Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications

Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications
Author: Erika Pestana
Publisher: Springer Science & Business Media
Total Pages: 320
Release: 2010-01-18
Genre: Science
ISBN: 9048131324

This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. The book is an adjunct to the Molecular Diagnostic PCR Handbook published in 2005.

Evolving Landscape of Molecular Diagnostics

Evolving Landscape of Molecular Diagnostics
Author: Mrutyunjay Suar
Publisher: Elsevier
Total Pages: 276
Release: 2024-06-27
Genre: Science
ISBN: 0323993176

Evolving Landscape of Molecular Diagnostics: Applications and Techniques presents cutting-edge reviews on current and emerging technologies in the diagnosis of microbial infections. The book discusses the fundamentals of Molecular Diagnostics for bacteria, fungi, viruses and a variety of state-of-the-art diagnostics tools and techniques for obtaining qualitative and quantitative results. It includes topics such as Next generation sequencing and application of "OMICS" for early disease diagnosis. Sections cover the entire spectrum of emerging diagnostic tools and techniques, as well as the principles, pros and cons of each method and applications for diagnosis of infectious disease in plants, humans and veterinary. This is a resource for both researchers and students working in clinical microbiology, infectious biology, applied life sciences and scientists working in the clinical diagnostic industry. - Provides a wide range of diagnostics tools and technologies in the field of infectious biology - Presents discussions on emerging technologies for early disease diagnosis and novel diagnostics techniques for detection of COVID, Urea Breath Tests for Detection of Helicobacter pylori, Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis, Molecular imaging, Lab-on-a-chip Technologies, and several others - Analyzes literature reviews, underpinning methodologies, and opportunities and limitations - Includes case studies and examples that highlight applications using the underpinning techniques

Gene Quantification

Gene Quantification
Author: Francois Ferre
Publisher: Springer Science & Business Media
Total Pages: 379
Release: 2012-12-06
Genre: Medical
ISBN: 1461241642

Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.

Greene's Infectious Diseases of the Dog and Cat - E-Book

Greene's Infectious Diseases of the Dog and Cat - E-Book
Author: Jane E. Sykes
Publisher: Elsevier Health Sciences
Total Pages: 1828
Release: 2022-06-24
Genre: Medical
ISBN: 0323509339

**Selected for Doody's Core Titles® 2024 with "Essential Purchase" designation in Veterinary Medicine** Greene's Infectious Diseases of the Dog and Cat, 5th Edition provides a comprehensive, clinically useful reference on the management of infectious diseases caused by viruses, bacteria (including rickettsiae, chlamydiae, mycoplasmas, and spirochetes), fungi, algae, protozoa, parasites, and other atypical agents. Each section guides the reader through diagnostic testing for specific infectious diseases, from specimen collection to laboratory submission to interpretation of results to appropriate treatment measures. Full-color illustrations and hundreds of tables provide convenient access to diagnostic and therapeutic recommendations, along with the appropriate drug dosages for effective treatment and prevention. A fully searchable enhanced eBook version is included with print purchase, allowing access to all of the text and figures on a variety of digital devices. - More than 150 internationally recognized experts contribute chapters on topics in their field of specialty. - Clear and logical organization of chapters provides a solid basis for an approach to diseases caused by specific pathogens, with the first part of the book including sections on diagnostic approaches, treatments (including recommended antimicrobial drug doses), and prevention. - Specific pathogens are addressed in the second part of the book, using a structured approach that includes etiology/epidemiology (relevance to wildlife animal hosts, role of the environment), clinical and laboratory findings, treatment, prevention, and public health implications. - Case examples illustrate principles and highlight how the material can be applied. - More than 800 clinical images, maps, life cycles, and photomicrographs assist with accurate understanding of epidemiology, pathogenesis, diagnosis of disease, and disease prevention. - Visually appealing maps and life-cycle drawings enhance your comprehension and retention of the material. - Convenient drug dosage tables in each chapter provide complete prescribing information; chapters on antimicrobial drugs in the first part of the book summarize pharmacokinetics, indications, contraindications, handling and administration guidelines; and dosage recommendations are made for antivirals, antibacterials, antifungals, antiprotozoals, and antiparasitic drugs. The book emphasizes approaches to optimize antimicrobial stewardship. - Clinical Problems section helps you understand what infectious diseases should be considered in animals seen with clinical signs relating to different organ systems. - Suggested readings and references are listed in each chapter, facilitating further research and study. - Fully searchable enhanced eBook version is included with print purchase, allowing access to all of the text and figures on a variety of digital devices.

