Electron Microscopy Of Plant Pathogens
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Author | : Kurt Mendgen |
Publisher | : Springer Science & Business Media |
Total Pages | : 342 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 3642758185 |
Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Author | : Aiming Wang |
Publisher | : Humana |
Total Pages | : 0 |
Release | : 2022-12-29 |
Genre | : Science |
ISBN | : 9781071618370 |
This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.
Author | : R. E. F. Matthews |
Publisher | : CRC Press |
Total Pages | : 392 |
Release | : 2019-07-23 |
Genre | : Science |
ISBN | : 1351079808 |
Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.
Author | : R. E. F. Matthews |
Publisher | : CRC Press |
Total Pages | : 400 |
Release | : 1993-08-03 |
Genre | : Science |
ISBN | : |
Overview; Host plants in diagnosis; Experimental transmission of viruses in diagnosis; Virus transmission through soil and by soil-inhabiting; Organisms in diagnosis; Inclusions in diagnosing plant virus diseases; Virus purification in relation to diagnosis; Serological procedures; Electron microscopy of in vitro preparations; Nucleic acid hybridization procedures; dsRNA in diagnosis; Diagnostic methods applicable no viroids; Strategies for implicating virus-like pathogens as the caused of diseases of unknown etiology; Complexes of transmission-dependent and helper viruses.
Author | : Chelston W. D. Brathwaite |
Publisher | : IICA |
Total Pages | : 56 |
Release | : 1981 |
Genre | : Plant deseases |
ISBN | : 9789290390138 |
The diagnosis of plant disease; The microscope; The autoclave; The preparation of media for fungal and bacterial growth; Detection of fungal pathogens in infected plant tissues; Detection of bacterial pathogens in infected tissues; Koch's postulates; The diagnosis of a nematode prblem; Viruses and plant virus diseases; Mycoplasma as agents of plant disease.
Author | : P. Narayanasamy |
Publisher | : CRC Press |
Total Pages | : 541 |
Release | : 2001-08-28 |
Genre | : Science |
ISBN | : 1482270951 |
This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materia
Author | : Kurt Mendgen |
Publisher | : Springer |
Total Pages | : 336 |
Release | : 1990-12-10 |
Genre | : Science |
ISBN | : 9783540527770 |
Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Author | : Melvin Dennis Bolton |
Publisher | : Springer Science+Business Media |
Total Pages | : 648 |
Release | : 2012 |
Genre | : Science |
ISBN | : 9781617795015 |
Including reproducible laboratory protocols, this guide to fungal pathogens in plants has been written by expert researchers in the field and includes methods now used to study them, including techniques for model systems such as Arabidopsis thaliana.
Author | : Gary Phillip Munkvold |
Publisher | : |
Total Pages | : 165 |
Release | : 2016 |
Genre | : Corn |
ISBN | : 9780890544921 |
Author | : Milos Janecek |
Publisher | : BoD – Books on Demand |
Total Pages | : 302 |
Release | : 2016-02-18 |
Genre | : Science |
ISBN | : 9535122525 |
This book brings a broad review of recent global developments in theory, instrumentation, and practical applications of electron microscopy. It was created by 13 contributions from experts in different fields of electron microscopy and technology from over 20 research institutes worldwide.