Chromatography For The Analysis Of Lipids
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Author | : William W. Christie |
Publisher | : Woodhead Publishing Limited |
Total Pages | : 328 |
Release | : 1989 |
Genre | : Science |
ISBN | : |
Fatty acids and lipids: structures, extraction and fractionation into classes -- Gas chromatography: theoretical aspects and instrumentation -- Preparation of methyl ester and other derivatives -- Gas chromatographic analysis of fatty acid derivatives -- Isolation of fatty acids and identification by spectroscopic and chemical degradative techniques -- Gas chromatography--mass spectrometry and fatty acids -- Gas chromatographic analysis of molecular species of lipids -- Alternative or complementary methods for the analysis of molecular species of lipids -- Some miscellaneous separations of lipids by gas chromatography.
Author | : W. W. Christie |
Publisher | : Elsevier |
Total Pages | : 447 |
Release | : 2010-01-10 |
Genre | : Technology & Engineering |
ISBN | : 0857097865 |
This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and ‘lipidomics’ as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf. Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics. The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.
Author | : Hans-Ferdinand Linskens |
Publisher | : Springer Science & Business Media |
Total Pages | : 267 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 3642829511 |
Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itselfwith little need to consult other publications. Contributing authors have attempted to follow these guidelines in this New Series of volumes.
Author | : R.J. Hamilton |
Publisher | : Springer Science & Business Media |
Total Pages | : 406 |
Release | : 2012-12-06 |
Genre | : Technology & Engineering |
ISBN | : 1461311314 |
This book focuses on the developments in the field of lipid analysis, providing an up-to-date review of the analytical techniques available to chemists and technologists to identify complex molecules. The requisite theoretical background will be provided for individual techniques, together with their strengths and weaknesses, and a guide to the enormous range of commercial applications. It will be an invaluable reference source to all sectors of the oils and fats industry where accurate labeling of foods, food contamination and adulteration are issues of increasing interest and concern.
Author | : W. W. Christie |
Publisher | : Elsevier |
Total Pages | : 305 |
Release | : 1997-05-01 |
Genre | : Science |
ISBN | : 0857097997 |
This is the fourth volume of an occasional series of review volumes dealing with aspects of lipid methodology. As with the first three volumes, topics have been selected that have been developing rapidly in recent years and have some importance to lipid analysis. The authors are all leading international experts.Topics covered include: analysis of plant lipoxygenase metabolites, preparative high-performance liquid chromatography of lipids, structural analysis of fatty acids, and analysis of stable isotopes in lipids, among others.
Author | : |
Publisher | : Elsevier |
Total Pages | : 432 |
Release | : 2007-11-26 |
Genre | : Science |
ISBN | : 0080554881 |
This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species within Cells; Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry; Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry; Structure-specific, quantitative methods for "lipidomic" analysis of sphingolipids by tandem mass spectrometry; Analysis of Ubiquinones, Dolichols and Dolichol Diphosphate-Oligosaccharides by Liquid Chromatography Electrospray Ionization Mass Spectrometry; Extraction and Analysis of Sterols in Biological Matrices by High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry; The Lipid Maps Initiative in Lipidomics; Basic analytical systems for lipidomics by mass spectrometry in Japan; The European Lipidomics Initiative Enabling technologies; Lipidomic analysis of Signaling Pathways; Bioinformatics for Lipidomics; Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins and Protectins; A guide to biochemical systems modeling of sphingolipids for the biochemist; and Quantitation and Standardization of Lipid Internal Standards for Mass Spectroscopy.
Author | : William W. Christie |
Publisher | : Pergamon |
Total Pages | : 296 |
Release | : 1987 |
Genre | : Science |
ISBN | : |
A comprehensive guide to lipids and lipid qnalysis using high-performance liquid chromatography. The author covers the construction and occurrence of various lipids and their functions. The book introduces the values of high-performance liquid chromatography, its theoretical considerations and the use of equipment, and discusses the separation of both simple and complex lipid classes. In combining the theoretical and practical sides of lipid anlaysis this book will be of immense value to all those involved in lipid research.
Author | : Dorothea Bartels |
Publisher | : Humana |
Total Pages | : 472 |
Release | : 2022-06-12 |
Genre | : Science |
ISBN | : 9781071613641 |
This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience in lipid experiments who are interested in approaching this field experimentally.
Author | : Michael I. Gurr |
Publisher | : John Wiley & Sons |
Total Pages | : 339 |
Release | : 2008-04-15 |
Genre | : Medical |
ISBN | : 1405172703 |
Since the publication of the first edition of this successful and popular book in 1970, the subject of lipid biochemistry has evolved greatly and this fifth up-to-date and comprehensive edition includes much new and exciting information. Lipid Biochemistry, fifth edition has been largely re-written in a user-friendly way, with chapters containing special interest topic boxes, summary points and lists of suggested reading, further enhancing the accessibility and readability of this excellent text. Contents include abbreviations and definitions used in the study of lipids, routine analytical methods, fatty acid structure and metabolism, dietary lipids and lipids as energy stores, lipid transport, lipids in cellular structures and the metabolism of structural lipids. The book provides a most comprehensive treatment of the subject, making it essential reading for all those working with or studying lipids. Upper level students of biochemistry, biology, clinical subjects, nutrition and food science will find the contents of this book invaluable as a study aid, as will postgraduates specializing in the topics covered in the book. Professionals working in research in academia and industry, including personnel involved in food and nutrition research, new product formulation, special diet formulation (including nutraceuticals and functional foods) and other clinical aspects will find a vast wealth of information within the book's pages. Michael Gurr was a Visiting Professor in Human Nutrition at the University of Reading, UK and at Oxford Brookes University, UK. John Harwood is a Professor of Biochemistry at the School of Biosciences, Cardiff University, UK. Keith Frayn is a Professor of Human Metabolism at the Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, UK.
Author | : Michael T. Arts |
Publisher | : Springer Science & Business Media |
Total Pages | : 333 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 1461205476 |
The fundamental purpose of this book is to synthesise the divergent literature on aquatic lipids into a co-ordinated, digestible form. A large part of the book addresses lipid composition and production in freshwater organisms, with chapters on phytoplankton, zooplankton and benthic invertebrates. A common theme throughout the book is the function of lipids in aquatic food webs, with a chapter devoted exclusively to lipids as indicators of health in fish populations. A complementary chapter highlights the role of lipids and essential fatty acids in mariculture. Methodologies to determine the lipid content of aquatic samples and suggestions as to the utility of fatty acids as trophic markers are included, as is one chapter on the role of lipids in the bioaccumulation and bioconcentration of toxicants and another on the relationships between lipids and surface films and foams. The final chapter highlights the similarities and differences between lipids of marine and freshwater origin. Students and researchers in ecology, phycology, aquatic toxicology, physiological ecology and limnology will find this an invaluable guide and reference.