Protocols for Micropropagation of Woody Trees and Fruits

Protocols for Micropropagation of Woody Trees and Fruits
Author: S.Mohan Jain
Publisher: Springer Science & Business Media
Total Pages: 548
Release: 2007-09-18
Genre: Technology & Engineering
ISBN: 1402063520

Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.

Plant Propagation

Plant Propagation
Author: Hudson Thomas Hartmann
Publisher: Prentice Hall
Total Pages: 760
Release: 1975
Genre: Science
ISBN:

Presents complete coverage of all phases of plant propagation, by seeds, cuttings, grafting, budding, layering, division, and tissue culture propagation.

Micropropagation of Woody Plants

Micropropagation of Woody Plants
Author: M.R. Ahuja
Publisher: Springer Science & Business Media
Total Pages: 510
Release: 2013-06-29
Genre: Science
ISBN: 9401581169

This volume covers recent advances in the vegetative propagation of woody plants by tissue culture. A wide range of topics relevant to micropropagation of woody plants are discussed by renowned international scientists. These include cellular contro of morphogenesis, light regimes in tissue culture, maturation and rejuvenation, synthetic seed, genetics of micropropagated plants, haploid embryogenesis, protoplast culture, and acclimatization of ex vitro woody plants. In addition to micropropagation of selected woody plants, both gymnosperms and angiosperms, this volume also includes in vitro genetic selection, strategic planning for application of biotechnology for genetics and breeding, and clonal options for woody plant improvement. A balanced view of both perspectives and limitations of woody plant micropropagation is presented.

Plant Propagation

Plant Propagation
Author: Hudson Thomas Hartmann
Publisher:
Total Pages: 792
Release: 1997
Genre: Science
ISBN:

Resource added for the Landscape Horticulture Technician program 100014.

Molecular Biology of Woody Plants

Molecular Biology of Woody Plants
Author: S.M. Jain
Publisher: Springer Science & Business Media
Total Pages: 516
Release: 2013-03-09
Genre: Nature
ISBN: 9401723133

Woody plants belong to various taxonomic groups, which are heterogeneous in morphology, physiology, and geographic distribution. OtheJWise, they have neither strong evolutionruy relationships nor share a conunon habitat. They are a primaIy source of fiber and timber, and also include many edible fruit species. Their unique phenotypic behavior includes a perennial habit associated with extensive secondary growth. Additional characteristics of woody plants include: developmental juvenility and maturity with respect to growth habit, flowering time, and morphogenetic response in tissue cultures; environmental control of bud dormancy and flowering cycles; variable tolerance to abiotic stresses, wounding and pathogens; and long distance transport of water and IRltrients. Woody plants, particularly tree species, have been the focus of numerous physiological studies to understand their specialized functions, however, only recently they have become the target of molecular studies. Recent advances in our understanding of signal transduction pathways for environmental responses in herbaceous plants, including the identification and cloning of genes for proteins involved in signal transduction. should provide useful leads to undertake parallel studies with woody plants. Molecular mapping techniques, coupled with the availability of cloned genes from herbaceous plants, should provide shortcuts to cloning relevant genes from woody plants. The unique phenotypes of these plants can then be targeted for improvement through genetic engineering.