Diagnostic Procedures in Ophthalmology

Diagnostic Procedures in Ophthalmology
Author: HV Nema
Publisher: JP Medical Ltd
Total Pages: 484
Release: 2014-05-30
Genre: Medical
ISBN: 9350908522

New edition presenting latest developments in ophthalmic diagnostic procedures. Fully revised and many new chapters. Previous edition published in 2009.

Peste des Petits Ruminants Virus

Peste des Petits Ruminants Virus
Author: Muhammad Munir
Publisher: Springer
Total Pages: 261
Release: 2014-12-02
Genre: Medical
ISBN: 3662451654

This book offers a timely and comprehensive review of essential research on Peste des Petits Ruminants Virus (PPRV), ranging from its historical distribution, molecular epidemiology, genome structure, viral proteins, immunity, viral pathogenesis, clinical and molecular diagnosis to advances in vaccine developments and future challenges. PPRV, a Rinderpest-like virus, is the causative agent of one of the most rapidly emerging viral diseases among domestic small ruminants, and the host spectrum has now been expanded to wild small ruminants and camels. With the global eradication of the first livestock disease, Rinderpest, attention is now turning to repeating the procedure for PPR. Each of the book’s 13 chapters is dedicated to a specific topic, providing up-to-date literature and discussions by renowned scientists who have made seminal contributions in their respective fields of expertise. Special emphasis has been placed on the analysis of different global efforts to eradicate PPR. This book offers a valuable reference source for virologists, field veterinarians, infection and molecular biologists, immunologists, scientists in related fields and veterinary school libraries.

Genomics and Biotechnological Advances in Veterinary, Poultry, and Fisheries

Genomics and Biotechnological Advances in Veterinary, Poultry, and Fisheries
Author: Yashpal Singh Malik
Publisher: Academic Press
Total Pages: 565
Release: 2019-09-14
Genre: Technology & Engineering
ISBN: 0128163534

Genomics and Biotechnological Advances in Veterinary, Poultry, and Fisheries is a comprehensive reference for animal biotechnologists, veterinary clinicians, fishery scientists, and anyone who needs to understand the latest advances in the field of next generation sequencing and genomic editing in animals and fish. This essential reference provides information on genomics and the advanced technologies used to enhance the production and management of farm and pet animals, commercial and non-commercial birds, and aquatic animals used for food and research purposes. This resource will help the animal biotechnology research community understand the latest knowledge and trends in this field. - Presents biological applications of cattle, poultry, marine and animal pathogen genomics - Discusses the relevance of biomarkers to improve farm animals and fishery - Includes recent approaches in cloning and transgenic cattle, poultry and fish production

Clinical Veterinary Microbiology E-Book

Clinical Veterinary Microbiology E-Book
Author: Bryan Markey
Publisher: Elsevier Health Sciences
Total Pages: 915
Release: 2013-11-30
Genre: Medical
ISBN: 0702055883

This beautifully illustrated, comprehensive reference provides concise information on the materials and methods of bacteriology, mycology, and virology. The book covers the collection, isolation, and culture of diagnostic specimens, with detailed notes on the biochemical, serological and other tests currently used to identify and distinguish between microbial pathogens. The new edition sets out to provide the most up-to-date account of all the clinically and economically important pathogens, including Bovine Spongiform Encephalomyeltis, Creutzfeldt-Jakob Disease, E-coli, and Salmonella. The clear, accessible format, together with the complete revision of the content, makes this a valuable resource. - High quality full colour photography - Essential for accurate diagnosis - Fully revised pathogenicity sections taking into account the major discoveries/incidences of the last 3-5 years - Reclassification of viruses, including changes to nomenclature - Appendices of Infectious Diseases - Fast access to vital information - Unique and practical inclusion of virology, bacteriology and mycology in one text - Greatly expanded chapter on viruses - More on PRIONS (including BSE) - Reclassification of viruses - many changes to nomenclature - Fully revised pathogenicity sections - Revised/complete coverage of E coli 0157 - Revised Systems section - Complete update of Infectious Diseases coverage in the appendices

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies
Author: Mónica V. Cunha
Publisher: Humana Press
Total Pages: 0
Release: 2014-11-16
Genre: Medical
ISBN: 9781493920037

This volume provides an overview of molecular biology tools for pathogen detection and characterization. Focusing on biobanking, biosafety, good laboratory practices, biological specimen collection and processing, quality assurance and control, validation of molecular diagnostic assays for veterinary use; molecular detection and identification of animal pathogens using a wide range of established techniques and covering, also, emerging diagnosis approaches; genotyping tools for assessing the genetic landscape and epidemiology of pathogens; and integrative omics and high-throughput technologies as powerful research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies aids scientists in continuing to study a broad range of molecular approaches, from simple and affordable to emerging and sophisticate.