Dna Mediated Assembly Of Nanostructured Materials
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| Author | : So-Jung Park |
| Publisher | : |
| Total Pages | : |
| Release | : 2002 |
| Genre | : |
| ISBN | : |
Based on this observation, a DNA array detection method was developed in which the binding of Au nanoparticles functionalized with oligonucleotides led to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. Using this method, we have detected target DNA at concentrations as low as 500 fM with a point mutation selectivity factor of ∼100,000:1. These unprecedented capabilities point towards a way of eliminating the need for polymerase chain reaction (PCR) in DNA detection.
| Author | : Jennifer J. McManus |
| Publisher | : Humana |
| Total Pages | : 266 |
| Release | : 2020-08-08 |
| Genre | : Science |
| ISBN | : 9781493996803 |
This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
| Author | : Chunhai Fan |
| Publisher | : Springer Nature |
| Total Pages | : 406 |
| Release | : 2020-09-07 |
| Genre | : Technology & Engineering |
| ISBN | : 3030548066 |
The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field. The chapter "DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials" is available open access under a CC BY 4.0 License via link.springer.com.
| Author | : Björn Högberg |
| Publisher | : |
| Total Pages | : 74 |
| Release | : 2007 |
| Genre | : |
| ISBN | : 9789185317394 |
| Author | : Nadrian C. Seeman |
| Publisher | : Cambridge University Press |
| Total Pages | : 269 |
| Release | : 2015 |
| Genre | : Computers |
| ISBN | : 0521764483 |
Written by the founder of the field, this is a comprehensive and accessible introduction to structural DNA nanotechnology.
| Author | : Phyllis F. Xu |
| Publisher | : |
| Total Pages | : 97 |
| Release | : 2013 |
| Genre | : |
| ISBN | : 9781303379796 |
Traditionally known as the genetic code, in recent years DNA has been engineered in new ways and applied toward novel applications in materials science and bio-nanotechnology. The nanometer features and highly specific base-pairing of DNA has enabled its use to build complex 2- and 3D nano-architectures and nano-structures. Specific DNA scaffolds include branched DNA junctions, 2D and 3D DNA "tiles" and "bricks", and DNA hydrogels that can be used for applications across various fields of materials science. Another unique feature of DNA is its ability to bind other materials through molecular recognition which has made the use of DNA aptamer or DNA-protein conjugates highly useful for applications in biosensing and identification. The main focus of this thesis is to demonstrate the use of DNA as an invaluable tool for engineering specific nanostructures for biosensing. The first chapter introduces the basics of DNA and current research in various DNA structures and assemblies and their applications. The second and third chapters describe the self-assembly of two structurally different nanomaterials using DNA, carbon nanotubes and gold nanoparticles. In the second chapter, the successful large-scale alignment of carbon nanotubes on a surface is shown. The study goes into the factors that affect the quality of alignment, including salt concentration, length of DNA, and annealing. In the third chapter, we show how DNA can be used to engineer discrete gold nanocrystal assemblies-"nanodumbbells"- that can adopt different structural conformations through DNA interactions. By incorporating DNA aptamers in the nanoparticle structure, these nanodumbbells can be used to sense particular analytes in solution. Chapter 4 continues the study of the nanodumbbell structures and their potential use as surface-enhanced Raman spectroscopy (SERS) biosensors. A large measurable difference in Raman signal was experimentally obtained from the two distinct nanodumbbell conformations. This was compared with theoretical studies of SERS enhancement to validate the signals produced. We were also able to show the successful detection of an analyte, ATP, using the DNA-mediated probe and SERS detection method.
