The document contains food additive specification monographs, analytical methods, and other information prepared at the ninety-fifth meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), which was held virtually from 6 to 17 June and 22 June 2022. The specification monographs provide information on the identity and purity of food additives used directly in foods or in food production. The main three objectives of these specifications are to identify the food additive that has been subjected to testing for safety, to ensure that the additives are of the quality required for use in food or in processing and to reflect and encourage good manufacturing practice. This publication and other documents produced by JECFA contain information that is useful to all those who work with or are interested in food additives and their safe use in food. At its 95th meeting, JECFA developed full specifications for one processing aid (lipase from Thermomyces lanuginosus and Fusarium Oxysporum expressed in Aspergillus oryzae (JECFA95-7)) and two flavouring agents (trans-4-tert-butylcyclohexanol (2263), caryophylla-3(4), 8-dien-5-ol (2264)). JECFA revised the specifications for spirulina extract (INS 134). Moreover, JECFA developed tentative specifications for seven processing aids (?-amylase from Geobacillus stearothermophilus expressed in Bacillus licheniformis (JECFA95-1), ?-amylase from Geobacillus stearothermophilus expressed in Bacillus licheniformis (JECFA95-2), ?-amylase from Rhizomucor pusillus expressed in Aspergillus niger (JECFA95 3), amyloglucosidase from Rasamsonia emersonii expressed in Aspergillus niger (JECFA95-4), asparaginase from Pyrococcus furiosus expressed in Bacillus subtilis (JECFA95-5), ?-amylase from Bacillus flexus expressed in Bacillus licheniformis (JECFA95-6), xylanase from Bacillus licheniformis expressed in Bacillus licheniformis (JECFA95-9)).