Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques
Author: Sean R. Gallagher
Publisher: John Wiley & Sons
Total Pages: 679
Release: 2012-03-19
Genre: Science
ISBN: 047094241X

The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Strengthening Forensic Science in the United States

Strengthening Forensic Science in the United States
Author: National Research Council
Publisher: National Academies Press
Total Pages: 348
Release: 2009-07-29
Genre: Law
ISBN: 0309142393

Scores of talented and dedicated people serve the forensic science community, performing vitally important work. However, they are often constrained by lack of adequate resources, sound policies, and national support. It is clear that change and advancements, both systematic and scientific, are needed in a number of forensic science disciplines to ensure the reliability of work, establish enforceable standards, and promote best practices with consistent application. Strengthening Forensic Science in the United States: A Path Forward provides a detailed plan for addressing these needs and suggests the creation of a new government entity, the National Institute of Forensic Science, to establish and enforce standards within the forensic science community. The benefits of improving and regulating the forensic science disciplines are clear: assisting law enforcement officials, enhancing homeland security, and reducing the risk of wrongful conviction and exoneration. Strengthening Forensic Science in the United States gives a full account of what is needed to advance the forensic science disciplines, including upgrading of systems and organizational structures, better training, widespread adoption of uniform and enforceable best practices, and mandatory certification and accreditation programs. While this book provides an essential call-to-action for congress and policy makers, it also serves as a vital tool for law enforcement agencies, criminal prosecutors and attorneys, and forensic science educators.

Neuroscience Databases

Neuroscience Databases
Author: Rolf Kötter
Publisher: Springer Science & Business Media
Total Pages: 340
Release: 2003
Genre: Computers
ISBN: 9781402071652

Neuroscience Databases: A Practical Guide is the first book providing a comprehensive overview of these increasingly important databases. This volume makes the results of the Human Genome Project and other recent large-scale initiatives in the neurosciences available to a wider community. It extends the scope of bioinformatics from the molecular to the cellular, microcircuitry and systems levels, dealing for the first time with complex neuroscientific issues and leading the way to a new culture of data sharing and data mining necessary to successfully tackle neuroscience questions. Aimed at the novice user who wants to access the data, it provides clear and concise instructions on how to download the available data sets and how to use the software with a minimum of technical detail with most chapters written by the database creators themselves. Key databases and topics include: -Neuroinformatics for C. Elegans; -Gene Expression Patterns; -Functional Analyses of Olfactory Receptors -Protein-Protein Interactions; -Web-Based Neuronal Archives; -Neuronal and Network Modeling; -Storage and Retrieval of Experimental Data for Biophysically Realistic Modeling; -Analysis of Spike Trains; -Neural Connectivity Patterns; -Software Tools for Neuroimaging; -Data Management, Inspection and Sharing.

ROAR

ROAR
Author: Stacy T. Sims, PhD
Publisher: Rodale Books
Total Pages: 306
Release: 2016-07-05
Genre: Health & Fitness
ISBN: 1623366879

“Dr. Sims realizes that female athletes are different than male athletes and you can’t set your race schedule around your monthly cycle. ROAR will help every athlete understand what is happening to her body and what the best nutritional strategy is to perform at her very best.”—Evie Stevens, Olympian, professional road cyclist, and current women’s UCI Hour record holder Women are not small men. Stop eating and training like one. Because most nutrition products and training plans are designed for men, it’s no wonder that so many female athletes struggle to reach their full potential. ROAR is a comprehensive, physiology-based nutrition and training guide specifically designed for active women. This book teaches you everything you need to know to adapt your nutrition, hydration, and training to your unique physiology so you can work with, rather than against, your female physiology. Exercise physiologist and nutrition scientist Stacy T. Sims, PhD, shows you how to be your own biohacker to achieve optimum athletic performance. Complete with goal-specific meal plans and nutrient-packed recipes to optimize body composition, ROAR contains personalized nutrition advice for all stages of training and recovery. Customizable meal plans and strengthening exercises come together in a comprehensive plan to build a rock-solid fitness foundation as you build lean muscle where you need it most, strengthen bone, and boost power and endurance. Because women’s physiology changes over time, entire chapters are devoted to staying strong and active through pregnancy and menopause. No matter what your sport is—running, cycling, field sports, triathlons—this book will empower you with the nutrition and fitness knowledge you need to be in the healthiest, fittest, strongest shape of your life.

Heterologous Expression of Membrane Proteins

Heterologous Expression of Membrane Proteins
Author: Isabelle Mus-Veteau
Publisher: Springer Nature
Total Pages: 472
Release: 2022-06-30
Genre: Science
ISBN: 1071623680

This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.

Aspergillus Fumigatus and Aspergillosis

Aspergillus Fumigatus and Aspergillosis
Author: Jean-Paul Latgé
Publisher:
Total Pages: 612
Release: 2009
Genre: Science
ISBN:

Offers the latest insights into the fundamental biology and pathogenesis of A. fumigatus. Provides a combined synopsis of both A. fumigatus and its diseases and therapies. Encompasses the most up-to-date knowledge to serve as a resource guide for the next decade of study on this organism and the many diseases it causes. Covers the fundamental biology of A. fumigatus including specific features in genetics, biochemistry, and cell biology that can explain the virulence of this opportunistic pathogen. Discusses the wide range of clinical infection, plus the latest diagnostic and treatment strategies, in specific patient populations.