Electron Microscopy In Molecular Biology
Download Electron Microscopy In Molecular Biology full books in PDF, epub, and Kindle. Read online free Electron Microscopy In Molecular Biology ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Author | : Michael J. Dykstra |
Publisher | : Springer Science & Business Media |
Total Pages | : 368 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 146840010X |
In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.
Author | : John Kuo |
Publisher | : Springer Science & Business Media |
Total Pages | : 606 |
Release | : 2008-02-05 |
Genre | : Science |
ISBN | : 1597452947 |
This book presents the newest technology in electron microscopy. It comprises two major areas of electron microscopy - transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. A notes section enables efficient adaptation and troubleshooting of protocols.
Author | : Nicolas Rasmussen |
Publisher | : Stanford University Press |
Total Pages | : 426 |
Release | : 1999-07-01 |
Genre | : Science |
ISBN | : 9780804738507 |
This first detailed historical treatment of the electron microscope in biology advances an original philosophical argument on the relation of experimental technology to scientific change.
Author | : M. A. Nasser Hajibagheri |
Publisher | : Springer Science & Business Media |
Total Pages | : 292 |
Release | : 2008-02-02 |
Genre | : Science |
ISBN | : 1592592015 |
Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).
Author | : Heide Schatten |
Publisher | : Cambridge University Press |
Total Pages | : 275 |
Release | : 2013 |
Genre | : Science |
ISBN | : 0521195993 |
A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.
Author | : Douglas E. Chandler |
Publisher | : Jones & Bartlett Publishers |
Total Pages | : 468 |
Release | : 2009 |
Genre | : Medical |
ISBN | : 0763738743 |
The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.
Author | : Michael J. Dykstra |
Publisher | : Springer Science & Business Media |
Total Pages | : 558 |
Release | : 2003-12-31 |
Genre | : Science |
ISBN | : 9780306477492 |
Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.
Author | : G. H. Haggis |
Publisher | : |
Total Pages | : 96 |
Release | : 1967 |
Genre | : Medical |
ISBN | : |
Author | : |
Publisher | : Academic Press |
Total Pages | : 562 |
Release | : 2008-10-22 |
Genre | : Science |
ISBN | : 008088816X |
This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). Basic techniques in transmission and scanning electron microscopy Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton Discussion on electron microscopy of viruses and virus-cell interactions
Author | : John Sommerville |
Publisher | : |
Total Pages | : 272 |
Release | : 1987 |
Genre | : Science |
ISBN | : |
Vast experience has gone into providing this comprehensive guide to analysis at the ultrastructural level of nucleic acids, proteins and macromolecular complexes. Research workers will find it a unique handbook, containing procedures for applications with a wide range of biologically important macromolecules.