CRISPR-Cas System in Translational Biotechnology

CRISPR-Cas System in Translational Biotechnology
Author: Swati Joshi
Publisher: Elsevier
Total Pages: 317
Release: 2023-11-16
Genre: Science
ISBN: 0323972284

CRISPR-Cas System in Translational Biotechnology discusses applied and translational aspects of the CRISPR-Cas technology. The book bridges the gap between theoretical knowledge and practical solutions surrounding this emerging and impactful technology in several academic and industrial fields. It is split in five sections: CRISP-Cas fundamentals and advancements; CRISP-Cas in medical biotechnology; CRISP-Cas in environmental biotechnology; CRISP-Cas in food biotechnology; and biosafety, patents and commercialization of CRISP-Cas technology. Written by experts from diverse backgrounds, the content covers the subject and its impact in multiple fields. It is a valuable resource for graduate students and researchers on bioinformatics, systems biology, and members of the biomedical field and biotechnology industry who are interested in learning more about CRISP-Cas system and its applications. - Discusses applied aspects of CRISPR-Cas technology and state-of-the-art technological/translational advancements in the field - Focuses on the CRISPR-Cas mediated genetic engineering for employment in various industries such as medical, agricultural, environmental and food - Encompasses knowledge on CRISPR-Cas commercialization, potential markets and associated ethical challenges

CRISPR

CRISPR
Author: Rodolphe Barrangou
Publisher: John Wiley & Sons
Total Pages: 308
Release: 2022-09-19
Genre: Science
ISBN: 1683673611

“An excellent compendium of all things CRISPR from some of the leading minds in the field. With thorough coverage from every angle and beautifully detailed illustrations, this book is not to be missed!” Jennifer A. Doudna, Professor of Chemistry, Biochemistry & Molecular Biology, UC Berkeley; Founder, Innovative Genomics Institute; Nobel Laureate and coinventor of CRISPR technology “This journey through CRISPR biology and several of its breakthrough applications offers an exciting glimpse into one of the most beautiful and compelling fields in the life sciences.” David R. Liu, Director of the Merkin Institute at the Broad Institute of MIT and Harvard; Professor of Chemistry and Chemical Biology at Harvard University; coinventor of base editing and prime editing “A must read! The CRISPR topics, written by world-leading experts, span from the fascinating mechanistic underpinnings to the ingenious applications. One can read from start to finish or pick and choose themes. Either way, the book delivers utterly enjoyable learning!” Bonnie Bassler, Squibb Professor and Chair, Princeton University Department of Molecular Biology; Howard Hughes Medical Institute Investigator CRISPR-Cas systems have revolutionized the science of gene editing and their possible applications continue to expand, from basic research to potentially groundbreaking medical and commercial uses. Led by a distinguished team of editors, CRISPR: Biology and Applications explores the subject matter needed to delve into this fascinating area. Topics covered include: Classification and molecular mechanisms of CRISPR-Cas systems CRISPR-Cas evolution, regulation, expression, and function Uses for gene editing and modulation of gene expression CRISPR-based antimicrobials and phage resistance for medical and industrial purposes Written by internationally renowned authors, CRISPR: Biology and Applications serves as both an introductory guide for those new to the field and an authoritative reference for seasoned researchers whose work touches this evolving and headline- making science.

CRISPR People

CRISPR People
Author: Henry T. Greely
Publisher: MIT Press
Total Pages: 395
Release: 2022-03-01
Genre: Technology & Engineering
ISBN: 0262543885

What does the birth of babies whose embryos had gone through genome editing mean--for science and for all of us? In November 2018, the world was shocked to learn that two babies had been born in China with DNA edited while they were embryos—as dramatic a development in genetics as the 1996 cloning of Dolly the sheep. In this book, Hank Greely, a leading authority on law and genetics, tells the fascinating story of this human experiment and its consequences. Greely explains what Chinese scientist He Jiankui did, how he did it, and how the public and other scientists learned about and reacted to this unprecedented genetic intervention. The two babies, nonidentical twin girls, were the first “CRISPR'd” people ever born (CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats, is a powerful gene-editing method). Greely not only describes He's experiment and its public rollout (aided by a public relations adviser) but also considers, in a balanced and thoughtful way, the lessons to be drawn both from these CRISPR'd babies and, more broadly, from this kind of human DNA editing—“germline editing” that can be passed on from one generation to the next. Greely doesn't mince words, describing He's experiment as grossly reckless, irresponsible, immoral, and illegal. Although he sees no inherent or unmanageable barriers to human germline editing, he also sees very few good uses for it—other, less risky, technologies can achieve the same benefits. We should consider the implications carefully before we proceed.