| Author | : Ampere A. Tseng |
| Publisher | : World Scientific |
| Total Pages | : 583 |
| Release | : 2008 |
| Genre | : Technology & Engineering |
| ISBN | : 9812790896 |
Many of the devices and systems used in modern industry are becoming progressively smaller and have reached the nanoscale domain. Nanofabrication aims at building nanoscale structures, which can act as components, devices, or systems, in large quantities at potentially low cost. Nanofabrication is vital to all nanotechnology fields, especially for the realization of nanotechnology that involves the traditional areas across engineering and science. This is the first book solely dedicated to the manufacturing technology in nanoscale structures, devices, and systems and is designed to satisfy the growing demands of researchers, professionals, and graduate students. Both conventional and non-conventional fabrication technologies are introduced with emphasis on multidisciplinary principles, methodologies, and practical applications. While conventional technologies consider the emerging techniques developed for next generation lithography, non-conventional techniques include scanning probe microscopy lithography, self-assembly, and imprint lithography, as well as techniques specifically developed for making carbon tubes and molecular circuits and devices. Sample Chapter(s). Chapter 1: Atom, Molecule, and Nanocluster Manipulations for Nanostructure Fabrication Using Scanning Probe Microscopy (3,320 KB). Contents: Atomic Force Microscope Lithography (N Kawasegi et al.); Nanowire Assembly and Integration (Z Gu & D H Gracias); Extreme Ultraviolet Lithography (H Kinoshita); Electron Projection Lithography (T Miura et al.); Electron Beam Direct Writing (K Yamazaki); Electron Beam Induced Deposition (K Mitsuishi); Focused Ion Beams and Interaction with Solids (T Ishitani et al.); Nanofabrication of Nanoelectromechanical Systems (NEMS): Emerging Techniques (K L Ekinci & J Brugger); and other papers. Readership: Researchers, professionals, and graduate students in the fields of nanoengineering and nanoscience.
| Author | : Zhi Li |
| Publisher | : |
| Total Pages | : |
| Release | : 2005 |
| Genre | : |
| ISBN | : |
This dissertation describes a series of experiments aimed at developing new synthetic methods towards DNA-modified nanostructured materials, and it also explores new schemes for the 'bio-inspired' rational assembly of such materials.
| Author | : Gang Wei |
| Publisher | : MDPI |
| Total Pages | : 134 |
| Release | : 2020-03-25 |
| Genre | : Technology & Engineering |
| ISBN | : 303928536X |
Biomolecular self-assembly provides a green, facile, and highly effective method to synthesize various functional nanomaterials that have exhibited considerable potential in the fields of nanotechnology, materials science, biomedicine, tissue engineering, food science, energy storage, and environmental science. In this collection of articles, we presented recent advance in the synthesis, characterization, and applications of self-assembled bio-nanomaterials. In a comprehensive review article, the controlled self-assembly of biomolecules including DNA, protein, peptide, enzymes, virus, and biopolymers via internal interactions and external simulations is introduced and discussed in detail. In other research articles, the self-assembly of DNA, protein, peptide, bio-drugs, liquid crystal polycarbonates, and diblock copolymers to various biomimetic/bioinspired nanomaterials and their potential applications in nanopatterning, sensors/biosensors, drug delivery, anti-parasite, and water purification are demonstrated.
| Author | : Charles M. Lukehart |
| Publisher | : John Wiley & Sons |
| Total Pages | : 1405 |
| Release | : 2013-02-19 |
| Genre | : Technology & Engineering |
| ISBN | : 1118625226 |
Connecting inorganic chemistry to the hottest topic in materials science, this timely resource collects the contributions made by leading inorganic chemists towards nanomaterials research. The second volume in the “Wiley Encyclopedia of Inorganic Chemistry Methods and Applications Series,” this signature title concentrates on recent developments in the field and includes all key topics such as nanowires, nanotubes, biomineralization, supramolecular materials and much more. This volume is also available as part of Encyclopedia of Inorganic Chemistry, 5 Volume Set. This set combines all volumes published as EIC Books from 2007 to 2010, representing areas of key developments in the field of inorganic chemistry published in the Encyclopedia of Inorganic Chemistry. Find out more.
