E is for Elisa

E is for Elisa
Author: Johanna Hurwitz
Publisher: StarWalk Kids Media
Total Pages: 115
Release: 2012-10-01
Genre: Juvenile Fiction
ISBN: 1623342104

"Crybaby, Crybaby!" That's what Russell calls Elisa whenever she gets upset, and she hates it. Elisa does get frustrated sometimes, like when she can't wear her favorite red shirt to school. But that doesn't mean she can't do other things -- like trade her mittens for a tooth so that the tooth fairy will come sooner, or have a beach party in the middle of winter -- whenever and however she wants!"

Our Life Beyond Mkultra

Our Life Beyond Mkultra
Author: Elisa E
Publisher: CreateSpace
Total Pages: 222
Release: 2013-08
Genre: Biography & Autobiography
ISBN: 9781490972855

Our Life Beyond MKULTRA, Books 1 and 2, is an insider's story of intentionally designed trauma-based mind kontrol Multiple Personality Disorder and her resultant use involving government, military, intelligence, religious and corporate organizations and institutions, as well as the insidious kontrol of humanity by a eons old cross-dimensional species involved in hybridization and cloning. The demi-gods and Nephilim of old have a predominant role in her story, having encountered them repeatedly in the altered consciousness of trauma-based mind kontrol programming as well as in full waking consciousness. It is a journey through the mind fragmenting "black tech" being utilized by a bloodline network of human victims to these malevolent beings who are now its minions and perpetrators. Elisa's is a story of abilities attained through this process some of which still remain well into her deep deprogramming years which began in April 2008. Her story takes you through hybridization, cloning and cybernization, though not in the future, instead already here and successfully implemented in the black projects of high level total mind kontrol. Elisa contends she is a hybrid, preconception, and in the late 1980s became aware of her clones. What's more, she speaks to the current state of mind kontrol technology in use as involving not only hybridization and cloning, but post-birth cybernization and the design of human looking beings who are actually soulless, and that some already walk among us. Our Life Beyond MKULTRA is contained in 2 Books to provide the perspective of not only the horrendous programming and "on task" years running decades, but the life threatening journey that continues in deep deprogramming. Her contention, "once MKULTRA, always MKULTRA," however the dismantling and manageability of her still surfacing alters and programs has allowed an intelligent and self-aware evaluation of not only her own situation, yet that of the "next level" End Time agenda now affecting the globe. If the current intellect continues to demand that all that is surreal is a judge to falsehoods, then humanity will not extract itself from the impending species kontrol. According to Elisa and many of her alternate personalities, the "next level" is the total kontrol of the human species without their recognition that it occurred.

The ELISA Guidebook

The ELISA Guidebook
Author: John R. Crowther
Publisher: Humana
Total Pages: 0
Release: 2010-11-19
Genre: Medical
ISBN: 9781617378843

There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.

ELISA

ELISA
Author: John R. Crowther
Publisher: Springer Science & Business Media
Total Pages: 231
Release: 2008-02-02
Genre: Medical
ISBN: 1592595294

ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.

ELISA

ELISA
Author: Jose Ma. M. Angeles
Publisher: Nova Science Publishers
Total Pages: 254
Release: 2022
Genre: Medical
ISBN: 9781685075941

"In September 1971, Swiss scientists Eva Engvall and Peter Perlmann published a communication in the journal Immunochemistry on enzyme-linked immunosorbent assay (ELISA) as a quantitative assay of immunoglobulin G. They have modified radioimmunoassay (RIA) by replacing the radioactive iodine with enzymes and used in determining the levels of IgG in rabbit serum. Fifty years after, ELISA is still the most commonly used technique in measuring peptides, proteins, antibodies, antigens, and hormones in various samples. This plate-based assay is a very versatile laboratory technique and offers many advantages to laboratory scientists and researchers such as generating quick results, simple protocols, high sensitivity, being easily automated and being convenient to perform. Its application in the diagnosis of different infectious diseases has shown the unquestionable importance of this technique even in the modern world. In this book, the usefulness of ELISA in the control of different parasitic diseases including schistosomiasis, babesiosis, and malaria will be discussed. There is also a chapter featuring an extensive discussion on the use of ELISA in functional food development and regulation. And finally, the last two chapters will present advanced techniques inspired by ELISA including a rapid immunoassay for detecting paralytic shellfish poisoning toxins and the enzyme-linked aptamer assay. May this book provide significant information regarding the current advances and applications of ELISA and possibly inspire its use in another 50 years"--

Enzyme-linked Immunosorbent Assay (ELISA)

Enzyme-linked Immunosorbent Assay (ELISA)
Author: Samira Hosseini
Publisher: Springer
Total Pages: 124
Release: 2017-12-30
Genre: Technology & Engineering
ISBN: 981106766X

This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.

Elisa Notebook

Elisa Notebook
Author: Customeyes PUBLICATIONS
Publisher: Independently Published
Total Pages: 104
Release: 2019-04-17
Genre:
ISBN: 9781095031612

***** CLICK THE AUTHOR NAME "CUSTOMEYES PUBLICATIONS" FOR MORE PLANNERS, NOTEBOOKS & DIARIES ***** Be prepared and keep yourself organized for anything with this stylish Notebook! The perfect companion to write about your life experiences. This name customized Notebook provides the ideal way to stay organized. A special place to record daily events, record small wins, arm yourself with words of wisdom and capturing brilliant ideas. It's also a popular tool for documenting your daily life. This matte finished Notebook comes complete with over 100 pages (approx. 52 sheets). It has a flexible lightweight paperback cover, which makes it lighter and easier to carry around, and comes complete with a cool & trendy cover. Dimensions: 8.5" x 11" giving plenty of writing space to prepare for each day ahead. This Notebook is perfect to help: Keep on top of tasks & activities Stay organized with planning Keep track of personal health & medications Noting down things you want to do or read Documenting Life Noting down ideas for blog writing or other forms of writing And so much more... Time to take the stress out of your life and become more organized. Set yourself up for success to help you reach your goals and aspirations with this cute Notebook. Order yours now!