Current Protocols In Protein Science Supplement 25
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Author | : Sean R. Gallagher |
Publisher | : John Wiley & Sons |
Total Pages | : 679 |
Release | : 2012-03-19 |
Genre | : Science |
ISBN | : 047094241X |
The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.
Author | : Haian Fu |
Publisher | : Cambridge University Press |
Total Pages | : 359 |
Release | : 2012-02-13 |
Genre | : Medical |
ISBN | : 0521889480 |
This text covers new techniques and applications in chemical genomics for researchers, professionals and graduates in biology, biomedicine and chemistry.
Author | : Suzanne Nielsen |
Publisher | : Springer Science & Business Media |
Total Pages | : 585 |
Release | : 2014-09-04 |
Genre | : Technology & Engineering |
ISBN | : 1441914773 |
This book provides information on the techniques needed to analyze foods in laboratory experiments. All topics covered include information on the basic principles, procedures, advantages, limitations, and applications. This book is ideal for undergraduate courses in food analysis and is also an invaluable reference to professionals in the food industry. General information is provided on regulations, standards, labeling, sampling and data handling as background for chapters on specific methods to determine the chemical composition and characteristics of foods. Large, expanded sections on spectroscopy and chromatography also are included. Other methods and instrumentation such as thermal analysis, ion-selective electrodes, enzymes, and immunoassays are covered from the perspective of their use in the analysis of foods. A website with related teaching materials is accessible to instructors who adopt the textbook.
Author | : John E. Coligan |
Publisher | : Wiley-Blackwell |
Total Pages | : 832 |
Release | : 2003-10-24 |
Genre | : Science |
ISBN | : |
Short Protocols in Protein Science provides condensed descriptions of more than 500 protocols compiled from Current Protocols in Protein Science. Drawing from both the original "core" manual as well as the quarterly update service, this compendium includes all step-by-step descriptions of the principal methods covered in Current Protocols in Protein Science.
Author | : S. Suzanne Nielsen |
Publisher | : Springer |
Total Pages | : 648 |
Release | : 2017-06-06 |
Genre | : Technology & Engineering |
ISBN | : 3319457764 |
This fifth edition provides information on techniques needed to analyze foods for chemical and physical properties. The book is ideal for undergraduate courses in food analysis and is also an invaluable reference to professionals in the food industry. General information chapters on regulations, labeling, sampling, and data handling provide background information for chapters on specific methods to determine chemical composition and characteristics, physical properties, and objectionable matter and constituents. Methods of analysis covered include information on the basic principles, advantages, limitations, and applications. Sections on spectroscopy and chromatography along with chapters on techniques such as immunoassays, thermal analysis, and microscopy from the perspective of their use in food analysis have been expanded. Instructors who adopt the textbook can contact the editor for access to a website with related teaching materials.
Author | : Dermot Walls |
Publisher | : Humana Press |
Total Pages | : 527 |
Release | : 2011-04-06 |
Genre | : Science |
ISBN | : 9781607619147 |
A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular BiologyTM series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.
Author | : Frederick M. Ausubel |
Publisher | : |
Total Pages | : 1020 |
Release | : 1987 |
Genre | : Molecular biology |
ISBN | : |
Author | : |
Publisher | : |
Total Pages | : |
Release | : 1996 |
Genre | : Proteins |
ISBN | : |
Author | : Debmalya Barh |
Publisher | : CRC Press |
Total Pages | : 609 |
Release | : 2016-04-19 |
Genre | : Medical |
ISBN | : 1439850097 |
A reflection of the explosion of research and development in this field, OMICS: Biomedical Perspectives and Applications explores applications of omics in bioinformatics, cancer research and therapy, diabetes research, plant science, molecular biology, and neurosciences. A select editorial panel of experts discusses their cutting edge omics researc
Author | : Stephen H. Powis |
Publisher | : Springer Science & Business Media |
Total Pages | : 343 |
Release | : 2008-02-05 |
Genre | : Medical |
ISBN | : 1592592910 |
The aim of MHC Protocols is to document protocols that can be used for the analysis of genetic variation within the human major histocompatibility complex (MHC; HLA region). The human MHC encompasses approximately 4 million base pairs on the short arm of chromosome 6 at cytogenetic location 6p21. 3. The region is divided into three subregions. The telomeric class I region contains the genes that encode the HLA class I molecules HLA-A, -B, and -C. The centromeric class II region contains the genes encoding the HLA class II molecules HLA-DR, -DQ, and -DP. In between is the class III region, originally identified because it contains genes encoding components of the complement pathway. The entire human MHC has recently been sequenced (1) and each subregion is now known to contain many other genes, a number of which have immunological functions. The study of polymorphism within the MHC is well established, because the region contains the highly polymorphic HLA genes. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. As a result, large numbers of HLA alleles have been identified, a process that has been further driven by recent interest in HLA gene diversity in ethnic populations. The extreme genetic variation in HLA genes is believed to have been driven by the evolutionary response to infectious agents, but relatively few studies have analyzed associations between HLA genetic variation and infectious disease, which has been difficult to demonstrate.