Colorimetric Determination of Nitrate Plus Nitrite in Water by Enzymatic Reduction, Automated Discrete Analyzer Methods

Colorimetric Determination of Nitrate Plus Nitrite in Water by Enzymatic Reduction, Automated Discrete Analyzer Methods
Author: Charles J Patton
Publisher: CreateSpace
Total Pages: 44
Release: 2014-06-16
Genre: Technology & Engineering
ISBN: 9781500223311

This report documents work at the U.S. Geological Sur- vey (USGS) National Water Quality Laboratory (NWQL) to validate enzymatic reduction, colorimetric determinative meth- ods for nitrate + nitrite in filtered water by automated discrete analysis. In these standard- and low-level methods (USGS I-2547-11 and I-2548-11), nitrate is reduced to nitrite with nontoxic, soluble nitrate reductase rather than toxic, granular, copperized cadmium used in the longstanding USGS auto- mated continuous-flow analyzer methods I-2545-90 (NWQL laboratory code 1975) and I-2546-91 (NWQL laboratory code 1979). Colorimetric reagents used to determine resulting nitrite in aforementioned enzymatic- and cadmium-reduction meth- ods are identical. The enzyme used in these discrete analyzer methods, designated AtNaR2 by its manufacturer, is produced by recombinant expression of the nitrate reductase gene from wall cress (Arabidopsis thaliana) in the yeast Pichia pastoris. Unlike other commercially available nitrate reductases we evaluated, AtNaR2 maintains high activity at 37°C and is not inhibited by high-phenolic-content humic acids at reaction temperatures in the range of 20°C to 37°C. These previously unrecognized AtNaR2 characteristics are essential for success- ful performance of discrete analyzer nitrate + nitrite assays (henceforth, DA-AtNaR2) described here.

Soil Sampling and Methods of Analysis

Soil Sampling and Methods of Analysis
Author: M.R. Carter
Publisher: CRC Press
Total Pages: 1264
Release: 2007-08-03
Genre: Science
ISBN: 1420005278

Thoroughly updated and revised, this second edition of the bestselling Soil Sampling and Methods of Analysis presents several new chapters in the areas of biological and physical analysis and soil sampling. Reflecting the burgeoning interest in soil ecology, new contributions describe the growing number and assortment of new microbiological

Environmental Chemical Analysis

Environmental Chemical Analysis
Author: S. Mitra
Publisher: CRC Press
Total Pages: 345
Release: 2018-10-03
Genre: Science
ISBN: 1482231638

The study of the environment requires the reliable and accurate measurement of extremely small quantities of chemicals and the ability to determine if they are pollutants or naturally occurring species. Historically, a "dilute and disperse" method of waste disposal has been accepted; yet as we learn the long-term consequences of such an approach, it is clear that more rigorous waste management techniques are necessary to understand the sources and fates of contaminants and to regulate their discharge. This volume presents the details of the basic analytical science involved in making these measurements. It concentrates on the basic principles of sampling and sample preparation, followed by the chemical principles of the major instrumental methods used in chemical analysis, and detailed discussions of the major environmental matrices. This book also provides coverage of topics usually only partially discussed in textbooks, such as quality assurance plans and statistical data handling. Students majoring in environmental sciences need a foundation in measurement techniques used in the field. Environmental Chemical Analysis gives students a thorough grounding in this field and enough information to judge the quality and interpret the information produced in the analytical laboratory.

Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms

Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms
Author: Horacio G Pontis
Publisher: Academic Press
Total Pages: 252
Release: 2016-09-06
Genre: Science
ISBN: 0128034033

Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth to them. Chapters include information on how biological active protein is extracted for different cells, determination of enzymatic activity, separation of proteins by different available methods, and descriptions of analytical methods for the determination of various types of carbohydrates in photosynthetic organisms. The book contains useful protocols for researchers working on the determination of carbohydrate metabolism. The book provides foundational content as well as step-by-step guidance on how to design and conduct an experiment, including what other methodologies could be used if advanced instruments are not readily available. - Includes a variety of analytical methods and how to apply the methods using examples from specific case studies - Discusses technical information on how to characterize plant carbohydrates and sugar nucleosides - Contains easy-to-follow protocols with detailed explanations for self-guidance - Provides foundational content as well as step-by-step guidance on how to design and conduct an experiment

Trace Elemental Analysis of Metals

Trace Elemental Analysis of Metals
Author: Thomas R. Dulski
Publisher: Routledge
Total Pages: 602
Release: 2017-10-06
Genre: Science
ISBN: 135140735X

This work details minor, trace and ultratrace methods; addresses the essential stages that precede measurement; and highlights the measurement systems most likey to be used by the pragmatic analyst. It features key material on inclusion and phase isolation. The book is designed to provide useful maps and signposts for metals analysts who must verify that stringent trace level compositional specifications have been met.

Chemical Analysis

Chemical Analysis
Author: Louis Klein
Publisher: Elsevier
Total Pages: 217
Release: 2016-01-22
Genre: Science
ISBN: 1483162591

River Pollution 1: Chemical Analysis discusses methods of detecting and determining the various forms of pollution and the interpretation of results. It aims to provide a chemical background for, and supplement to, the information on analytical methods, and to review critically other methods which may be useful in certain circumstances for research, control work, and field tests. The book begins with a description of river surveys, and physical and chemical methods for determining river pollution. Separate chapters cover methods to determine the presence of dissolved oxygen, combined nitrogen, sulfur compounds, carbon dioxide, free chlorine, metallic contaminants, and carbon compounds. Subsequent chapters discuss the estimation of less important substances which may sometimes be encountered in pollution problems; and the significance of chemical and physical tests and the interpretation of the results of an analysis. This book is intended for those interested in chemical analysis as applied to river pollution problems, sewage, and trade wastes.

Project Report

Project Report
Author: USAF School of Aerospace Medicine
Publisher:
Total Pages: 724
Release: 1960
Genre:
ISBN:

Handbook of Green Analytical Chemistry

Handbook of Green Analytical Chemistry
Author: Miguel de la Guardia
Publisher: John Wiley & Sons
Total Pages: 569
Release: 2012-02-23
Genre: Science
ISBN: 111994094X

The emerging field of green analytical chemistry is concerned with the development of analytical procedures that minimize consumption of hazardous reagents and solvents, and maximize safety for operators and the environment. In recent years there have been significant developments in methodological and technological tools to prevent and reduce the deleterious effects of analytical activities; key strategies include recycling, replacement, reduction and detoxification of reagents and solvents. The Handbook of Green Analytical Chemistry provides a comprehensive overview of the present state and recent developments in green chemical analysis. A series of detailed chapters, written by international specialists in the field, discuss the fundamental principles of green analytical chemistry and present a catalogue of tools for developing environmentally friendly analytical techniques. Topics covered include: Concepts: Fundamental principles, education, laboratory experiments and publication in green analytical chemistry. The Analytical Process: Green sampling techniques and sample preparation, direct analysis of samples, green methods for capillary electrophoresis, chromatography, atomic spectroscopy, solid phase molecular spectroscopy, derivative molecular spectroscopy and electroanalytical methods. Strategies: Energy saving, automation, miniaturization and photocatalytic treatment of laboratory wastes. Fields of Application: Green bioanalytical chemistry, biodiagnostics, environmental analysis and industrial analysis. This advanced handbook is a practical resource for experienced analytical chemists who are interested in implementing green approaches in their work.