CRISPR-Cas Systems

CRISPR-Cas Systems
Author: Rodolphe Barrangou
Publisher: Springer Science & Business Media
Total Pages: 300
Release: 2012-12-13
Genre: Science
ISBN: 364234657X

CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.

Enzymes in Synthetic Biology

Enzymes in Synthetic Biology
Author:
Publisher: Academic Press
Total Pages: 460
Release: 2018-08-30
Genre: Science
ISBN: 0128151498

Volume 608 of the series Methods in Enzymology covers key aspects of enzyme discovery, engineering tools and platforms, and examples of applications in the enzymology of synthetic biology. Detailed methods for laboratory use of enzymes in synthetic biology applications Informative case history examples illustrating how enzyme and metabolic engineering are used to generate new products Emphasises latest developments in laboratory automation for the engineering of biology Covers many aspects of the design, build, test, learn cycle used in synthetic biology

CRISPR'd

CRISPR'd
Author: Judy Foreman
Publisher: Simon and Schuster
Total Pages: 202
Release: 2022-02-15
Genre: Fiction
ISBN: 1510770356

For fans of Julia Buckley and Tess Gerritsen, a debut featuring a killer in plain sight using a microscopic murder weapon, the cutting edge gene-editing technology: CRISPR. Boston geneticist Dr. Saul Kramer is on the cutting edge of genetic disease research. Revered among clients at his IVF clinic, he harbors a dark secret. In addition to helping infertile couples conceive healthy babies, Dr. Kramer is obsessed, for his own dark reasons, with an alternate mission as well. In certain patients, he uses the gene editing technology CRISPR to tamper with embryos, not to improve the health of the embryos, but to replace a healthy gene with a deadly mutation. A young female journalist, Sammie Fuller, begins to suspect what he has done when three infants conceived at his clinic die mysteriously, all at about one year old. She and a molecular biologist work secretly in his MIT lab to identify any genetic defects in the deceased children and together make a chilling discovery. Thanks to Sammie’s blockbuster stories, which go viral, Dr. Kramer is charged with murder and winds up in court. In the subsequent dramatic court scenes, his feisty defense lawyer stuns the world with her defense. Set in this uneasy time of genetic engineering with CRISPR technology, Foreman, spins a compelling tale of love, revenge, and murder.

Engineering Biology for Microbial Biosynthesis of Plant-Derived Bioactive Compounds

Engineering Biology for Microbial Biosynthesis of Plant-Derived Bioactive Compounds
Author: Yongjun Wei
Publisher: Elsevier
Total Pages: 383
Release: 2024-03-01
Genre: Science
ISBN: 0443155593

Many plant-derived bioactive compounds are the foundation effective drugs to cure diseases. Usually, the bioactive compounds in plant biomass are low, and the extraction of bioactive compounds from plants is not eco-friendly, which limits their application in drugs and other industries. With the development of synthetic biology and green biomanufacturing, the biosynthesis of complex bioactive compounds is possible. However, the engineering fundamentals for microbial biosynthetic of plant-derived bioactive compounds is vast. Engineering biology for microbial biosynthesis of plant-derived bioactive compounds summarizes the engineering fundamental biotechnologies for microbial production of plant-derived bioactive compounds, allowing this content to be easily accessed. Coverage includes summaries of key achievements, identification of challenges, current approaches, tips and tricks, and helpful insights into critical bottlenecks. With a comprehensive coverage of the field, Engineering biology for microbial biosynthesis of plant-derived bioactive compounds is of great interest to undergraduate and graduate students, scientists, researchers as well as engineers who work on the microbial production of plant-derived bioactive compounds. - Provides valuable information for engineers, scientists, and industrial researchers who want to obtain microbial strains with the ability of plant-derived bioactive compound production - Guides microbial production of plant-derived bioactive compounds at the laboratory and industrial levels - Ideal resource for anyone who works on the microbial production of plant-derived bioactive compounds