531399

This book systematically illustrates theories and technologies in Histochemistry, including different kinds of enzymes, immunohistochemistry, polymerase chain reaction, related electron microscopic cytochemical techniques as well as the quantitative assay metrology. Abundant experiments as well as vivid images are demonstrated, making the book an essential reference for both graduate students and researchers in biochemistry.

Enzyme Histochemistry

Enzyme Histochemistry
Author: John D. Bancroft
Publisher: Oxford University Press, USA
Total Pages: 84
Release: 1987
Genre: Language Arts & Disciplines
ISBN:

Enzyme histochemical methods depend on visual identification of reaction products for the identification and localization of specific enzymes within tissue. This concise handbook is designed to give students, pathology, MLSO's and research workers an outline and handy lab reference to the methods which have become routine in research and hospital histopathological laboratories. The author discusses the most reliable and diagnostically important methods, and offers suggestions for further reading.

Histological & Histochemical Methods

Histological & Histochemical Methods
Author: John Alan Kiernan
Publisher: Pergamon
Total Pages: 454
Release: 1990
Genre: Medical
ISBN:

Revised and updated edition (1st was 1981) of a textbook on chemical and physical principles of fixation, staining and histochemistry. For students i all biological subjects using histological techniques, as well as researcher and medical laboratory technologists. Annotation copyright Book News, Inc

Histochemistry of Single Molecules

Histochemistry of Single Molecules
Author: Carlo Pellicciari
Publisher: Springer Nature
Total Pages: 351
Release: 2022-09-24
Genre: Science
ISBN: 1071626752

This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.

Trends in Enzyme Histochemistry and Cytochemistry

Trends in Enzyme Histochemistry and Cytochemistry
Author: David Evered
Publisher: John Wiley & Sons
Total Pages: 192
Release: 2009-09-16
Genre: Science
ISBN: 0470718242

The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.

Histochemical Techniques

Histochemical Techniques
Author: J. D. Bancroft
Publisher: Butterworth-Heinemann
Total Pages: 363
Release: 2013-10-22
Genre: Science
ISBN: 1483164713

Histochemical Techniques, 2nd Edition deals with different methods of tissue processing and their application to histochemical procedures, including freeze drying and freeze substitution. Topics covered include fixation, production of paraffin and frozen sections, and principles of enzyme histochemistry. The use of histochemical methods to demonstrate carbohydrates, proteins, amino acids, lipids, nucleic acids, pigments, and various enzymes is also discussed. This book is comprised of 20 chapters and begins with an overview of the problems and values of histochemistry. The discussion then turns to carbohydrates, along with concepts and methods in carbohydrate histochemistry such as blocking and enzyme methods. Other chapters focus on amyloids, pigments, minerals, phosphatases, and esterases. The principles of enzyme histochemistry are also considered, with emphasis on preservation and factors affecting enzyme activity. The final chapter is devoted to ultra-histochemistry, the application of histochemistry to electron microscopy. This monograph will be of interest to histochemists, histopathologists, and technologists involved in histochemical work.

Development of the Rat Spinal Cord: Immuno- and Enzyme Histochemical Approaches

Development of the Rat Spinal Cord: Immuno- and Enzyme Histochemical Approaches
Author: Martin F. Bach
Publisher: Springer Science & Business Media
Total Pages: 263
Release: 2012-12-06
Genre: Medical
ISBN: 3642784747

The studies described here were carried out in the Neuroregul ation Group, Department of Physiology, University of Leiden, the Netherlands. Over the last decade, this group, in close collaboration with the Department of Neurosurgery of the Academic Hospital of Leiden, has studied the development of the central nervous system from a neuroanatomical as well as a clinical perspective. During this period, the expression of several morphore gulators in the developing rat spinal cord was extensively investigated. Parallel studies focused on the development of the spinal cord fiber systems, which was studied by means of the intrauterine use of neuronal tracers. The main goal of these studies was to extend our knowledge about the (normal) generation of the spinal cord and to contribute to the under standing of clinical problems related to regeneration and degeneration in the mammalian central nervous system. The studies on morphoregulators, in particular, appeared to benefit two different scientific areas. Firstly, the correlation between morphoregulator expression patterns and known anatomy contributed to our knowledge about spinal cord development. Secondly, the correlation between morpho regulator expression patterns and known developmental processes may help to understand their precise function(s). This volume of Advances in Anatomy, Embryology and Cell Biology presents these particular studies on the development of the rat spinal cord performed over the last decade. As well as integrating the results of the tracer studies, this volume also provides an update on the development of the rat spinal cord.

Histochemical and Cytochemical Methods of Visualization

Histochemical and Cytochemical Methods of Visualization
Author: Jean-Marie Exbrayat
Publisher: CRC Press
Total Pages: 339
Release: 2013-06-05
Genre: Science
ISBN: 1439822255

Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualizatio

Methods in Plant Cell Biology

Methods in Plant Cell Biology
Author: David W. Galbraith
Publisher: Academic Press
Total Pages: 605
Release: 1995
Genre: Medical
ISBN: 0125641516

Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants.