Energetics Of Biological Macromolecules Part E
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| Author | : |
| Publisher | : Elsevier |
| Total Pages | : 479 |
| Release | : 2004-04-02 |
| Genre | : Science |
| ISBN | : 0080497187 |
Energetics of Biological Macromolecules, Part E focuses on methods related to allosteric enzymes and receptors, including fluorescent proves, spectroscopic methods and quantitative analysis as well as on cooperativity in protein folding. NMR and mass spectrometry methods are discussed. - Allosteric Enzymes and Receptors - Cooperativity in Protein Folding and Assembly
| Author | : Jo M. Holt |
| Publisher | : Elsevier |
| Total Pages | : 315 |
| Release | : 2004-04-02 |
| Genre | : Science |
| ISBN | : 0080497179 |
This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed.*Methods for Evaluating Cooperativity in a Dimeric Hemoglobin*Multiple-Binding of Ligands to a Linear Biopolymer*Fluorescence Quenching Methods to Study Protein-Nucleic Acid Interactions*Linked Equilibria in Biotin Repressor Function: Thermodynamic, Structural and Kinetic Analysis
| Author | : |
| Publisher | : Elsevier |
| Total Pages | : 581 |
| Release | : 2000-08-09 |
| Genre | : Science |
| ISBN | : 0080496784 |
Volume 323 of Methods in Enzymology is dedicated to the energetics of biological macromolecules. Understanding the molecular mechanisms underlying a biological process requires detailed knowledge of the structural relationships within the system and an equally detailed understanding of the energetic driving forces that control the structural interactions. This volume presents modern thermodynamic techniques currently being utilized to study the energetic driving forces in biological systems. It will be a useful reference source and textbook for scientists and students whose goal is to understand the energetic relationships between macromoleculer structures and biological functions. This volume supplements Volumes 259 and Volume 295 of Methods in Enzymology.Key Features* Probing Stability of Helical Transmembrane Proteins* Energetics of Vinca Alkaloid Interactions with Tubulin* Deriving Complex Ligand Binding Formulas* Mathematical Modeling of Cooperative Interactions in Hemoglobin* Analysis of Interactions of Regulatory Protein TyrR with DNA* Parsing Free Energy of Drug-DNA Interactions* Use of Fluorescence as Thermodynamics Tool
| Author | : |
| Publisher | : Academic Press |
| Total Pages | : 395 |
| Release | : 2013-07-11 |
| Genre | : Science |
| ISBN | : 0124055400 |
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers hydrogen peroxide and cell signaling - Contains chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy
| Author | : Helmut Sies |
| Publisher | : Elsevier |
| Total Pages | : 461 |
| Release | : 2004-05-01 |
| Genre | : Science |
| ISBN | : 0080497160 |
Quinones are members of a class of aromatic compounds with two oxygen atoms bonded to the ring as carbonyl groups. This volume covers the role of quinines enzymes in cellular signalling and modulation of gene expression.*Coenzyme Q: Detection and Quinone Reductases*Plasma Membrane Quinone Reductases*Quinones, Cellular Signaling, and Modulation of Gene Expression
| Author | : |
| Publisher | : Academic Press |
| Total Pages | : 463 |
| Release | : 2012-10-01 |
| Genre | : Science |
| ISBN | : 012394628X |
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The second of 3 volumes covering Natural product biosynthesis by microorganisms and plants. - This new volume continues the legacy of this premier serial - Contains quality chapters authored by leaders in the field - The second of 3 volumes it has chapters on such topics as biological chlorination, bromination and iodination, and phylogenetic approaches to natural product structure prediction
| Author | : Christopher A. Voigt |
| Publisher | : Academic Press |
| Total Pages | : 557 |
| Release | : 2011 |
| Genre | : Bioengineering |
| ISBN | : 0123851203 |
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines and many different definitions exist. This volume covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation.
| Author | : Carl Wu |
| Publisher | : Elsevier |
| Total Pages | : 579 |
| Release | : 2004-02-20 |
| Genre | : Science |
| ISBN | : 0080497136 |
DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodellng enzymes play an important role in gene transcription.*Histone Bioinformatics*Biochemistry of histones, nucleosomes and chromatin*Molecular cytology of chromatin functions
| Author | : Carl Wu |
| Publisher | : Elsevier |
| Total Pages | : 585 |
| Release | : 2004-04-07 |
| Genre | : Science |
| ISBN | : 0080497152 |
DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the chromatin remodelling enzymes play an important role in gene transcription. - Genetic assays of chromatin modification and remodeling - Histone modifying enzymes - ATP-dependent chromatin remodeling enzymes
| Author | : |
| Publisher | : Elsevier |
| Total Pages | : 292 |
| Release | : 2007-11-09 |
| Genre | : Science |
| ISBN | : 0080560652 |
This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Liquid chromatography mass spectrometry for quantifying plasma lysophospholipids: potential biomarkers for cancer diagnosis; Measurement of eicosanoids in cancer tissues; Noninvasive Assessment of the Role of Cyclooxygenases in Cardiovascular HealthA Detailed HPLC/MS/MS Method; Lipidomics in Diabetes and the Metabolic Syndrome; LC-MS-MS Analysis of Neutral Eicosanoids; Quantification Of F2-Isoprostanes In Biological Fluids And Tissues As A Measure Of Oxidant Stress; Measurement of Products of Docosahexaenoic Acid Peroxidation, Neuroprostanes, and Neurofurans; Enantiomeric separation of hydroxy and hydroperoxy eicosanoids by chiral column chromatography; Targeted Chiral Lipidomics Analysis by Liquid Chromatography Electron Capture Atmospheric Pressure Chemical Ionization Mass Spectrometry (LC-ECAPCI/MS); Shotgun Lipidomics by Tandem Mass Spectrometry under Data-Dependent Acquisition Control; Identification of Intact Lipid Peroxides by Ag+ Coordination Ionspray Mass Spectrometry (CIS-MS); Quantification of Cardiolipin by Liquid Chromatography Electrospray Ionization Mass Spectrometry.
