Chromatography Of Steroids
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| Author | : Erich Heftmann |
| Publisher | : Elsevier |
| Total Pages | : 558 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323146449 |
Modern Methods of Steroid Analysis reviews modern methods of steroid analysis such as liquid column chromatography, mass spectrometry, and gas chromatography. Topics covered include qualitative and quantitative analysis of plant sterols by gas-liquid chromatography; Raman spectroscopy of steroids; nuclear magnetic resonance; and applications of lanthanide shift reagents. A computerized method for rapid comparison and retrieval of infrared spectral data is also described. This volume is comprised of 22 chapters and begins with an analysis of hormonal steroids using liquid column chromatography, followed by a discussion on the separation of insect molting hormones using high-pressure liquid chromatography. The reader is methodically introduced to the application of gradient elution and thin-layer chromatography to the analysis of corticosteroids and 17-ketosteroids, as well as some aspects of mass spectrometry in steroid analysis. Subsequent chapters explore other methods such as optical rotatory dispersion and circular dichroism, along with radioisotope techniques. In particular, the radioimmunoassay of plasma steroid hormones and plasma aldosterone is considered. This book should be of interest to biochemists.
| Author | : Kristen B. Eik-Nes |
| Publisher | : Springer |
| Total Pages | : 0 |
| Release | : 2012-05-27 |
| Genre | : Science |
| ISBN | : 9783642867958 |
Few fields have advanced faster over the past quinquennium than separation and estimation of steroids by the technique of gas phase chromatography. A detailed and complete review of this topic would therefore be beyond the scope of the authors contributing to this monograph. It was, however, felt that a discussion of some of the highlights of this rapid advance might be of help for laboratories estimating steroids in biological samples. One of the difficulties in producing a monograph of this kind is that before it can appear in print it is likely that some of the methods it discusses will have been overtaken by better methods, so swiftly is progress now made. No editorial power has been exercised in trying to make a uni form account of technology in this field, and the idiosyncrasies of each individual author have been left intact. Through this approach we hope that what has been lost in scholarly appearance is regained in general appeal.
| Author | : Ian Elcock Bush |
| Publisher | : Pergamon |
| Total Pages | : 474 |
| Release | : 1961 |
| Genre | : Medical |
| ISBN | : |
The basic theory of chromatography and some general principles; Chromatographic separation of steroids; Techniques and apparatus; Quantitative chromatography: colorimetric and radioisotopic techniques; Structural analysis and identification of steroids by chromatography; Some typical analytical problems of steroid biochemistry; Purification of reagents and materials; Microchemical reactions for steroids; Methods of detection on paper chromatograms; Calculations with Rf and Rm value.
| Author | : Joseph Sherma |
| Publisher | : CRC Press |
| Total Pages | : 1356 |
| Release | : 2003-04-18 |
| Genre | : Science |
| ISBN | : 0824748662 |
In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.
| Author | : Donald H. Chace |
| Publisher | : |
| Total Pages | : 97 |
| Release | : 2007 |
| Genre | : Diagnosis, Laboratory |
| ISBN | : 9781562386481 |
| Author | : Richard J. Block |
| Publisher | : Elsevier |
| Total Pages | : 208 |
| Release | : 2013-09-03 |
| Genre | : Science |
| ISBN | : 1483276872 |
Paper Chromatography: A Laboratory Manual focuses on methods, technologies, and processes, and aims to provide readers with a readily accessible source for the uses and adaptations of paper chromatography. The book first offers information on general methods, including descending, ascending, and ascending-descending chromatography, filter paper ""chromatopile"", ""reversed phase"" paper chromatography, and paper electrophoresis. The text then elaborates on quantitative methods and amino acids, amines, and proteins. Discussions focus on visual comparison, elution, area of spot, total color of spot, maximum color density, identification of amines, separation of proteins, and general directions. The publication examines carbohydrates and aliphatic acids and steroids. Topics include simple sugars, miscellaneous derived sugars, and aliphatic acids. The text also ponders on purines, pyrimidines, and related substances and phenols, aromatic acids, and porphyrins. The text is a valuable reference for readers interested in paper chromatography.
| Author | : Bruce Spiegelman |
| Publisher | : Springer |
| Total Pages | : 108 |
| Release | : 2018-03-07 |
| Genre | : Science |
| ISBN | : 3319727907 |
The world is faced with an epidemic of metabolic diseases such as obesity and type 2 diabetes. This is due to changes in dietary habits and the decrease in physical activity. Exercise is usually part of the prescription, the first line of defense, to prevent or treat metabolic disorders. However, we are still learning how and why exercise provides metabolic benefits in human health. This open access volume focuses on the cellular and molecular pathways that link exercise, muscle biology, hormones and metabolism. This will include novel “myokines” that might act as new therapeutic agents in the future.
| Author | : Jack Cazes |
| Publisher | : CRC Press |
| Total Pages | : 2968 |
| Release | : 2009-10-12 |
| Genre | : Science |
| ISBN | : 1439870675 |
Thoroughly revised and expanded, this third edition offers illustrative tables and figures to clarify technical points in the articles and provides a valuable, reader-friendly reference for all those who employ chromatographic methods for analysis of complex mixtures of substances. An authoritative source of information, this introductory guide to specific chromatographic techniques and theory discusses the relevant science and technology, offering key references for analyzing specific chemicals and applications in industry and focusing on emerging technologies and uses.
| Author | : Wilfried M.A. Niessen |
| Publisher | : CRC Press |
| Total Pages | : 629 |
| Release | : 2006-08-09 |
| Genre | : Medical |
| ISBN | : 1420014544 |
A constructive evaluation of the most significant developments in liquid chromatography-mass spectrometry (LC-MS) and its uses for quantitative bioanalysis and characterization for a diverse range of disciplines, Liquid Chromatography-Mass Spectrometry, Third Edition offers a well-rounded coverage of the latest technological developments and
| Author | : Roger Bertholf |
| Publisher | : John Wiley & Sons |
| Total Pages | : 308 |
| Release | : 2007-01-30 |
| Genre | : Science |
| ISBN | : 0470023104 |
This book aims to fill the gap that exists between theoretical treatments of chromatography, and clinical chemistry and toxicology texts, which focus almost exclusively on clinical relevance and applications. Chromatography has a vast array of clinical applications, and though the chromatographic methods were first introduced decades ago, new applications of this technology are being used to explore previously inaccessible frontiers in clinical diagnostics and toxicological testing. An up-to-date book devoted to clinical and toxicological applications of chromatographic methods will serve as an instructional and reference text, useful to students, laboratory technicians, and researchers.
