Basic Separation Techniques in Biochemistry

Basic Separation Techniques in Biochemistry
Author: R O Okotore
Publisher: New Age International
Total Pages: 108
Release: 1998
Genre: Biotechnology
ISBN: 9788122411706

Basic Separation Techniques In Biochemistry Provides Information On The Basic Separation Techniques Most Commonly Employed In Biochemical Research.The Basic Principles And Applications Of The Routine Methods For The Fractionation Of Subcellular Macromolecules Have Been Discussed In Simple And Comprehensive Manner.The Methodology Of Each Technique Is Presented In A Precise And Concise Way For Meaningful Understanding To A Beginner Student. The Book Is In Eight Chapters, Each With Statement Of Objectives. The Book Will Prove Of Value To Undergraduate Students Of Biochemistry, Chemistry And Biology As Supplementary Reading Text To More Advanced Texts In Laboratory Techniques.

Separation Methods in Organic Chemistry and Biochemistry

Separation Methods in Organic Chemistry and Biochemistry
Author: Frank J. Wolf
Publisher: Elsevier
Total Pages: 246
Release: 2013-10-22
Genre: Science
ISBN: 1483220680

Separation Methods in Organic Chemistry and Biochemistry aims to provide perspectives for the commonly used separations methods and to discuss indications for their use. The book discusses the determination of molecular properties useful in separation based on micro test methods, paper chromatography, thin-layer chromatography, and electrophoresis. The text then describes the theoretical principles of group-separation procedures, liquid-liquid partition, ion-exchange selectivity, gel permeation, and adsorption. Methods of influencing the selectivity coefficients, the basic theory of fractionation methods, and the principles of application are also encompassed. Biochemists and chemists will find the book useful.

Magnetic Cell Separation

Magnetic Cell Separation
Author:
Publisher: Elsevier
Total Pages: 473
Release: 2011-08-31
Genre: Science
ISBN: 0080553508

Cell separation is at the core of current methods in experimental biology and medicine. Its importance is illustrated by the large number of physical and biochemical principles that have been evaluated for application to cell separation. The development of cell separation methods is driven by the needs of biological and medical research, and the ever-increasing demands for sensitivity, selectivity, yield, timeliness and economy of the process. The interdisciplinary nature of research in this area and the volume of information available in research publications and conferences necessitates a basic description of the fundamental processes involved in magnetic cell separation that may help the user in navigating this wealth of information available online and in scientific publications. This book will appeal to researchers in many areas utilizing this technique, including those working in cell biology, clinical research, inorganic chemistry, biochemistry, chemical engineering, materials science, physics and electrical engineering. - Provides examples of how to calculate the volume magnetic susceptibility, a fundamental quantity for calculating the magnetic force acting on a cell, from various types of magnetic susceptibilities available in literature - Introduces the elements of magnetostatics as they apply to cell magnetization and the magnetization of magnetic micro- and nano- particles used for cell separation - Describes the parameters used to determine cell magnetophoresis

Separation Techniques in Clinical Chemistry

Separation Techniques in Clinical Chemistry
Author: Hassan Y. Aboul-Enein
Publisher: CRC Press
Total Pages: 637
Release: 2003-05-28
Genre: Science
ISBN: 1135532788

This reference examines innovations in separation science for improved sensitivity and cost-efficiency, increased speed, higher sample throughput and lower solvent consumption in the assessment, evaluation, and validation of emerging drug compounds. It investigates breakthroughs in sample pretreatment, HPLC, mass spectrometry, capillary electrophor

Separation Methods In Microanalytical Systems

Separation Methods In Microanalytical Systems
Author: Jorg P. Kutter
Publisher: CRC Press
Total Pages: 896
Release: 2005-09-09
Genre: Science
ISBN: 142002793X

Focusing on what has been one of the driving forces behind the development of lab-on-a-chip devices, Separation Methods in Microanalytical Systems explores the implementation, realization, and operation of separation techniques and related complex workflows on microfabricated devices. The book details the design, manufacture, and integration of diverse components needed to perform an entire analytical procedure on a single miniaturized device. This volume is valuable reference for scientists and engineers anticipating the demand for function-specific chemical separation systems in biomedical diagnostics, environmental monitoring, and drug discovery applications.

Separation Techniques Applied to Omics Sciences

Separation Techniques Applied to Omics Sciences
Author: Ana Valéria Colnaghi Simionato
Publisher: Springer Nature
Total Pages: 277
Release: 2021-10-09
Genre: Science
ISBN: 3030772527

This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one.

A Research Agenda for Transforming Separation Science

A Research Agenda for Transforming Separation Science
Author: National Academies of Sciences, Engineering, and Medicine
Publisher: National Academies Press
Total Pages: 115
Release: 2019-10-30
Genre: Science
ISBN: 0309491703

Separation science plays a critical role in maintaining our standard of living and quality of life. Many industrial processes and general necessities such as chemicals, medicines, clean water, safe food, and energy sources rely on chemical separations. However, the process of chemical separations is often overlooked during product development and this has led to inefficiency, unnecessary waste, and lack of consensus among chemists and engineers. A reevaluation of system design, establishment of standards, and an increased focus on the advancement of separation science are imperative in supporting increased efficiency, continued U.S. manufacturing competitiveness, and public welfare. A Research Agenda for Transforming Separation Science explores developments in the industry since the 1987 National Academies report, Separation and Purification: Critical Needs and Opportunities. Many needs stated in the original report remain today, in addition to a variety of new challenges due to improved detection limits, advances in medicine, and a recent emphasis on sustainability and environmental stewardship. This report examines emerging chemical separation technologies, relevant developments in intersecting disciplines, and gaps in existing research, and provides recommendations for the application of improved separation science technologies and processes. This research serves as a foundation for transforming separation science, which could reduce global energy use, improve human and environmental health, and advance more efficient practices in various industries.